User`s manual

Section 5: Configure Menu, Part III - Calibration Coefficients
53
• Seapoint
Enter gain and offset.
Concentration = (V * 30/gain) + offset
where
Gain is dependent on cable used (see cable drawing, pins 5 and 6)
Note: SEASAVE can process data for an instrument interfacing with up to
two Seapoint fluorometers when using the New Style configuration.
• Seapoint Rhodamine (New Style configuration only)
Enter gain and offset.
Concentration = (V * 30/gain) + offset
where
Gain is dependent on cable used (see cable drawing, pins 5 and 6)
• WET Labs Flash Lamp Fluorometer (FLF) and Sea Tech
Enter scale factor and offset.
Concentration = (voltage * scale factor / 5) + offset
where
Scale factor is dependent on fluorometer range
Fluorometer Switch-Selectable Range
(milligrams/m
3
or micrograms/liter)
Scale
Factor
0 – 3 3
0 – 10 (default) 10
0 - 30 30
0-100 100
0-300 300
Sea Tech
0-1000 1000
0 – 100 100
0 – 300 (default) 300
WET Labs
FLF
0 - 1000 1000
Offset is calculated by measuring voltage output when the light sensor is
completely blocked from the strobe light with an opaque substance such
as heavy black rubber: offset = - (scale factor * voltage) / 5
• Turner 10-005
This sensor requires two channels - one for the fluorescence voltage and
the other for the range voltage. Make sure to select both when configuring
the instrument.
For the fluorescence voltage channel, enter scale factor and offset.
concentration = [fluorescence voltage * scale factor / (range * 5)] + offset
where
range is defined in the following table
Range Voltage Range
< 0.2 volts 1.0
> 0.2 volts and < 0.55 volts 3.16
> 0.55 volts and < 0.85 volts 10.0
> 0.85 volts 31.0
• Turner 10-AU-005
Enter full scale voltage, zero point concentration, and full scale
concentration from the calibration sheet.
concentration = [(1.195 * voltage * (FSC – ZPC)) / FSV] + ZPC
where
voltage = measured output voltage from fluorometer
FSV = full scale voltage; typically 5.0 volts
FSC = full scale concentration
ZPC = zero point concentration
Note:
See Application Note 54 for complete
description of calculation of Seapoint
fluorometer calibration coefficients.
Notes:
• See Application Note 9 for
complete description of calculation
of WET Labs FLF and Sea Tech
fluorometer calibration coefficients.
•
Offset and scale factor may be
adjusted to fit a linear regression of
fluorometer responses to known
chlorophyll a concentrations.