Operation Manual

1. Set the volume by rotating the operating

button (clockwise to decrease the volume

and counterclockwise to increase).

NOTE: Do not rotate the operating button

outside the volume range of the pipette.

7.2. Sealing and ejecting tips

To ensure the maximum accuracy and precision,

Sartorius tips are recommended to be used with

Proline®Plus pipettes. Before fitting a tip ensure

that the pipette tip cone is clean. Press the tip on

the tip cone of the pipette.

Eject the tip by pressing the tip ejector with your

thumb. Make sure that the tip is disposed into a

suitable waste container.

7.3. Protective tip cone filters

The tip cone design of the Sartorius pipettes (>10

µl) allows the use of Safe-Cone Filters in the tip

cones. These removable filters prevent liquids

and aerosols from entering the pipette and thus,

protects the pipette from contamination and

damage.

The Safe-Cone Filters are available as Standard

or Plus versions. It is recommended to use the

Standard filter for general applications and the

Plus filter for more demanding applications such

as cell culture, bacterial and virological work and

molecular biology. Filters need to be exchanged

regularly. The interval for filter exchanging is

application dependant but the recommendation is

to change the filter daily (after 50 - 250 pipetting

cycles) and always in case of over-aspiration. (See

Chapter 2 for ordering information.) The Filter

Foreceps should be used to avoid touching the

soiled filters by hand. See Chapter 2 for ordering

information. Make sure that the filter is disposed

into a suitable waste container. Clean the tip cone

if needed and put the new filter in place.

8. Pipetting techniques

Two basic pipetting techniques, forward and

reverse pipetting, are associated with Proline®Plus

pipettes. Forward pipetting is the most common

used pipetting technique. The technique employs

Tip ejector

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