Click for English Guides Summary USER’S GUIDE AUTOTUNE SERIES HIGH INTENSITY ULTRASONIC PROCESSOR WITH TEMPERATURE CONTROLLER 250 Watt Model • 500 Watt Model 600 Watt Model • 750 Watt Model TABLE OF CONTENTS Warranty Important Safeguards Low Surface Tension Liquids - Organic Solvents SECTION 1 – INSTALLATION Inspection Electrical Requirements Installing the Ultrasonic Processor SECTION II – OPERATION Principles of Ultrasonic Disruption Functions of Controls, Indications, and Connectors Preparations for Use
WARRANTY Your Ultrasonic Processor is warranted and backed by the manufacturer for a period of three years from the date of shipment against defects in material and workmanship under normal use as described in this instruction manual.
IMPORTANT SAFEGUARDS READ BEFORE INSTALLING OR USING THE EQUIPMENT Your Ultrasonic Processor has been designed with safety in mind. However, no design can completely protect against improper usage, which may result in bodily injury and/or property damage. For your protection and equipment safeguard, observe the following warnings at all times, read the operating instructions carefully before operating the equipment, and retain this instruction manual for future reference.
CAUTION LOW SURFACE TENSION LIQUIDS – ORGANIC SOLVENTS The probes (solid or with a replaceable tip) are tuned elements that resonate at a specific frequency. If the replaceable tip is removed or isolated from the rest of the probe, the element will no longer resonate at that frequency, and the power supply will fail. Unlike aqueous (water based) solutions which rarely cause problems, solvents and low surface tension liquids are problematic.
ELECTRICAL REQUIREMENTS The Ultrasonic Processor requires a fused, single phrase 3-terminal grounding type electrical outlet capable of supplying 50/60 Hz at 100 volts, 115 volts, 220 volts, or 240 volts, depending on the voltage option selected. For power requirements, check the label on the back of the unit. WARNING For your personal safety, do not, under any circumstances, defeat the grounding feature of the power cord by removing the grounding prong.
SECTION II – OPERATION PRINCIPLES OF ULTRASONIC DISRUPSION The ultrasonic power supply converts 50/60 Hz line voltage to high frequency electrical energy. This high frequency electrical energy is transmitted to the piezoelectric transducer within the converter, where it is changed to mechanical vibrations. The vibrations from the converter are intensified by the probe, creating pressure waves in the liquid.
FUNCTIONS OF KEYS, CONTROLS, INDICATIONS, AND CONNECTORS FRONT PANEL LCD screen 0–9 key CLEAR key ENTER REVIEW key Displays prompts and the following control parameters: • Amplitude selected • Output power delivered to the probe in watts, and as percentage of the total power • Selected duration of processing • Actual processing time • Elapsed time • Set and read temperature • Pulse duration • Accumulated amount of energy in Joules delivered to the probe. Input digits. Clears preceding entry.
FUNCTIONS OF KEYS, CONTROLS, INDICATIONS, AND CONNECTORS (cont.) REAR PANEL Footswitch jack Connects to external actuation device, and enable power and frequency monitoring. Connects to the footswitch cable. Coax connector Connects to the converter. Power module Connects to the electrical line cord and encases the fuse(s). 9 pin D-sub connector 9-PIN D-SUB CONNECTOR Pin No. 1 2 3 4 5 6 7 Description Not connected Not connected Not connected Enables connection to a frequency counter.
PREPARATION FOR USE CAUTION Do not operate an Ultrasonic Processor that has been in a very cold or hot environment for a prolonged period of time. Wait until it has reached room temperature 1. Ensure that the AMPLITUDE dial is set fully counter-clockwise. 2. Plug the electrical line cord into the electrical outlet. 3. If the optional footswitch is used, insert the plug into the jack located on the rear panel. Make sure that the plug is inserted forcefully all the way in. 4.
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No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 DESCRIPTION Converter Model CV33 Four element coupler Stepped top(s) 1/8” (3mm) Booster Probe ½” (13mm) solid Probe ½” (13mm) with threaded end and replaceable tip Probe ¾” (19mm) solid Probe ¾” (19mm) with threaded end and replaceable tip Probe 1” (25mm) solid Probe 1” (25mm) with threaded end and replaceable tip Replaceable tip ½” (13mm) Replaceable tip ¾” (19mm) Replaceable tip 1” (25mm) Coupler Stepped tip 1/8” (3mm) Probe ½” (13mm) with threaded
USING THE ULTRASONIC PROCESSOR The speed control on an automobile, can, to a certain extent, be compared to an Ultrasonic Processor. The speed control is designed to maintain the vehicles rate of travel constant. As the terrain changes, so do the power requirements. The speed control senses these requirements, and automatically adjusts the amount of power delivered by the engine in order to compensate for these ever changing conditions.
• • • • • CAUTION Never allow liquid to spill into the converter. Do not use the cup horn without a splash shield Do not allow a microtip or extender to vibrate in air for more than 10 seconds. When working with a microtip never allow the AMPLITUDE control to be set above the microtip limit 40%. Ignoring these instructions will cause the microtip to fracture. Do not allow the vibrating microtip to contact anything but the sample.
