HIGH-HEAT DECONTAMINATION CO2 INCUBATOR 110 - 120 Voltage Installation and Operation Manual SCO6AD Previously designated: 3552
SCO6AD HIGH-HEAT DECONAMINATION CO2 INCUBATOR Installation and Operation Manual Part Number (Manual): 4861712 Revision: December 22, 2014 These units are TÜV CUE listed as air jacketed CO2 Incubators for professional, industrial, or educational use where the preparation or testing of materials is done at approximately atmospheric pressure and no flammable, volatile, or combustible materials are being heated. These units have been tested to the following requirements: CAN/CSA C22.2 No.
TABLE OF CONTENTS INTRODUCTION........................................................................................................................................... 5 General Safety Considerations ................................................................................................................. 5 Engineering Improvements ....................................................................................................................... 6 Contacting Assistance .........................
Temperature ............................................................................................................................................ 43 Power ...................................................................................................................................................... 43 Conversion Table .................................................................................................................................... 43 PARTS LIST ................................
INTRODUCTION Thank you for purchasing a Sheldon SCO6AD High-Heat Decontamination CO2 incubator. We know that in today’s competitive market place, customers have many choices when it comes to constant temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests with you. Sheldon Manufacturing, Inc. stands behind our products and want to let you know we are here if you need us.
INTRODUCTION (CONTINUED) ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products. As a result, engineering changes and improvements are made from time to time. Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit’s operating characteristics or appearance differs from those described in this manual, please contact your Shel Lab dealer or distributor for assistance.
RECEIVING YOUR INCUBATOR Before leaving the factory, SCO6ADs are packaged in high-quality shipping materials to provide protection from transportation-related damage. When an incubator leaves our factory, safe delivery becomes the responsibility of the carrier. Damage sustained during transit is not covered by the manufacturing defect warranty. When you receive your SCO6ADs inspect it for concealed loss or damage to the interior and exterior.
RECEIVING (CONTINUED) INSPECTION AND ORIENTATION Side Access Port with Copper Filter OTL and Data Sample Panel Main Control Panel CO2 Panel Temperature Decontamination Cycle Panel Panel Incubation Chamber Interior Glass Viewing Door Exterior Door Gasket Shelf Standard Exterior Door Latch Exterior Door Inner Door Latch Inner Door Gasket Humidity Pan with Copper Token 8|Page
RECEIVING (CONTINUED) RECORDING DATA PLATE INFORMATION Locate the data plate on the right side of the unit, above the copper-filtered access port. The data plate contains the incubator model number and serial number. Enter this information below for future reference.
GRAPHIC SYMBOLS (CONTINUED) The incubator is provided with multiple graphic symbols on its exterior and internal surfaces. These symbols identify hazards, and the functions of the adjustable components, as well as important notes in the user manual. Symbol Definition Indicates that you should consult your service manual for further instructions. Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions complémentaires.
GRAPHIC SYMBOLS (CONTINUED) Symbol Definition Indicates Potential Shock Hazard Signale danger électrique WEEE Directive compliant logo Indicates the unit should be recycled (Not disposed of in land-fill) Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge) Indicates CO2 Gas Indique gaz CO2 Indicates carbon dioxide gas content as a % Indique le gaz carbonique contenu en % Indicates USB Port Indique Port USB 11 | P a g e
INSTALLATION AMBIENT CONDITIONS The SCO6AD is intended for use indoors at room temperatures between 15C and 30C (59F and 86F), at no greater than an ambient 80% Relative Humidity (at 25C / 77F). Allow a minimum of 4 inches (10cm) between the incubator and walls or partitions, and 2 inches (5cm) of clearance above the top of the incubator for unobstructed airflow.
INSTALLATION (CONTINUED) LIFTING The incubator should only be lifted by its bottom surfaces using proper heavy lifting machinery such as, a forklift or pallet jack. Handles and knobs are inadequate for lifting or stabilization. The unit should be completely restrained from tipping during lifting. Transporting the unit while lifted is not recommended and may be hazardous. Remove all moving parts, such as shelves and trays, and secure the door in the closed position prior to lifting or transporting the unit.
