User Manual
36
OLIGO T
M
CALCULATOR
The oligo T
m
calculator can be used to estimate the T
m
of the PCR primers and help in selecting an
appropriate annealing temperature for the thermal cycling program.
•
Touch
Enter The Oligo Sequence
in order to
input the sequence of the primer.
•
Touch the
A C G T
buttons to enter the primer
sequence.
•
Touch
DEL
to delete the last character.
•
Touch
CLR
to delete the whole sequence.
•
Use the
arrow
keys to scroll through the
sequence.
•
Touch
OK
to return to the calculator screen.
•
Enter the
primer concentration
in the PCR mix.
•
Enter the
monovalent salt concentration
of
the PCR mix.
•
Touch
Calculate
to calculate the T
m
of the
primer.