Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Laboratory Ventilation Codes and Standards
Siemens Industry, Inc. 80
Topic Requirement(s) Commentary
Biological
Safety
Cabinets and
Classifications
(Continued)
National Sanitation Foundation, NSF 49 -2008, Biosafety Cabinetry: Design,
Construction, Performance and Field Certification
3 Definitions
3.4.1 Class I: A ventilated cabinet for personnel and environmental protection,
having an unrecirculated inward airflow away from the operator that exhausts all
air to the atmosphere after filtration through a HEPA filter. Class I cabinets are
suitable for work where no product protection is required.
NOTE – Although the traditional Class I BSC is exhausted to the atmosphere
without recirculation into the lab, it is recognized that some of the benefits of the
Class I BSC can be obtained even when the unit’s HEPA filtered exhaust is
vented back into the laboratory.
3.4.2 Class II:
A ventilated cabinet for personnel, product, and environmental protection having
an open front with inward airflow for personnel protection, downward HEPA
filtered laminar airflow for product protection, and HEPA filtered exhausted air for
environmental protection.
NOTE – When toxic chemicals or radionuclides are used as adjuncts to biological
studies or pharmaceutical work, Class II cabinets designed and constructed for
this purpose should be used.
3.4.2.1 Class II Type A1 cabinets (formerly designated Type A): cabinets that:
– maintain minimum average inflow velocity of 75 ft/min (0.38 m/s) through the
work access opening;
– have HEPA filtered downflow air that is a portion of the mixed downflow and
inflow air from a common plenum (i. e., a plenum from which a portion of the air
is exhausted from the cabinet and
the remainder supplied to the work area);
– may exhaust HEPA filtered air back into the laboratory or to the environment
through an exhaust canopy; and
– have all biologically contaminated ducts and plenums under negative pressure
or surrounded by negative pressure ducts and plenums.
Type A1 cabinets are not suitable for work with volatile toxic chemicals and
volatile radionuclides.
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