INSTALLATION (CONTINUED) CONNECT THE CO2 SUPPLY TO THE INCUBATOR Note: Always use medical grade CO2. Use of non-medical grade CO2 risks introducing contaminants into the chamber, may damage the incubator, and will void the incubator’s warranty. Failure to use the in-line HEPA filter also voids the warranty. Always install the in-line HEPA filter (Part Number 2800525) provided with the SCO6AD tubing kit between the gas source and the incubator. The filter is directional.
INSTALLATION (CONTINUED) INSTALLATION CLEANING AND DISINFECTION If required by your laboratory protocol, clean and manually disinfect the incubator chamber and shelving components. Cleaning and disinfecting during installation reduces the risk of contamination. The chamber and shelving were cleaned at the factory, and a decontamination cycle was run to verify the cycle functionality. However, Sheldon Manufacturing cannot guarantee that the incubator was not exposed to contaminants during shipping.
CONTROL PANEL OVERVIEW OTL - Data Panel (Left) and Control Panels OTL - Data Panel Over Temperature Limit Thermostat (OTL) Labeled Set Over Temp Control, the OTL section of the panel is provided with a graduated dial. The OTL system is an independent backup heating control. When set, it guards against the chamber temperature rising more than 1°C above the main temperature control set point. Please see the Over Temperature Limit System description in the Theory of Operations (page 21).
CONTROL PANEL OVERVIEW (CONTINUED) Temperature Control and Display Panel Labeled Set Temperature °C, this panel comes with a green digital display that shows the air temperature in the incubation chamber, accurate to 0.1°C. The display can also show the userselected temperature set point, as well as display-adjustments during calibrations. The controls consists of an UP / DOWN arrow pad for inputting a temperature set point, muting active temperature deviation alarms, and performing calibrations.
CONTROL PANEL OVERVIEW (CONTINUED) Fuse Located inside the black cord inlet on the back of the incubator, the fuse protects against overcurrent conditions. If the fuse blows, the incubator will shut down. For your safety, the cause of a blown fuse should be determined prior to replacing it. Always de-power and unplug the incubator prior to accessing the fuse holder.
OPERATION THEORY OF OPERATION The SCO6AD is engineered to provide a constant temperature CO2 incubation environment. The unit can obtain a stable, uniform (±0.25°C) temperature in its chamber, ranging from the room temperature +5°C up to 50°C for incubation applications, when properly calibrated. The unit is also provided with an automated high-heat decontamination cycle that does not require the removal of the CO2 sensor or other electronic components.
OPERATION (CONTINUED) CO2 Atmosphere The SCO6AD controls the concentration of CO2 in the incubation chamber atmosphere by operating an internal electric gas pump connected to the gas input line. The motor pulses CO2 into the chamber air stream when deviations from the user-programed CO2 set point are detected. The incubator monitors the chamber CO2 concentration with an infrared sensor in a recirculation box above the main chamber.
OPERATION (CONTINUED) Humidification Humidification is provided by filling the humidifying pan included with the unit. The pan is then placed on the heated chamber floor. Evaporation driven in part by heating raises the relative humidity percentage (RH%) of the chamber. A copper anti-microbial token helps to retard the growth of microbiological organisms in the pan. Decontamination Cycle The SCO6AD comes with a user-initiated eight-hour high heat cycle designed to kill microbiological organisms.
OPERATION (CONTINUED) PREPARING THE INCUBATOR FOR USE Note: The preparation procedure requires approximately 24 hours to complete. This includes at least 8 hours for the incubator temperature, humidity, and CO2 levels to stabilize prior to temperature and gas calibrations, and 8 hours to run a decontamination auto cycle.
OPERATION (CONTINUED) Preparation Procedure Continued Complete the following procedures in the Operation section: a. Set the Temperature Set Point page 24 b. Calibrate the Temperature Display page 25 c. Set the Over Temperature Limit page 27 d. Set the CO2 Set Point page 29 e. Calibrate the CO2 Display page 30 f.
OPERATION (CONTINUED) SET THE TEMPERATURE SET POINT The unit comes from the factory with a set point of 37°C. Perform the steps below to change the set point to the operational temperature you will be using for your incubation application. This also prepares the unit for temperature calibration. The example below assumes the incubator will be run at 39°C during normal use. Turn the Over Temperature Limit control dial clockwise to the maximum position indicated by the largest dot. a.
