Operation Manual

7
pressing the operating button to the second
stop.
9. Recommendations for
good pipetting
- Make sure that the tip is firmly attached to
the tip cone.
- Hold the pipette vertically when aspirating
the liquid and place the tip only a few
millimeters into the liquid.
- Always control the operating button slowly
and smoothly.
- Pre-rinse the tip before aspirating the liquid
by filling and emptying the tip for three to
five times. This is important especially when
pipetting liquids with a viscosity and density
greater than water or liquids with high vapor
pressure (e.g. ethanol).
- Check that the pipette, tip and liquid are at
the same temperature.
- When pipetting liquids with temperatures
different to the ambient temperature change
the tip after each pipetting. Do not pre-rinse
the tip.
- To avoid contamination, do not rest the
pipette on its side especially with the tip
attached.
- Change the tip cone filter regularly
(recommendation after 50 - 250 pipetting
cycles).
- Never strike the tip cone against the tip tray
when mounting the tip as this can damage
the pipette.
- Avoid rough handling and do not drop the
pipette.
- Avoid exposing the unit to extreme
temperature changes, humidity and dust
(operating temperature from 15°C to 40°C).
10. Maintenance
Proline®Plus pipettes have been designed for
easy in-house service. If the pipette is used daily
it is recommended to clean/decontaminate and
check the performance of the pipette every
three months. Sartorius also provides complete
repair and recalibration service for your pipette
including a service report and performance
certificate.