Instruction Manual

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3.16.5 Quick guide to plus-minus scoring analysis
1. In the Analysis Selection box, highlight the stage on which plus-minus scoring is to be
performed and click Edit .
2. In the Analysis Wizard Selection box, select Plus-minus scoring from the drop-down menu
and assign a use next to the appropriate dye(s) name. Click Next.
3. If a PRD was assigned, the PRD correction screen will be displayed. Choose whether to
perform a PRD correction on the data and click Next .
4. Baseline correction: Select which method to use (proportional is not an option in plus-minus
scoring) and click Next .
5. Choose a plus-minus analysis method and specify a range of readings if necessary. Click Next .
6. Set upper and lower confidence thresholds. Click Next .
7. Report options: Decide how to display the data in the PrimeQ report. Plus-minus score table is
the default setting but choose to display in graphical format. Click Next .
8. Summary of analysis. Click Finish to return to the Experiment or Results Editor main screen.