Instruction Manual

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1.16 Installing the filter cartridges .......................................................................................... 31
1.16.1 The role of the filter cartridge ..................................................................................... 31
1.16.2 Filter cartridge care instructions ................................................................................ 32
1.16.3 Filter cartridge installation .......................................................................................... 32
1.16.4 Adding a filter cartridge .............................................................................................. 33
1.16.5 Assigning filter descriptions in Quansoft .................................................................... 34
1.16.6 Editing filter descriptions ............................................................................................ 34
1.16.7 Removing a filter cartridge ......................................................................................... 34
1.16.8 Points to remember ................................................................................................... 35
1.17 Changing the instrument name ...................................................................................... 35
1.18 LED power settings ........................................................................................................ 37
1.19 Inserting a plate .............................................................................................................. 37
1.19.1 Inserting a previously run plate .................................................................................. 37
1.19.2 Running a plate .......................................................................................................... 37
1.20 After use ......................................................................................................................... 38
2 Getting started .......................................................................................................................... 39
2.1 Introduction to PrimeQ ....................................................................................................... 41
2.1.1 Principle of a real-time PCR instrument .................................................................... 41
2.1.2 Principle of PrimeQ .................................................................................................... 41
2.1.3 Applications of PrimeQ .............................................................................................. 41
2.2 Introduction to Real-time PCR ............................................................................................ 42
2.2.1 PCR ........................................................................................................................... 42
2.2.2 Qualitative vs. real-time PCR .................................................................................... 42
2.2.3 Real-time PCR on PrimeQ......................................................................................... 43
2.3 Overview of fluorescent chemistries................................................................................... 43
2.3.1 Intercalating dyes ....................................................................................................... 44
2.3.2 Fluorescent labelled probes ...................................................................................... 44
2.3.3 Molecular Beacons .................................................................................................... 45
2.4 System overview ................................................................................................................ 46
2.5 Optics module..................................................................................................................... 46
2.5.1 The optical light path .................................................................................................. 47
2.5.2 Filter cartridges .......................................................................................................... 47
2.5.3 Adjustable LED intensity ............................................................................................ 47
2.6 Thermal cycling module ..................................................................................................... 47
2.7 Scanning module ................................................................................................................ 48
3 Quansoft ................................................................................................................................... 49
3.1 Introducing Quansoft .......................................................................................................... 51
3.2 Software overview .............................................................................................................. 52
3.2.1 Home page ................................................................................................................ 53
3.2.2 The Editors ................................................................................................................ 53