OPERATION (CONTINUED) CALIBRATE THE TEMPERATURE DISPLAY The SCO6AD was calibrated at the factory to 37°C. The incubator should be calibrated as required by regulatory compliance and your laboratory protocol, or whenever the unit is installed in a new working environment. Calibrate the incubator at the temperature set point of your incubation. Use a certified temperature-sensing reference device regularly calibrated to 0.1°C by an independent party.
OPERATION (CONTINUED) Temperature Calibration (Continued) Place the display in temperature calibration mode. a. Press and hold both the UP and DOWN arrow buttons simultaneously. b. The Temperature Display will show the letters “CO”, then begin flashing the current temperature value. c. Release the arrow buttons. d. Use the Up and Down arrows individually to adjust the current temperature value until it matches the reference device temperature reading. This will correct the measurement error. e.
OPERATION (CONTINUED) Temperature Calibration Continued If the temperature readings of the incubator and the reference device still fall outside your laboratory protocol after three calibration attempts, contact Sheldon Technical Support for assistance. Note: At least three attempts may be required to sucessfully calibrate a unit that is more than 2°C out of calibration.
OPERATION (CONTINUED) MUTING THE AUDIBLE TEMPERATURE ALARM An audible and visual high or low deviation alarm will activate if the incubation chamber temperature deviates by 1°C above or below the temperature set point. The low deviation audible alarm has a delay of fifteen minutes. This prevents the low alarm from sounding whenever the doors are opened, causing a temporary drop in temperature.
OPERATION (CONTINUED) SET THE CO2 SET POINT After the temperature has been calibrated and the Over Temperature Limit set, enter the CO2 set point (concentration percentage) of your incubation application. The incubator comes from the factory with a set point of 5%. The gas supply must deliver 15 - 20 psi to the throughout this procedure Note: The visual example below represents adjusting the CO 2 set point from 4 to 5%. Press either the Up or Down arrow button on the CO2 panel. a.
OPERATION (CONTINUED) CALIBRATE THE CO2 DISPLAY Note: The incubator must be stabilized at its calibrated operational temperature and CO2 set points before the CO2 display can be accurately calibrated. The incubator must supplied with a feed of 15 – 20 psi of CO2 throughout this procedure. Always use a CO2 reference sensor regularly calibrated to a concentration accuracy of 0.1%. For best results use a digital gas analyzer with a sample collection tube.
OPERATION (CONTINUED) Calibrate the CO2 Display (Continued) Place the display into calibration mode by pressing and holding both the CO2 control Up and Down arrow buttons simultaneously. a. The letters “CO” will appear, followed by current display value, which will flash continually. b. Release the arrow buttons. c. Use the Up and Down arrows individually to adjust the flashing value so that it matches the reading of the reference CO2 analyzer. d.
OPERATION (CONTINUED) CO2 Calibration Continued If the gas CO2 concentration readings of the incubator and the reference device still fall outside your laboratory protocol after three calibration attempts, contact Sheldon Technical Support for assistance. Note: Three attempts may be required to sucessfully calibrate a unit that is more than 2% out of calibration.
OPERATION (CONTINUED) DECONTAMINATION CYCLE Note: The infrared CO2 sensor is protected from exposure to high temperatures during the decontamination cycle. It does not need to be removed at any point during normal operations, including decontamination. Note: The incubator will automatically cease injecting CO2 for the duration of the cycle.
OPERATION (CONTINUED) Decontamination Cycle (Continued) Lock the chamber doors. a. Press and hold the Operation Selector Switch on the Decontamination Cycle display sub-panel until the amber Decon Cycle indicator begins flashing. b. The doors will lock with an audible mechanical noise. c. The doors must be locked before the decontamination cycle can be initiated. Launch the decontamination cycle. a.
OPERATION (CONTINUED) Decontamination Cycle (Continued) Cool down stage. a. When the CO2 display reaches 0, the display will show the letters “CdN”. This indicates that the cycle has entered the cool down stage. b. The amber Decon Cycle and green Normal Operation indicators will flash alternatingly throughout the cool down. The cool down period will end the chamber air temperature reaches 50°C. a. The CO2 display will show the letters “PAS” when the cycle has concluded.
