Instruction manual

Page 22 Leica EM CPD030 Operating Manual 09/09
5.4 Insertion of Specimen into Pressure Chamber
Transfer prepared specimen into a suitable specimen container. Please contact a
Leica Mikrosysteme sales representative for more details regarding the large variety
of specimen holders available. The specimen container must be filled with acetone
prior to transfer.
Quickly insert the specimen container with the specimen into the specimen pressure
chamber filled with acetone.
If a universal specimen container, specimen transfer container or cover slip folding
basket is used, open the cover or draining mechanism after the specimen container
has been submerged in acetone.
The prepared sample must always remain hydrated until the critical point
drying process is carried out to avoid damaging the structure of the sample.
5.5 Cooling of the specimen pressure chamber
After tightly closing the screw-on cover of the specimen pressure chamber, switch
on the unit with the mains switch located on the right hand side of the operation
panel. The mains switch will illuminate green when on.
Press the COOLING key. Cooling unit starts up. LED COOLING lights up.
Actual temperature of the specimen pressure chamber is shown on the temperature
display.
At a room temperature of 21qC the specimen pressure chamber is cooled down to
10qC in about 7.5 minutes and subsequently falls below this temperature to 3q to
4qC.
The cooling unit automatically keeps the temperature of the specimen pressure
chamber below 10q C.
Cooling key
Mains
switch