Proline ® Plus pipette User Manual Bedienungsanleitung Mode d'emploi Manual Usuario Instruzioni d'impiego Инструкция пользователя
Proline® Plus pipette User Manual................................................................1 Bedienungsanleitung............................................ 15 Mode d'emploi........................................................ 31 Manual Usuario....................................................... 47 Instruzioni d'impiego............................................ 63 Инструкция пользователя........................... 79 Specifications..........................................................
Contents 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Intended use...................................................................................................... 2 Product description......................................................................................... 2 Sartorius pipette tips...................................................................................... 3 Unpacking........................................................................................................
1. Intended use Proline®Plus pipette is intended to be used in liquid handling applications for dispensing liquids in total volume range from 0,1 µl to 10 ml. It is recommended to use Optifit tips or SafetySpace™ Filter tips to ensure the best compatibility and performance with Sartorius pipettes. This liquid handling device is designed and manufactured to be used as general purpose laboratory equipment. Before operating it is recommended to read the user manual, which contains useful information e.g.
728520 Grey 1 10 10 - - 728530 Yellow 1 20 200 721014 - 728535 Yellow 1 25 200 721008 721018 728545 Yellow 1 50 200 721008 721018 728550 Yellow 1 100 200, 350 721008 721018 728560 Yellow 1 200 200, 350 721007 721017 728565 Blue 1 250 1000 721006 721016 728567 Blue 1 500 1000 721006 721016 728570 Blue 1 1000 1000 721006 721016 728575 Green 1 2000 5000 721005 721015 728580 Green 1 5000 5000 721005 721015 728590 Red 1 10 ml 10 000
4. Unpacking The Proline®Plus pipette package contains the following items: 1. 2. 3. 4. Pipette Tip Calibration tool (also tube opener) Safe-Cone filters (pipettes >10 µl and forceps) 5. Grease 6. Instruction manual 7. Performance certification in accordance with ISO 8655-6 Please check that all items are included and that no damage has occurred during shipment. 5.
– + 1. Set the volume by rotating the operating button (clockwise to decrease the volume and counterclockwise to increase). NOTE: Do not rotate the operating button outside the volume range of the pipette. 7.2. Sealing and ejecting tips To ensure the maximum accuracy and precision, Sartorius tips are recommended to be used with Proline®Plus pipettes. Before fitting a tip ensure that the pipette tip cone is clean. Press the tip on the tip cone of the pipette.
the blow-out function ensuring complete delivery of the liquid. Reverse pipetting is recommended for highly viscous, biological or foaming liquids, or very small volumes of liquid. A selected volume plus an excess is aspirated into the tip. The delivery is done without blow-out, and, thus, the excess volume remains in the tip. The reverse technique also facilitates the repeated delivery of the same volume. If reverse pipetting technique is used the pipette might need recalibration. 8.1.
pressing the operating button to the second stop. 9. - - - Recommendations for good pipetting Make sure that the tip is firmly attached to the tip cone. Hold the pipette vertically when aspirating the liquid and place the tip only a few millimeters into the liquid. Always control the operating button slowly and smoothly. Pre-rinse the tip before aspirating the liquid by filling and emptying the tip for three to five times.
NOTE: Please make sure that the pipette has been decontaminated before you send it to us or your local representative. Please advise if any hazardous material has been used with your pipette. NOTE: The use of the tip cone filters may prolong the service interval. Change the filters regularly. NOTE: It is recommended to always use gloves when cleaning the pipette. 10.1. Daily cleaning of the outer surface of the pipette Your Proline®Plus pipette should be checked every day for cleanliness.
mild detergent and soft lint-free cloth. 5. Clean the interior of the tip ejector collar and the tip cone (cylinder) with a cotton swab. Be careful with the pipettes so that the seal inside the tip cone will not damage. 6. Rinse the parts with distilled water if needed and let the parts dry. 7. Pipettes ≤ 100 µl: Put a thin layer of grease on the piston (4). Pipettes 250, 500 and 1000 µl: Put a thin layer of grease around the piston seal (4).
lower part 360° counterclockwise. Put the pipette into the sterilization bag and place it into the autoclave. After autoclaving, the pipette must be cooled down and left to dry overnight before use. Screw the lower part of the multichannel pipettes by holding the connecting collar and turning the lower part 360° clockwise. Make sure that the lower part is properly tightened. It is recommended to check the performance of the pipette after every autoclaving.
6. Withdraw the pipette vertically and touch the tip against the inside wall of the test water container. 7. Pipette the water into the weighing vessel, touching the tip against the inside wall of the vessel just above the liquid surface at an angle of 30° to 45°. Withdraw the pipette by drawing the tip 8-10 mm along the inner wall of the weighing vessel. 8. Read the weight in mgs (mi). 9. Repeat the test cycle until 10 measurements have been recorded. 10.
Table 1 Z-values (µl/mg): Temp. (°C) Air Pressure (kPa) 95 100 101.3 105 20.0 1.0028 1.0028 1.0029 1.0029 20.5 1.0029 1.0029 1.0030 1.0030 21.0 1.0030 1.0031 1.0031 1.0031 21.5 1.0031 1.0032 1.0032 1.0032 22.0 1.0032 1.0033 1.0033 1.0033 22.5 1.0033 1.0034 1.0034 1.0034 23.0 1.0034 1.0035 1.0035 1.0036 23.5 1.0036 1.0036 1.0036 1.0037 NOTE: This method is based on ISO 8655. 11.2.
12.
13. Warranty information The Proline®Plus pipettes are covered by warranty for 2 years against defects in materials and workmanship. Should your pipette fail to function at any time, please contact your local Sartorius representative.
Specifications Proline ® Plus single-, 8- and 12-channel pipettes Cat. No Channels Volume Range µl Test Volume/ Inacc. µl (%) Impr. (%) 728010 1 0.1-3 3 1.5 0.3 1.30 2.40 10.0 0.80 1.60 6.00 728020 1 0.5-10 10 5 1 1.00 1.50 2.50 0.60 1.00 1.50 728030 1 2-20 20 10 2 0.90 1.20 3.00 0.40 1.00 2.00 728040 1 5-50 50 25 5 1.00 1.40 3.00 0.30 0.50 1.50 728050 1 10-100 100 50 10 0.80 1.00 2.00 0.15 0.40 1.00 728060 1 20-200 200 100 20 0.60 0.80 2.00 0.15 0.30 0.
Sartorius Biohit Liquid Handling Oy Laippatie 1 FI-00880 Helsinki Finland Phone +358.755.951 lhinfo.finland@sartorius.com www.sartorius.com Headquarter Sartorius Corporate Administration GmbH Weender Landstrasse 94-108 37075 Goettingen, Germany Phone +49.551.308.0 Fax +49.551.308.3289 www.sartorius.com All rights reserved. No part of this publication may be reprinted or translated in any form or by any means without the prior written permission of Sartorius.
