Hybrigene Hybridisation oven OPERATOR’S MANUAL Issue 7 07/2014
CONTENTS INTRODUCTION ........................................................................................................ 5 BEFORE USE............................................................................................................. 6 Unpacking.................................................................................................................................................6 SAFETY AND INSTALLATION ................................................................................
DECLARATION OF CONFORMITY .........................................................................
INTRODUCTION Please read all the information in this manual before using the unit. The Techne Hybrigene hybridisation oven is ideal for blotting techniques in which RNA, DNA or protein are immobilised onto nylon or nitrocellulose filters. Several filters can be hybridised simultaneously in a single tube while the rotating design ensures volumes as low as 5ml can be used and recovered.
BEFORE USE Before using the Hybrigene please make sure you have read this manual carefully. If there is any doubt relating to the proper use of this equipment, the staff at Bibby Scientific Ltd. or your supplier will be happy to assist you.
SAFETY AND INSTALLATION Please read all the information in this manual before using the Hybrigene. WARNING HIGH TEMPERATURES ARE DANGEROUS: they can cause serious burns to operators and ignite combustible material.
I Mains Switch On O Mains Switch Off Replacement cable 2 Should the mains lead need replacement, a cable of 1mm of harmonized code H05VV-F connected to an IEC320 plug should be used. IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN.
SÉCURITÉ ET CONSIGNES D’INSTALLATION Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser le Hybrigene. AVERTISSEMENT Les TEMPÉRATURES ÉLEVÉES SONT DANGEREUSES car elles peuvent provoquer de graves brûlures chez l’opérateur et enflammer les matériaux combustibles.
3. Placer l’appareil sur une surface plane ou si nécessaire sous une hotte d’aspiration, veiller à ce que les trous d’aération situés sous l’appareil ne soient pas obstrués. 4. Raccorder le câble d’alimentation à la prise située à l’arrière de l’appareil. 5. Allumer l'appareil.
SICHERHEITS- UND INSTALLATIONSINFORMATIONEN Lesen Sie diese Anleitung vor Verwendung des Hybrigene bitte sorgfältig durch. ACHTUNG HOHE TEMPERATUREN STELLEN EINE GEFAHRENQUELLE DAR. Sie können schwere Brandverletzung verursachen und brennbare Stoffe entzünden. Techne hat bei der Konstruktion dieses Gerätes sehr darauf geachtet, daß der Bediener vor Gefahren geschützt ist.
I Netzschalter Ein O Netzschalter Aus Ersatzkabel 2 Bei einem eventuellen Austausch des Netzkabels wird ein Kabel vom Typ H05VV-F mit 1 mm Adernquerschnitt und Europastecker (IEC 320) benötigt. IM ZWEIFELSFALL EINEN ELEKTROFACHMANN HINZUZIEHEN.
INFORMAZIONI SULLA SICUREZZA E L'INSTALLAZIONE Leggere attentamente il presente manuale prima di usare il Hybrigene. AVVERTENZA Le ALTE TEMPERATURE SONO PERICOLOSE in quanto possono provocare serie ustioni agli operatori e dare fuoco al materiale combustibile.
3. Collocare l'unità su un banco piano idoneo o in una cappa aspirante se necessario, assicurandosi che gli sfiati delle prese d'aria nella parte inferiore non siano ostruiti. 4. Inserire il cavo di rete nella presa che si trova sul retro dello strumento. 5. Accendere lo strumento: I Interruttore di rete Acceso O Interruttore di rete Spento Cavo di ricambio 2 Qualora occorra sostituire il cavo di rete, si dovrà utilizzare un cavo di 1mm codice armonizzato H05VV-F collegato ad una spina IEC 320.
INFORMACIÓN DE SEGURIDAD E INSTALACIÓN Lea atentamente este manual antes de utilizar el Hybrigene. ADVERTENCIA LAS ALTAS TEMPERATURAS SON PELIGROSAS, ya que pueden ocasionar quemaduras graves a los operarios y prender el material combustible.
3. Sitúe la unidad sobre una mesa plana o en una campana de laboratorio si es necesario, y asegúrese de que los orificios de ventilación situados en la parte inferior no tienen ninguna obstrucción. 4. Conecte el cable de alimentación en el enchufe situado en la parte trasera del instrumento. 5.
CONTACT INFORMATION For technical, sales or servicing information, contact your local Techne dealer or: UK Bibby Scientific Ltd. Beacon Road Stone Staffordshire ST15 0SA UK Tel: +44 (0)1785 812121 Fax: +44 (0)1785 810405 E-mail: sales@bibby-scientific.com www.techne.com North and South America Bibby Scientific US Inc. t/a Techne Inc. 3 Terri Lane, Suite 10 Burlington, N.J. 08016 USA Toll Free (in NA): 800-225-9243 Tel: +1 609 589 2560 Fax: +1 609 589 2571 E-mail: labproducts@techneusa.com www.techneusa.
