Leica EM CPD030 Operating Manual Version 167060032 09/09
Important Note Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used to provide our customers with excellent products. Any copyrights of this document are retained by Leica Mikrosysteme GmbH, Vienna.
Leica EM CPD030 Operating Manual Leica EM CPD030 Serial Number: Date of purchase: For the instrument serial number, please refer to the name type label on the back of the instrument! Please read this instruction manual carefully before operating the instrument.
Tabel of contents 1. Introduction.....................................................................................................................2 1.1 2. 3. 4. 5. 6. 7. Abbreviation, notes and symbols..............................................................................3 Safety...............................................................................................................................4 2.1 Safety concept of Leica Mikrosysteme GmbH.....................................
1. Introduction Leica Mikrosysteme GmbH, Vienna is the manufacturer of the EM CPD030 critical point dryer, which is used for critical point drying of biological specimens with minimal deformation of the specimens. This operating manual enables the user to operate the EM CPD030 critical point dryer safely and in a correct manner.
1.1 Abbreviation, notes and symbols The operating manual contains abbreviations and marks to designate text sections or notes. This section explains the presentation conventions valid for the entire operating manual. Warnings and notes on use Please observe the significance of the following warnings and notes on use: Warning! Hazard or unsafe handling which may result in injuries that could be fatal. Example: Danger of electric shock when in contact with a high tension line.
2. Safety Warning! The EM CPD030 critical point dryer can be operated simply and safely, if the system is operated according to the operating manual. Hazards can however be caused by the system, if safety notes are not observed. 2.1 Safety concept of Leica Mikrosysteme GmbH The EM CPD030 critical point dryer has only few operational hazards and can be operated simply and safely by every trained user. Poorly instructed users however may cause damage and may even incur injuries through improper use.
Users and their responsibilities We designate all persons who work with or at the EM CPD030 critical point dryer as users. Because the requirements on the users vary with respect to the individual tasks we distinguish different user roles. The user may assume one or several of these user roles, if s/he meets the specified requirements. Customer The “Customer” is the contractual partner of the manufacturer and is authorized for legally binding signature of contracts.
2.2 Operational hazards and protective measures The manufacturer has minimized the consequence of existing operational hazards by design and safety measures. However, a user who is deficiently trained may cause damage or may even be injured through improper use. Pay special attention to all warnings and safety notes included in the description of activities and the following general safety regulations.
3. Description and specification Introduction Biological specimens intended for investigation in a scanning electron microscope must be dried before being introduced into the microscope (if the microscope does not have a cold stage) to allow the specimen to be imaged. If specimens such as cell suspensions are dried, tangential forces caused by molecular linkage affect the miniscule structures and cause deformation of the specimen.
Connection data: Electrical: Voltage 230 V / 240 V / 115 V Frequency 50 / 60 Hz Power consumption 220 VA Main fuse F1 / F2 4 A slow blowing (230 V / 240 V) 5 A slow blowing (115 V) Transitional fluid: Inlet M 12 x 1.5 Outlet Ø 6 mm (R1/8") Gas outlet Ø 6 mm (R1/8") Highest permissible pressure Safety bursting membrane approx.
3.
Operation and Display Panel (Overview) 1 2 3 1 2 3 Operation and display panel Specimen pressure chamber Brief operating instructions 2 1 3 11 12 13 6 5 10 7 8 4 9 14 15 16 17 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Temperature display Heating Cooling Medium In Pressure Gas out Stirrer Medium Out Stirrer Display Cooling Set Temp.
Specimen Pressure Chamber 1 2 3 1 2 3 Pressure gauge Screw-on cover for specimen pressure chamber opening View port to monitor liquid level Specimen holders or specimen baskets (see section 9) can be inserted into the specimen pressure chamber. It can be closed with a screw-on cover (1). The front sight glass (2) allows observation of the level of the transition liquid as well as the achievement of the critical point.