OPERATION (CONTINUED) ABORTING THE DECONTAMINATION AUTO CYCLE To abort the decontamination cycle perform the following steps: Press and hold both the Decontamination Cycle Switch and the Normal Operation Switch for approximately 4 seconds. a. The CO2 display will show the letters “Ab” to indicate that SCO6AD has successfully aborted the cycle. b. The Decon Activated indicator light will extinguish, though the Decon Cycle light will remain illuminated. The CO2 display will then show the letters “Cdn”. a.
OPERATION (CONTINUED) CONDENSATION AND THE DEW POINT Relative humidity inside the incubator chamber should never be allowed to exceed 95%. Exceeding this thresholds will likely result in condensation, possible leaks around the incubator, and may cause corrosion damage if allowed to continue for any significant length of time Condensation appears wherever the humidity level in the incubator chamber reaches the dew point. The dew point is the level of humidity at which the air cannot hold more water vapor.
OPERATION (CONTINUED) LOADING THE INCUBATOR Place items on the shelves inside the incubation chamber as evenly spaced as possible. Good spacing allows for maximum air circulation and a high degree of temperature uniformity. Leave 1 inch (2.5cm) between sample containers and the chamber walls. ACCESSORY COMPATIBILITY Make sure that any accessory equipment used inside the incubation chamber can safely and effectively operate within your selected temperature range, RH%, and CO 2 level.
USER MAINTENANCE Warning: Prior to any maintenance or cleaning of this unit, disconnect the power cord from the power supply. Avertissement: Avant d'effectuer toute maintenance ou entretien de cet appareil, débrancher le cordon secteur de la source d'alimentation. CLEANING AND DISINFECTING If a hazardous material or substance has spilled in the incubator, immediately initiate your site’s Hazardous Material Spill Containment protocol.
USER MAINTENANCE (CONTINUED) Disinfecting Disinfect the incubation chamber on a regular basis. For maximum effectiveness disinfection procedures are typically performed after cleaning. Perform the following steps to manually disinfect the incubator: Turn the unit off. Open all doors and carryout your laboratory, clinical, or production space disinfection protocol.
USER MAINTENANCE (CONTINUED) HEPA FILTER AND GAS LINES “IN” Sheldon Manufacturing recommends replacing the external in-line gas HEPA filter once per year, or more often when the filter is noticeably discolored. The filter is directional, and must be installed facing in the correct direction. The word “IN” is stamped on the rim of the filter assembly on the side that faces toward the gas source.
UNIT SPECIFICATIONS The SCO6AD High Heat Auto-Decontamination Incubator is a 100–120 voltage unit. Please refer to your incubator’s data plate for individual electrical specifications. Technical data specified applies to units with standard equipment at an ambient temperature of 25°C and a voltage fluctuation of ±10%.
UNIT SPECIFICATIONS (CONTINUED) CO2 Range Accuracy Recovery Time 0 – 20% ± 0.1% Less than 5 minutes Range Uniformity Stability Ambient +5ºC to 50ºC ± 0.25ºC at 37ºC ± 0.1C @ 37C TEMPERATURE POWER Model AC Voltage Amperage Frequency SCO6AD 110 – 120 12.
PARTS LIST COMPONENTS Description Parts Number Description Access Port Stopper Power Cord 125 volt 9ft 5 in (2.86m) NEMA 5-15P Brass Tubing Fitting (Gas Tubing Kit Component) Shelf Slide Door Gasket Inner Shelf Standard Door Gasket Exterior (Sold by the foot.
PARTS (CONTINUED) ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by calling 1-800-322-4897 extension 3. If you are not certain that you have the correct Part Number, or if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897 extension 4 or (503) 640-3000.
ACCESORIES Shel Lab offers the following accessories for the SCO6AD Caster Platform A rolling platform for the SCO6AD. Allows for greater ease of transportation and cleaning of floor space. Part Number 9000574 CO2 Cylinder Regulator Part Number 7150509 Compressed Gas Cylinder Switcher, Automatic Allows two gas cylinders to be connected to one gas port, and automatically switches from the first to the second cylinder when the first is empty.