Instructions / Assembly

DO-IT-YOURSE LF
BACTERIA IN WATER TEST
INSTRUCTIONS FOR DO-IT-YOURSELF BACTERIA IN DRINKING WATER
SAMPLING AND TESTING:
SAMPLE COLLECTION
Wash your hands before starting. Remove the test vial
with the white “tamper evident” cap.
Turn on cold water and allow it to run for approximately
five minutes before sampling. Reduce the flow of water
to about the diameter of a pencil. Remove the white cap
completely. Tilt the vial slightly and slowly fill the vial to
the 25 mL mark near the top. Do not fill past this mark.
Do not overflow the vial.
Tightly screw the cap on the vial and shake well, making
sure that the media in the vial dissolves completely.
Set the vial in an upright position and on a flat surface.
SAMPLE INCUBATION
Incubate your water sample at temperatures in the
following ranges (70°—105°F or 21°—40.6°C) indoors
or outdoors. A temperature of 95.5° F/35.3° C is best.
Test results as fast as *24 hours (*speed of test results
may vary). Test results for bacteria will take a minimum
of 24 hours (± 4 hours) to complete. Lower incubation
temperatures and/or lower bacteria numbers may cause
final results to take up to 72 hours.
Store vial in an upright position (out of direct sunlight)
until test’s result is evident.
Shaking the vial for 5–10 seconds (every 8–12 hours)
can enhance color development but is not required.
(Bend This Instructions Panel at Perforation and Carefully Remove for Use)
BONUS TEST FOR BACTERIA
SEE REVERSE SIDE FOR LAB CERTIFIED
SAMPLING AND TESTING INSTRUCTIONS
(Panel C)
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STEP #3
One (DIY) Test Vial with white “tamper
evident” cap (contains test media).
These Instructions.
CONTENTS NEEDED FOR THIS TEST
TEST RESULTS
After proper incubation time has elapsed and results
are evident, without opening the vial, observe the color
of the liquid.
Yellow = Absent (negative result for coliform bacteria).
Coliform bacteria, including E. coli bacteria are absent.
Note: As long as the sample inside the vial is clear or
yellow, allow incubation to continue for at least 72 hours,
to confirm the absence of bacteria.
Teal or Blue/Green = Present (positive result for
coliform bacteria). This test result could include E. coli.
Color and intensity may vary. NOTE: If any shade of
blue/green exists at any time during the test period
(between 24–72 hours), the test is complete.
When test is complete, open vial and discard liquid into
toilet. Place a few drops of household bleach into the vial,
cap it, shake it, and once again discard the contents into
the toilet. Recap the vial. Place the vial in a trash can.
Wash your hands thoroughly with soap and warm water.
The results of our
Safe Home® (DIY) Bacteria in Water
Test
are quite accurate. This test is a “Presence/Absence”
indicator of bacteria. If you desire additional testing,
simply go to safehometestkits.com and choose
from the variety of drinking water test kits offered on
our website.
IMPORTANT INFORMATION
DIY Test Media—Composition: a mixture of organic and
inorganic compounds. Ingredients include: Sodium Chloride,
Tryptose, Dipotassium Hydrogenorthophosphate, Potassium
dihydrogenorthophosphate, L-Tryptohan, D-Glucitol. Does not
contain any chemicals known to cause cancer, birth, or any
other reproductive defects.
Handling DIY Test Vial—Before use, contents of this
vial should be stored, tightly closed, dry, protected from light
and at a temperature between 59° F to 77° F (15° C to 25° C).
Keep away from strong oxidizing agents. Do not touch the
mouth of the vial or the inside of the cap. You will view the
test results in the comfort of your own home. Do not return
this DIY test vial to our laboratory. Once test is completed,
dispose of test vial and its contents as directed. Keep out
of reach of children.
First Aid—Do not ingest test media. If test media is inhaled,
seek fresh air. If test media makes skin contact, wash off with
plenty of water and remove contaminated clothing. If test
media makes eye contact, rinse eyes out with plenty of water.
If test media is swallowed, drink water (two glasses at most).
Consult a doctor if feeling unwell.
ABSENCE
of Harmful
Bacteria
PRESENCE
of Harmful
Bacteria