Operation Manual

12
Table 1
Z-values (µl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
NOTE: This method is based on ISO 8655.
11.2. Recalibration
The calibration of your Proline®Plus pipette
has been factory-checked by using forward
pipetting technique and certified at 22°C using
grade 3 distilled water according to ISO 3696. The
calibration is based on ISO 8655-6: Gravimetric
test method for volumetric instruments. The
pipette's specifications are guaranteed only with
genuine Sartorius tips. If you find the pipette to
be inaccurate after performance testing, please
follow the instructions below:
1. Remove the lid of the calibration nut, located
on the back of the handle, with the aid of the
calibration tool.
2. Place the hexagonal head of the calibration
tool into the hole of the calibration nut.
3. Turn the adjustment lock counterclockwise
to decrease and clockwise to increase the
volume.
4. Repeat testing the performance procedure
(Chapter 11.1.). Continue until the results
are correct.
NOTE: Recalibration is recommended if reverse
pipetting technique is used.
NOTE: Sartorius offers accredited calibration
service. Please contact your local Sartorius
representative for further information.