System information

4 Absorbance
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4.2.1 Customizable Absorbance Paddle
Two absorbance paddles are included for customization.
The absorbance paddle can accommodate any 12.7 mm
diameter, < 6.4 mm thickness filter in the spectral range
of 400 – 800 nm. Users can order an optical filter
through a third party vendor or Turner BioSystems.
Contact Turner BioSystems’ Technical Support
Department for more details.
Wavelengths for custom filters cannot be entered in by
the user. The user has the option of selecting the custom
positions labeled A or B.
To assemble the absorbance paddle, simply place the
filter (with the mirror side down) into the opening. Press
down on the filter collar until filter fits snuggly. Avoid
getting smudges or fingerprints on the filter.
To remove the filter, turn the absorbance paddle upside
down. Press down on the collar of the filter. Avoid
touching the filter itself.
4.3 Starting a Run
Easy-to-use protocol wizards can be accessed from the HOME screen to set up the run
parameters. Follow the steps below to set up an Absorbance run.
1. From the HOME screen, touch the INSTRUMENT CONTROL button, and then the READ
button.
2. Toggle the Detection Mode to Absorbance. See Figure 4-3(A).
Figure 4-2:
Absorbance Filter
Paddle
A
B
C
D
E
F
Figure 4-3: Absorbance Parameters on the READ Screen