Cooling Unit The operating temperature of the specimen pressure chamber can be set for a range between 2qC to 12qC by pressing the SET TEMP key. The maximum permissible ambient temperature for a perfect function of the cooling unit is 30q C.
4. Unpacking, inspection and installation This chapter is directed to the service technician who installs the EM CPD030 critical point dryer and performs the initial operational set-up. The premise for this work is that the service technician is familiar with Chapter 2 and 3. 4.1 Unpacking As soon as your critical point dryer and accessories have been delivered they should be checked for damage in transit. 4.2 (1) Examine the exterior of the casing for evidence of rough handling.
4.3 Installation Required space for setting up the unit: width 490 mm, depth 660 mm. To allow adequate intake of cooling air required for the refrigeration unit and due to the inflexibility of the high pressure gas hose, the wall distance has to be at least 150 mm.
Screw the high pressure gas hose onto the MEDIUM IN connection. Connect the other end of the high pressure gas hose to the gas pressure container. The high pressure gas hose and seal are included in the accessory set of the unit. Connection of Medium Outlet Optional: Mount a PVC tube (diameter 5/10 mm) if desirable onto the pre-mounted hose nipple of the MEDIUM OUT connection. Lead other end of tube into a sealable container preferably filled with activated carbon.
Connection of Gas-Outlet The use of a flow meter (accessory) to control the dosed gas outlet from the specimen pressure chamber is highly recommended. Unscrew the pre-mounted hose nipple of the GAS OUT connection. Screw the connecting tube of the flow meter onto the GAS OUT connection. Place the flow meter on the EM CPD030 unit.
Electrical Connection Check assigned connecting voltage for the unit of the mains connection and, if necessary, adjust required connecting voltage, observing, the following procedure: Push cover (2) by inserting e.g. a screw-driver into the upper slot (3). Remove voltage selector (4) and reinsert so that the desired connecting voltage is shown at the display (1). Gently pull out the fuse holder (5) and insert fuses with the required value: 4 AT for 220 V respectively 5 AT for 115 V.
4.4 Preparing the EM CPD030 for operation Function check for the display panel Switch on main switch, on the right hand side of the front panel after connecting the power cable. Check Display Panel by pressing the DISPLAY CONTROL key. All 6 LED symbols must light up while the button is being pressed. Function check for the magnetic stirrer Insert the magnetic stirrer (included in the set of accessories) into the specimen pressure chamber and press the STIRRER key.
Setting "Lower " Nominal Temperature Press the COOLING key. The cooling unit starts up. LED COOLING lights up. The actual temperature of the specimen pressure chamber is shown on the temperature display. Press the SET TEMP key. The "lower" nominal temperature is shown on the temperature display, while the SET TEMP key is pressed. If adjustment of the lower nominal temperature is needed, turn the COOLING potentiometer located at the rear panel of the unit left or right with a small screwdriver.
5. Basic Operation This chapter is directed toward users who operate the EM CPD030 critical point dryer in normal (standard) operation. As precondition for this work, the user must be familiar with Chapter 2 and 3. 5.1 Critical Drying Method Drying water-containing biological specimens in air or under vacuum can drastically alter their structures or even destroy them completely. They must therefore be dried by a gentler method. One well-known method is "Critical Point Drying".
5.2 Preparation of Specimen Remove the specimen from the organ or the culture medium. Wash in a physiological salt solution. Fix chemically with a suitable fixing agent. Wash out fixing agent with a suitable buffer solution. Dehydrate the chemically fixed specimen with acetone or ethanol. If ethanol is used as a dehydration medium, which does not mix well with the transition liquid CO2, it must first be transformed step by step into the intermediate amylacetate or acetone.
5.4 Insertion of Specimen into Pressure Chamber Transfer prepared specimen into a suitable specimen container. Please contact a Leica Mikrosysteme sales representative for more details regarding the large variety of specimen holders available. The specimen container must be filled with acetone prior to transfer. Quickly insert the specimen container with the specimen into the specimen pressure chamber filled with acetone.
