Instruction Manual

39
2 Getting started
Introduction to PrimeQ and real-time PCR
About this chapter
This chapter gives a description of PrimeQ together with a basic
introduction to real-time PCR. It also provides examples of some of
the fluorescent chemistries that can be used with PrimeQ.
AGCTTAGGCCTAACCG
TCGAATCCGGATTGGCAGCTAAGGTTTCCAGATTCCAAATGCGCTA
AGCTTAGGCCTAACCGTCGATTCCAAAGGTCTAAGGTTTACGCGAT
GATTCCAAATGCGCTA
5’ 3’
5’
3’
Forward primer
Reverse primer
AGCTTAGGCCTAACCG
TCGAATCCGGATTGGCAGCTAAGGTTTCCAGATTCCAAATGCGCTA
AGCTTAGGCCTAACCGTCGATTCCAAAGGTCTAAGGTTTACGCGAT
GATTCCAAATGCGCTA
5’ 3’
5’
3’
Forward primer
Reverse primer
AGCTTAGGCCTAACCG
TCGAATCCGGATTGGCAGCTAAGGTTTCCAGATTCCAAATGCGCTA
AGCTTAGGCCTAACCGTCGATTCCAAAGGTCTAAGGTTTACGCGAT
GATTCCAAATGCGCTA
AGCTTAGGCCTAACCG
TCGAATCCGGATTGGCAGCTAAGGTTTCCAGATTCCAAATGCGCTA
AGCTTAGGCCTAACCGTCGATTCCAAAGGTCTAAGGTTTACGCGAT
GATTCCAAATGCGCTA
5’ 3’
5’
3’
Forward primer
Reverse primer
5’ 3’
5’
3’
Forward primer
Reverse primer