Basic Documentation
Table Of Contents
- Introduction
- Applicable Definitions (Alphabetical Listing)
- Laboratory Safety
- Hazard Assessment
- Chemical Hygiene Plan
- Chemical Hygiene Responsibilities
- Fume Hoods
- When Required & Safe Usage
- Gloveboxes:
- Face Velocity
- Face Velocity Setback
- Size & ADA Compliance
- CAV (Constant Air Volume) Bypass
- CAV (Constant Air Volume) Conventional
- VAV (Variable Air Volume)
- VAV Diversity
- Automatic Sash Closure
- Safe Operation of Sashes
- Accessories, Services and Explosion Protection
- Ductless
- Auxiliary Air
- (Special Purpose) Perchloric Acid
- Room Air Cross Currents
- Minimum Exhaust
- Monitoring
- Selection Criteria and Performance Specifications
- Laboratory Design & Fume Hood Implementation
- Maintenance
- Periodic Testing
- Test Procedures
- Signage and Recordkeeping
- Shutdown Procedures
- Evaluating CAV (Constant Air Volume) Systems
- Evaluating VAV (Variable Air Volume) Systems
- Biological Laboratories
- Biosafety Level 1
- Biosafety Level 2
- Biosafety Level 3
- Biosafety Level 4
- Ventilation for Biosafety Level 1
- Ventilation for Biosafety Level 2
- Ventilation for Biosafety Level 3
- Ventilation for Biosafety Level 4, Cabinet Laboratory
- Ventilation for Biosafety Level 4, Suit Laboratory
- Containment Levels - Canada
- Containment Levels and Ventilation Requirements: Canada
- Biological Safety Cabinets and Classifications
- Biosafety Cabinet Applications
- Biosafety Cabinets – Installation and Safe Usage Recommendations
- Biosafety Cabinets – Certification and Safe Usage - Canada
- Biological Safety Cabinet Design, Construction and Performance Requirements
- Biosafety Cabinet Testing
- Ventilation Systems
- Local Ventilation -When Required
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Animal Rooms
- Ventilation Rates for Biological Labs
- Ventilation Rates for Chemical Laboratories
- Ventilation rates for Storage areas
- Room Supply Air
- Supply Air Quality and Filtration
- Room and Duct Pressurization
- Human Occupancy, Room Temperature and Humidity
- Animal Rooms Room Temperature and Humidity
- Load Calculations
- Room Sound Level and Vibration
- Emergency Control Provisions
- Energy Conservation
- Monitoring
- Maintenance
- Periodic Inspection and Testing
- Periodic Inspection and Testing - Canada
- Test Records
- Management
- Exhaust Systems
- Configuration
- Leakage
- Components
- Manifolded Systems
- Air Velocity
- Stack Height and Discharge Location
- Operational Reliability
- Recirculated Air and Cross Contamination
- Materials and Fire Protection
- Commissioning
- Commissioning - Canada
- Referenced Publications
Biological Laboratories
Siemens Industry, Inc. 79
Topic Requirement(s) Commentary
Biological
Safety
Cabinets and
Classifications
(Continued)
The Class II, Type A2 BS.C (Formerly called A/B3): Only when this BSC is ducted
to the outdoors does it meet the requirements of the former Class II Type B3.8 The
Type A2 cabinet has a minimum calculated or measured inflow velocity of 100 fpm.
All positive pressure contaminated plenums within the cabinet are surrounded by a
negative air pressure plenum thus ensuring that any leakage from a contaminated
plenum will be drawn into the cabinet and not released to the environment. Minute
quantities of volatile toxic chemicals or radionuclide can be used in a Type A2
cabinet only if it exhausts to the outside via a properly functioning canopy
connection.
Class III
The Class III BSC was designed for work with highly infectious microbiological
agents and for the conduct of hazardous operations and provides maximum
protection for the environment and the worker. It is a gas-tight (no leak greater than
1x10-7 cc/sec with 1% test gas at 3 inches water gauge enclosure with a non-
opening view window. Access for passage of materials into the cabinet is through a
dunk tank, that is accessible through the cabinet floor, or double-door pass-through
box (e.g., an autoclave) that can be decontaminated between uses. Reversing that
process allows materials to be removed from the Class III BSC safely. Both supply
and exhaust air are HEPA filtered on a Class III cabinet. Exhaust air must pass
through two HEPA filters, or a HEPA filter and an air incinerator, before discharge
directly to the outdoors. Class III cabinets are not exhausted through the general
laboratory exhaust system. Airflow is maintained by an exhaust system exterior to
the cabinet, which keeps the cabinet under negative pressure (minimum of 0.5
inches of water gauge.) Long, heavy-duty rubber gloves are attached in a gas-tight
manner to ports in the cabinet to allow direct manipulation of the materials isolated
inside. Although these gloves restrict movement, they prevent the user’s direct
contact with the hazardous materials. The trade-off is clearly on the side of
maximizing personal safety. Depending on the design of the cabinet, the supply
HEPA filter provides particulate-free, albeit somewhat turbulent, airflow within the
work environment. Laminar airflow is not a characteristic of a Class III cabinet.
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