User Manual

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Survivor Filter Report No.:102092758COL-002
Date: June 12, 2015 Page 3 of 8
GFT-OP-10b (12 April 2013).
Benchmark and Non-Standard test Report: Report must be reproduced in its entirety
Objective
The primary objective of this evaluation is to provide test data showing the amount of bacteria, virus, and heavy
metals (Lead, Mercury and Cadmium) removed from a water matrix by the Survivor Filter and Survivor Filter Pro.
Overview
A challenge suspension of known concentration will be prepared and introduced into the system. The suspension
will be run through the filter and then analyzed for remaining concentrations.
Separate and new filters are to be used for the bacteria, virus, and heavy metals.
Detection will be as follows:
I. Bacteria
a. Collection and enumeration technique derived from the Standard Total Coliform Membrane
Filter Procedure in accordance with APHA’s Standard Methods for the Examination of Water and
Wastewater. This procedure is commonly used for NSF/ANSI certification standards for food
equipment.
II. Virus
a. Collection and enumeration technique derived from the Standard Total Coliform Membrane
Filter Procedure in accordance with APHA’s Standard Methods for the Examination of Water and
Wastewater. This procedure is commonly used for NSF/ANSI certification standards for food
equipment.
III. Heavy Metals
a. The Elemental analysis of the Lead, Mercury and Cadmium will follow the IEC 62321 Elemental
analysis procedure. This procedure is commonly used for RoHS analysis of various materials.
Procedural
I. Bacterial Analysis
a. Challenge Suspension Preparation
1. E. coli, (ATCC 11229) and S. aureus (ATCC 6538) bacteria obtained from ATCC
2. Stock cultures rehydrated with Tryptic Soy Broth (TSB) and incubated at 36 ± 1 °C (97 ± 1
°F) for approximately 24 hours
3. The suspension will be diluted with phosphate buffer solution (PBS) to yield a
concentration of 1 to 5x10
6
cfu/mL of each organism
b. Test
1. 100 mL of the challenge suspension is filtered through the Survivor unit
2. Samples will be serial diluted and will be plated on both MacConkey’s agar and Mannitol
Salts agar, inverted, and incubated at 36 ± 1 °C (97 ± 1 °F) for 24 h
i. Serial dilution plating will be performed in duplicate
3. Plates are to be enumerated after the incubation period and results expressed as the
number of CFU/mL
i. Only plates within the range of 20-300 cfu will be reported
4. Control Samples to be performed as follows:
i. Negative control: PBS will be filtered through unit and enumerated
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