Operation Manual

10
lower part 360° counterclockwise. Put the pipette
into the sterilization bag and place it into the
autoclave. After autoclaving, the pipette must
be cooled down and left to dry overnight before
use. Screw the lower part of the multichannel
pipettes by holding the connecting collar and
turning the lower part 360° clockwise. Make
sure that the lower part is properly tightened.
It is recommended to check the performance
of the pipette after every autoclaving. It is also
recommended to grease the piston/seal of the
pipette after every 10 autoclaving.
11. Testing the
performance and
recalibration
It is recommended to check the performance of
your Proline®Plus pipettes regularly (e.g. every 3
months) and always after in-house maintenance.
However, the user should establish a regular
testing routine for their pipettes with regard
to accuracy requirements of the application,
frequency of use, number of operators using
the pipette, nature of the liquid dispensed and
the acceptable maximum permissible errors
established by the user. (ISO 8655-1.)
11.1. Testing the performance
Performance testing should take place in a
draught-free room at 15 - 30°C, constant to
+/- 0.5°C and humidity above 50%. The pipette,
tips and the test water should have stood in the
test room a sufficient time (at least 2 hours) to
reach equilibrium with the room conditions. Use
distilled or deionised water (grade 3, ISO 3696).
Use an analytical balance with a readability of
0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume V
s
.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel to waste
five times to reach a humidity equilibrium in
the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it
once with test water and expel to waste.
5. Aspirate the test water, immersing the tip
only 2-3 mm below the surface of the water.
Keep the pipette vertical.