AMPLITUDE: The amplitude is the only parameter that must be set in order for the Ultrasonic Processor to be operational. The other control parameters – Time and Pulse, do not have to be set for continuous operation. AMPL. displays the percentage of maximum of amplitude e.g. 40%, set by the AMPLITUDE control. Rotate the AMPLITUDE control for a 40% reading on the LCD screen – Ampl 40%.
NOTE The probe is tuned to vibrate at a specific frequency. If the resonant frequency of the probe has changed, due to cavitation erosion or fracturing, a minimum reading will not be obtained. If an overload condition exits, or if minimum reading cannot be obtained (less than 20%) with the probe out of the sample, check the instrument without the prove to determine which component might be defective.
PULSER: By inhibiting heat build-up in the sample, the pulse function enables safe treatment of temperature sensitive samples at high intensity. In addition, pulsing enhances processing by allowing the material to settle back under the probe after each burst. The ON and OFF pulse duration can be set independently from .1 second to 9.9 seconds. During the OFF portion of the cycle, the red indicator on the PULSER key will illuminate.
TEMPERATURE: The temperature function prevents overheating of the sample by continuously monitoring the sample temperature, and terminating the ultrasonics when the temperature reaches a predetermined setpoint. The ultrasonics is automatically reinstated when the temperature drops below the setpoint.
NOTE The amount of energy displayed will be only for one cycle. Initiating a new cycle will reset the display to zero. SAVE: The save function retains in memory up to 10 control parameters under a storage identification (ID) number. To store the parameters under an ID number, (0-9) press the SAVE key. The indicator light on the SAVE key will illuminate and the screen will display. e.g. TIME 5:30:25 #__ PULSE On 2.5 TEMP 35° C AMPL 40 % Using the numeric keys, enter the ID number. e.g.
RECALL: The recall function can retrieve from memory; any of the 10 stored control parameters for verification or usage. To retrieve any parameters press the RECALL key. The indicator light on the RECALL key will illuminate, and the screen will display: e.g. ID TIME __:__:__ # __ PULSE On __:__ TEMP _ _° C Off __:__ Using the ID number and the numeric keys, select the desired parameters, which must be retrieved. The screen will display: e.g. ID #7 TIME 5:30:25 PULSE On 2.5 TEMP 35° C Off 1.
IMPORTANT Proper care of the probe is essential for dependable operation. The intense cavitation will, after a prolonged period of time, cause the tip to erode, and the power output to decrease without showing up on the wattmeter. The smoother and shinier the tip, the more power will be transmitted into the sample. Any erosion of the probe tip will increase the rate of future erosion.
SECTION III – SERVICE INFORMATION Your Ultrasonic Processor was designed to provide you with years of safe and dependable service. Nevertheless, because of component failure or improper usage, the possibility does exist that it might not perform as it should, shut down due to an overload condition or that it will stop working all together. The most probable causes for malfunction are listed below and should be investigated.
RETURN OF EQUIPMENT It is suggested that an Ultrasonic Processor in need of repair be sent back to the factory. In order to receive prompt service; always contact the factory before returning any instrument. Include date of purchase, model number and serial number. For instruments not covered by the warranty, a purchase order should be forwarded to avoid unnecessary delay. Care should be exercised to provide adequate packing to insure against possible damage in shipment.
TEMPERATURE PROBE CALIBRATION PLACE TEMPERATURE PROBE INTO ICE WATER BATH 5. Immerse the temperature Probe in the center of the ice water bath for a period of 40 seconds. Do not allow the probe to contact the vessel. When the selfcalibration for low temperature is complete, the screen will display the following message: PLACE TEMPERATURE PROBE INTO BOILING WATER 6. Immerse the Temperature Probe in the center of the boiling water for a period of 40 second. Do not allow the probe to contact the vessel.
SECTION IV - OPERATING SUGGESTIONS AND TECHNIQUES DISRUPTING CELLS Single-cell organisms (micro-organisms) consist of a semipermeable, tough, rigid outer cell wall surrounding the protoplasmic membrane and cytoplasm. The cytoplasm is made up of nucleic acid, protein, carbohydrates, lipids, enzymes, inorganic ions, vitamins, pigments, inclusion bodies, and about 80% water.
2369 USA, phone (800) 221-2574 or (601) 939-4612, FAX (601) 932-5339, Jayco Inc. 675 Rahway Ave., Union NJ 07083 USA, phone (908) 688-3600, FAX (908) 688-6060 or Sigmund Lindner GmbH. P.O. Box 29. D-95483 Warmensteinach, Germany. Phone (49) 0 92 77 9 94 10, FAX (49) 0 92 77 9 94 99. When processing difficult cells, pretreatment with an enzyme such as lysozyme or byaluronidase might be beneficial.
Since the greatest concentration of energy is beneath the probe, it is imperative that the sample be kept as close to the tip as possible, liquids are easily processed because the free moving cells circulate repeatedly below the probe. Solid materials however have a tendency to be repelled by the ultrasonic, and should be processed in a vessel large enough to accommodate the probe, yet small enough to restrict sample movement. For small samples, conical shaped test tubes are recommended.
to optimize cooling. Processing samples in a Cup Horn will usually take 3 to 4 times longer than processing with direct probe intrusion.
