Instruction manual

Page 24 Leica EM CPD030 Operating Manual 09/09
5.7 Mixing the two liquid media
Press the STIRRER key. The magnetic stirrer is switched on. The magnetic stirrer
should not be used if the specimens are very delicate.
5.8 Draining media mixture from specimen chamber
Press the MEDIUM OUT key. MEDIUM OUT LED lights up.
Drain the media mixture from the specimen pressure chamber until the specimen is
barely covered by the liquid.
Close the medium outlet valve by pressing the MEDIUM OUT key again.
Press the COOLING key again. Cooling unit switches off. LED COOLING goes off.
This procedure of filling, mixing and draining needs to be repeated several times (3
minimum) before heating to remove all the acetone from the specimens. More and
larger specimens require more changes.
5.9 Heating of Specimen Pressure Chamber
Press the HEATING key. The heater installed in the specimen chamber switches
on. LED HEATING lights up.
The rising temperature of the specimen chamber is shown on temperature display.
With the rising temperature the pressure in the specimen chamber also increases
as shown on the pressure gauge.
As the liquid C02 warms up part of this liquid is transformed into gas which in turn
reduces the level of liquid.
At the time the critical temperature and the critical pressure (31qC and 73,8 bar for
CO2) have been reached, the liquid CO2 is transformed into the gaseous phase.
This is known as the critical point. The change of the physical state can be observed
through the front sight glass.
Medium
out key
Heating
key