User's Manual

3-68 KleeYa - User Manual - Version 2
BASIC FUNCTIONS
RESULTS
3.5.3 CALIBRATION AND MASTER CURVES
The Calibration Curves screen and the Master Curves screen show information of
all created calibration curves and the underlying master calibration curves.
Figure 3-61: Results - Calibration Curves screen
Calibration curves (also standard curves or working curve) are used to compensate
the differences between reagent lots, different analyzers and environmental
conditions. To generate a calibration curve an assay calibration must be run and
validated according to the indications reported in the instructions for use of the
assay (indications may vary per assays).
The results are calculated in comparison to a calibration curve.
The specific reference curve (master curve) for a reagent lot is provided by the
vendor of the Reagent Cartridge (see instruction for use of the relevant assay for
details). If a new reagent lot is used, this curve must be loaded on the database and
then adjusted by a calibration before sample results can be calculated.
The measuring signals of the calibrators allow the shift of all master curve points to
a working curve, corresponding with the actual conditions during measurement.
The stored master curve is generally specified with a defined number of master
curve base points.
• In dependence of the assay a pre-defined number of calibrators with known
concentration values are measured. These measured signals (RLU) are
compared with the master curve signal of the corresponding calibrator
concentrations.
• The relative difference between the measured RLU and the master RLU of the
calibrators is calculated.
• Based on appropriate compensation factors, a re-adjustment of the master
curve points is made in order to achieve, the working curve.