Instruction Manual

11
6. Create fi ve 10-fold serial dilutions (to 10
-5
of original suspension) from each
suspension and spread 0.1 ml of each dilution (plus 0.2 ml of undiluted sample
if a low bioaerosol count is expected) onto the surface of duplicate agar plates.
Select agar media based on the expected microbial species.
7. Incubate plates at appropriate temperatures and count emerging colonies a er
four to seven days of incubation. Use total number of CFU per plate to calculate
airborne viable cell concentrations. Samples can be stained and analyzed with
epifl uorescent microscope.
References
1
Kenny, L.C., Bowry, A., Crook, B., and Stancliff e, J.D., “Field Testing of a Personal Size-
selective Bioaerosol Sampler,” American Occupational Hygiene, Vol. 43, No. 6, 1999, pp.
393-404
2
Kenny, L.C., Stancliff e, J.D., Crook, B., Staff , S., Griffi ths, W.D., Stewart, I.W., and Fut-
ter, S.J., “The Adaptation of Existing Personal Inhalable Aerosol Samplers for Bioaerosol
Sampling,” American Industrial Hygiene Association Journal, Vol. 59, 1998, pp. 831-841
3
Kenny, L.C., Chung, K.Y.K., and Dilworth, M., “Applications of Low-cost Multifraction
Aerosol Samplers - Final Report,” IR/EXM/99/06, Health and Safety Laboratory, U.K.,
1999
4
Kenny, L.C., Chung, K.Y.K., Dilworth, M., Hammond, C., Jones, J. Wynn, Shreeve, Z.,
and Winton, J., “Applications of Low-cost Dual-fraction Dust Samplers,” Ann. Occup.
Hyg., Vol. 45, No. 1, 2001, pp. 35-42
5
Mark, D. and Vincent, J. H., “A New Personal Sampler for Airborne Total Dust in Work-
places,” Ann. Occup. Hyg. Vol. 30, 1986, pp. 89-102
6
ACGIH Technical Commi ee on Air Sampling Procedures: Particle Size-selective Sampling
in the Workplace, ACGIH, Cincinnati, Ohio, 1984