Product Manual

BD
TM
Universal Viral Transport medium Saline solution
BD
TM
Eswab collection kit Veal Infusion Broth
Puritan Amies Transport medium Puritan UTM medium
Hank’s Balanced Salt Solution Copan UTM-RT medium
Bartel ViraTrans
TM
medium Tryptose Phosphate Broth
PBS PBS + 0.5% BSA
M4 medium M5 medium
M6 medium
FLU A & B SPECIMEN COLLECTION PROCEDURES
Good sample collection is the most important first step for an accurate test result. Therefore,
follow below instruction carefully to obtain as much secretion as possible.
Nasal Swab Specimen
Using a flocked swab provided in the OSOM Ultra Flu A&B kit, gently insert the swab approximately 1/4”
into the anterior nares (just inside the nasal orifice). Rotate the swab a few times, and repeat in the
second nostril, using the same swab.
Nasopharyngeal Swab Specimen
Using a flocked swab provided in the OSOM Ultra Flu A&B kit, insert the swab into the nostril, gently
rotating the swab inward until resistance is met at the level of the turbinates. Rotate the swab a few
times against the nasopharyngeal wall and then withdraw the swab.
Nasopharyngeal Aspirate Specimen
With the patient’s head slightly hyper-extended, instill 2.5 mL or less (the minimal volume of saline
required per patient’s size and age) of sterile saline into the patient’s nostril. Gently thread the tube
through the external nostril, into the nasopharnyx. Aspirate wash solution by gentle suction with
rotating movement.
NOTE: Catheter should remain in nasopharynx no longer than 10 seconds. Repeat the procedure until
adequate sample volume (2.5 mL) is obtained.
Nasopharyngeal Wash Specimen
Adults and Older Children
Position the patient comfortably in a sitting position, with the neck slightly hyper-extended. Prior to the
procedure, have the patient blow their nose. Using a sterile syringe, introduce 2.5 mL of sterile saline into
one nostril. If possible, have the patient retain the saline for a few seconds. Place specimen container
directly under the nose with slight pressure on the upper lip. Tilt the head forward and allow the fluid to
flow into the specimen container. Repeat the procedure on other nostril, collecting fluid into the same
container.
Infant and Younger Child
The parent should wrap one arm around the child in a manner that will restrain the child’s body and
arms. Fill a bulb syringe with 2.5 mL of sterile saline, depending on the size of the patient, and instill saline
into one nostril, while the head is tilted back. Release the pressure on the bulb to aspirate the specimen
back into the bulb. Transfer the specimen into specimen container. Repeat the procedure on other
nostril, transferring the second specimen into the same specimen container.
• If transport of the samples is required, the following transport media have been tested and shown not
to interfere with the performance of the test.