System information

1 Instrument
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maximum volume per well is 250 µl. Overfilling a well will result in spills and possible
damage to the instrument.
Delay: The number of seconds between the injection of the reagent and taking a
measurement. Setting a delay after the injection will allow flash-type luminescence to fully
actualize before a reading. The minimum delay value is 0.5 seconds in 0.1-second
increments.
Wavelength: Selects one or two Absorbance filters from a list.
Optical Kit: Selects a Fluorescence Optical Kit from a list.
Reading: The number of times a sample plate is read. The number of reads can be set
between 1 and 99.
Period: The time interval between readings of the same well ranging from 1 to 120
minutes in 1-minute increments.
Prime: The injector system is wetted and filled with the reagent.
Flush: The protocol used to clean the injector system after a run. The recommended
flush protocol consists of steps for washing with deionized water, 70% ethanol, deionized
water, and then air drying.
Reverse Purge: The protocol retrieves any unused reagent from the injector system.
After a run, this protocol can be used to push the reagent back into the reagent bottle to
cut down on waste.
Preset Protocols: A set of popular assay protocols preloaded into the instrument for
user convenience. These protocols cannot be renamed or deleted. A user-modified
version can be saved by touching the USER button on the PROTOCOL screen.
User Protocols: Protocols created by the user which can be retrieved later. All
parameters including the plate well mapping are saved. These protocols can be modified,
renamed, or deleted.