SPECIFICATIONS Tube capacity Maximum temperature* 4 (large and small in any combination) 16 mini tubes (when using the FHB61 mini tube holder) 64 x 15ml tubes (in holder) 32 x 50ml tubes (in holder) 80°C Minimum temperature 10°C above ambient Temperature selection Digital Adjustable rotation speed Adjustable rocking platform (optional) 0; 5 to 20 rpm 30 opm recommended Temperature display LED Temperature set point resolution 0.
INSTALLATION See also the sections on Safety and Installation. 1. Place the unit on a suitable flat bench or in a fume cupboard if required, ensuring that the air inlet and outlet vents are free from obstruction. 2. Place some water in a hybridisation tube and fit the tube in the Hybrigene. Adjust the feet on the base of the unit by unscrewing them anticlockwise slightly until the unit is level. Check the level by placing the tube in different positions in the oven. 3.
FHB4DSTK: Stacking and wall mounting kit for two units Description Wall Bracket M4 x 8 Pozi Pan Screw M4 Crinkle Washers Thumbscrew Wall Plate Stacking Plate Part code 6105214 6008652 6008639 6009552 6105213 6105212 Quantity 1 9 9 1 1 1 FHB4DKIT: Stacking and wall mounting kit for three units Description Wall Bracket M4 x 8 Pozi Pan Screw M4 Crinkle Washers Thumbscrew Wall Plate Stacking Plate Part code 6105214 6008652 6008639 6009552 6105213 6105212 Quantity 1 15 15 1 1 2 Fixing Procedure for two Hyb
8. Put the hybridisers in the position you want to use them. 9. Position the wall plate and mark the position of the holes. 10. Use the appropriate screws and fixings to attach the wall plate to the wall; ensure that the screws are tight. Wall plate Wall bracket Wall fixings Hybrigene with wall bracket attached 11. Re-position the hybridisers placing the wall bracket over the wall plate. 12. Use the thumb-screw to attach the wall bracket to the wall plate.
SETTING THE TEMPERATURE Controller display 1. Press the ’P’ button on the controller and the set temperature will be displayed. 2. To increase the set temperature, press the Increase button. 3. To decrease the set temperature, press the Decrease button. 4. Press P and the unit will now go to the set temperature. 5. If no key is pressed for 5 seconds the display will return to show the actual temperature and the unit will go to the new set temperature. 6.
HYBRIDISATION TUBES The hybridisation tubes are manufactured from durable thick borosilicate glass with a non-drip design. The tubes must be checked regularly and replaced if any damage occurs. Any damage to the ends of the tubes can cause them to break or leak when the end caps are inserted. Large (80mm diameter) tube assembly (FHB16) Remove the tube end cap which has the two holes in it. Place the filters in the tube and replace the end cap.
Mini (4 x 32mm) tube assembly (FHB41 and FHB61) The mini tubes must be used in the FHB61 multi-tube holder. Unscrew the end cap of the tube. Place the filters and the liquids appropriate to the protocol in the tube and replace the end cap. Fix the tube into a multi-tube holder, using the springs, and ensure that there are four tubes in the holder. The end caps must be as shown, on alternate ends. The multi-tube holder will not rotate properly if there are not four mini tubes in it.
INSTALLING THE HYBRIDISATION TUBES 1. Pre-heat the oven to the required temperature. 2. Insert the tubes or tube holder into the Hybrigene ensuring that they rotate freely and the end caps engage on the drive rollers on the right had side of the chamber. 3. Close the door. 4. Turn the speed control knob until you reach the required speed.
DO NOT TIP THE TUBE SO THAT THE COLOURED END IS DOWN AS THIS WILL CAUSE A SPILLAGE. 3. The unit has been designed to conform to international safety specifications and is fitted with an over-temperature cut-out. If, for any reason, the temperature rises above a safe level for the instrument, the over temperature protection cuts out the heater, but the tubes continue to rotate. Check the reason for the cut out and, if there is no obvious cause, turn the unit off, allow it to cool then turn it on again.
ADDITIONAL INFORMATION Note that this equipment should only be dismantled by properly trained personnel. Removing the outer cover exposes potentially lethal mains voltages. There are no user serviceable parts within this equipment. FAULT FINDING Should you have any problems with your Hybrigene which cannot be easily remedied, you should contact your supplier and return the unit if necessary. Contact the Bibby Scientific Ltd.
SPARES AND ACCESSORIES The following spares and accessories may be purchased from Bibby Scientific Ltd. or their dealers. All Techne tubes are made from durable Borosilicate glass. Contact Bibby Scientific Ltd. or your Techne dealer for further details. Part code Description FHB16 Large (80mm diameter) hybridisation tube assembly complete with sealing rings and end caps.
DECLARATION OF CONFORMITY 29
