Third Party Verification Claim

NON-GLP STUDY REPORT Date: June 2016
Sponsor: Sprinkler Magician
Study: Direct Spray 16
Trial: AEDSAE
Sponsor Code: N/A
Test Method: 311
MATERIALS AND METHODS:
The following is the Snell Scientifics Standardized Testing Method for evaluating the efficacy of
pesticides when applied as direct spray applications against various arthropod species. Further details
related to this specific study are described following the test method summary. Select action items and
illustrations have been removed from this standardized test method in an effort to make the report
more precise and accurate to the study conducted. Any details removed from this test method were
deemed irrelevant to the study conducted in this report.
311.1 Materials:
Test Arena Information:
311.1.1 Treatment Arenas: 1.75” CPVC Mesh Cartridge (BioQuip 7250NSW mesh). The Test Arenas
were used to contain the test systems during the test substance applications.
311.1.2 Post-Treatment Arenas: 20oz SOLO cup with lid. The Post-Treatment arenas were used to
contain the test systems in a clean environment after exposure to the test substance(s).
311.1.3 Food/Moisture: 10% sucrose soaked cotton swabs.
Test Equipment:
311.1.4 Volumetric Measuring Equipment: Graduated cylinders and/or beakers were used as
needed in preparing and/or measuring the flow rates of the test substance(s).
311.1.5 Digital Balance(s): Balances were used as needed in preparing and/or weighing the test
substance canisters before and after applications.
311.1.6 CO
2
and Regulator: A standard 20 pound CO
2
cylinder with regulator was used to
anesthetize the test systems and sort them into the test arenas (prior to exposure to the
test substances). The test systems were allowed to adequately recover from anesthetizing
before being exposed to the test substance(s), and they were not anesthetized at any point
following exposure to the test substance(s). Any additional transfers required after
exposure to the test substances was conducted using methods that did not involve
anesthetizing.
311.1.7 Intermediate Sorting/Transfer Containers: Additional sorting and transfer containers were
used to aid in moving the test systems from the primary rearing/collection containers and
into the treatment and/or post-treatment arenas.
311.1.8 Metronome/Timing Equipment: A metronome and/or other timing equipment were used as
needed to assist in the timing when conducting the applications and/or when collecting the
observations.
Application Equipment:
311.1.9 Application Equipment: Snell Sci. Trigger Sprayer.