5.6 Filling C02 into the specimen chamber Wait until the specimen pressure chamber temperature has reached 10°C. Once the temperature has been reached, completely open the shut-off valve of the CO2 bottle. Shut-off valve Press the MEDIUM IN key. MEDIUM IN LED lights up. Medium In key The pressure gauge shows the pressure in the specimen chamber (identical to the pressure in the CO2 bottle). Fill the specimen pressure chamber up to the upper edge of the front sight glass with liquid CO2.
5.7 Mixing the two liquid media Press the STIRRER key. The magnetic stirrer is switched on. The magnetic stirrer should not be used if the specimens are very delicate. 5.8 Draining media mixture from specimen chamber Press the MEDIUM OUT key. MEDIUM OUT LED lights up. Medium out key Drain the media mixture from the specimen pressure chamber until the specimen is barely covered by the liquid. Close the medium outlet valve by pressing the MEDIUM OUT key again. Press the COOLING key again.
5.10 Releasing gaseous C02 from the specimen chamber After reaching the critical point, the gaseous C02 must be released from the specimen chamber. Close the gas dosing METERING VALVE. Carefully turn the valve clockwise until it stops. Do not turn the valve too tight. GAS OUT key Metering valve If the valve is closed too tightly, the valve can be damaged. Screw the connecting tube of the flow meter to the outlet GAS OUT, if this has not already been done during installation. Press the GAS OUT key.
5.11 Removing dried specimen Close the shut-off valve of the C02 bottle. Open the screw-on cover of specimen pressure chamber. Remove specimen holder with dried specimen from pressure chamber for further processing. Since the dried specimen is highly hygroscopic, it has to be coated as quickly as possible with a thin metal- or carbon film to protect it from atmospheric humidity. If this is not possible, it is recommended to keep the specimen in a desiccator until it is processed further. 5.
6. Basic Maintenance As precondition for this work, the user must be familiar with Chapter 2-5. Prior to opening to maintenance work it is crucial to close the C02 gas bottle, disconnect the gas line and disconnect the mains cable. Main switch 6.1 Screw-on Cover of Specimen Pressure Chamber Periodically check the seal of the screw on cover and replace if necessary. 6.
7. Troubleshooting Problem Cause After switching on mains switch, no function on unit is active. Disconnect power cable. Open cover of fuse compartment (rear panel of the unit – bottom left) and replace defective fuse. Fuse F 1 and/or F 2 is Make sure correct line voltage is selected! defective. Fuse values: for 220 V 4 AT for 115 V 5 AT After switching on unit and pressing key COOLING, the cooling unit doesn't start up. Fuse on power board of slide-in unit CP 010 Call service technician.
Problem Cause Remedy Shut-off valve of gas bottle is still closed. Open valve. Gas bottle without a feed pipe (riser) has been connected! Use bottle with feed pipe, otherwise only gaseous medium can be obtained. MEDIUM IN valve is open, but no transition liquid flows into C02 bottle is empty. specimen preparation chamber. MEDIUM OUT valve is open, but transition liquid does not flow out of the specimen preparation chamber.
Problem Cause Remedy Pipe connections are not screwed tightly. Level of transition liquid recedes considerably when the specimen preparation chamber is warmed up. Note: a small drop in level is normal Critical pressure is not reached when chamber is warmed up. Non return valve in inlet line is not tight due to a defective Call service technician in all cases. seal or contamination. Valve MEDIUM OUT is not tight due to a defective seal, damage on valve piston or contamination on seal surface.
EC Declaration EC Declaration of Conformity EG Konformitäts-Erklärung Déclaration CE de Conformité Leica Mikrosysteme GmbH Hernalser Hauptstrasse 219 A-1170 Wien, Austria We/Wir/Nous declare in exclusive responsibility that the product erklären in alleiniger Verantwortung, dass das Produkt déclarons sous notre seule responsabilité que le produit Model EM CPD030 Modell EM CPD030 modèle EM CPD030 Type/Typenbezeichnung/type CPD030 to which this declaration relates is in conformity with the following
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