IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 1 of 51 HygroLab Bench Top Indicator version 4 Instruction Manual © 2006; Rotronic AG IN-E-HyLab-V4_11
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 2 of 51 Table of contents 1 2 2.1 2.2 2.3 2.4 2.5 3 4 4.1 4.2 4.3 4.4 4.5 5 5.1 5.2 5.3 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7 8 9 10 11 12 13 13.1 13.2 14 15 16 17 17.1 17.2 17.3 17.4 17.5 17.6 18 19 Overview ...........................................................................................................
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 3 of 51 Applicability: This manual is valid for all instruments with firmware version 4.x, where 4.x can be 4.0, 4.1, etc. (see Function Menu, SYS STATUS). Changes to the last digit of the version number reflect minor firmware changes that do not affect the manner in which the instrument should be operated.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 4 of 51 HygroLab 3: general purpose indicator with water activity functions Same as HygroLab 2, except for: - Accelerated water activity measurement with up to 4 probes (AwQuick mode): allows the measurement of most products in typically 5 minutes Full equilibration measurement with up to 4 probes (Standard mode) wi
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 5 of 51 IMPORTANT: All probes connected to the HygroLab can be supplied with 5 VDC by opening solder pad B12 and closing solder pad B13 on the main PCB. – see page 13: selection of the probe supply voltage. 2.2.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 6 of 51 have a T7 connector (not shown here). These probes can be connected to the HygroLab with the T7-03-B5 adapter cable. AW-DIO and AwVC-DIO water activity probes Direct connection to the HygroLab (probe has a B5 connector) 2.2.2 Analog probes The HygroLab can be used with one or more analog probes.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 2.4 Instruction Manual 7 of 51 Serial interface (HygroLab 2 and 3) Both the HygroLab 2 and 3 have external receptacles (located on the back panel) that provide both a RS-232 and a RS-485 interface. Serial interface settings The HygroLab indicator is compatible with the ROTRONIC HW4 software (version 1.2.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 8 of 51 1) PC with HW4 (version 1.2.2 or higher): Parity Data bits Stop bits : none :8 :1 2) PC with a third-party software using the same ASCII commands as HW3 to request data (see ASCII communications protocol).
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 9 of 51 Example of a RS-485 multi-drop In the following example, the master (address 1) is directly connected to a COM port of the PC (RS-232). Units 2 to 64 are slaves. Address 1 Address 2 Address 64 Address 3 RS232: 3 wires (incl. ground) max.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title 3 Instruction Manual Document Type Page 10 of 51 Optional configuration and communication software Depending on the model of HygroLab, the ROTRONIC HW4 software (version 1.2.2 or higher) offers additional functionality such as: - Networking (unlimited number of masters, up to 64 units per multi-drop) Instrument configuration (unit system, etc.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 11 of 51 Use of the MTA service connector is limited to configuring the instrument (unit system, output range, display, etc.). Both the HygroLab 2 and HygroLab 3 can also be configured via one of the external digital ports. Instrument configuration requires a PC with the HW4 software installed.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual 4.4 Document Type Page Document title Setting DHCP or Static Address IP Address Subnet Mask Default Gateway TCP Port MAC Address Firmware Release (device) User Name (web interface) Password (web interface) Serial Port Configuration Profile Basic Serial Settings Baud Rate Data Bits Parity Stop Bits Flow Control Instruction Manual 12 of 51 Value Static 192.168.1.1 255.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 13 of 51 - All devices within a multi-drop should use the same Baud rate. See Baud rate compatibility requirements. - Models with Ethernet (TCP/IP) interface: please note that the Baud rate of the HygroLab and the Baud rate of the internal Ethernet module should be set to the same value.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 14 of 51 The following points should also be observed: • • • • Close the parasitic circuit Connect all devices to the potential equalizing cable as often as possible. Electrical conductors such as machine elements, metal tubes or supporting constructions should be integrated into the system.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title HygroLab 1 5.1 Instruction Manual Document Type Page 15 of 51 HygroLab 2 and 3 Unit system The unit system can easily be set to Metric or English from the keypad (except HygroLab 1) -see MENU, SETTINGS. The unit system can also be changed from a PC with the optional HW4 software installed.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 16 of 51 ON/OFF: turns the instrument on or off ENTER: when the menu is active, press this key to confirm a selection MENU : press this key to activate the functions menu. The different menu items appear on the LCD message line. Press this key again to exit the menu 5.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 6.1 17 of 51 CALCULATE (HygroLab 2 and 3) Definition This function is used to select or view the humidity parameter that is calculated by the instrument. The calculated parameter is selected individually for each probe connected to the instrument 1).
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 6.2 Instruction Manual 18 of 51 MODE (HygroLab 3) Definition This function is used to set the operating mode of the HygroLab 3 Selections Standard Q AwQuick E AwE : normal operating mode (relative humidity, temperature, dew point, etc.) : water activity measurement with the AwQuick algorithm for measurements in typically 5 minutes.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 19 of 51 a) Standard Mode: No further action is required after selecting this mode. Simply proceed with the measurements. b) AwE Mode: Upon selecting this mode, the HygroLab 3 displays the following: 0. 0 0 0 1 E HS [ Aw / m i n ] Use the ▼ or ▲ arrow to set the definition of stable humidity.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 20 of 51 c) AwQuick Mode: Upon selecting this mode, the HygroLab 3 displays the following: 4 Q Dw e l l [ m i n ] Use the ▼ or ▲ arrow to set the dwell time. In the above example, the HygroLab 3 will wait 4 minutes before processing the humidity data with the AwQuick algorithm.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 6.3 Instruction Manual 21 of 51 DISPLAY (HygroLab 2 and 3) Definition This function is used to select which parameters the instrument displays. The display mode is specified globally for all probes connected to the instrument.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 22 of 51 Procedure When more than one probe is connected to the instrument, select the probe to be calibrated prior to entering the function (this is the same as the probe that was last displayed). Regarding the Adjust M.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 23 of 51 After pressing the ENTER key, the message “sure?” should appear on the LC display. Press ENTER to confirm. Next, the LC display will confirm that the probe adjustment has been successfully completed. At that time, press ENTER to exit or MENU to return to the function Adjust M.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 24 of 51 b) Humidity or Temperature 1: press the ENTER key to select. The LC display shows the value read by the probe at the time the ENTER key was pressed. The word “humidity” or “Temperature” is shown on the message line.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title 6.7 Instruction Manual Document Type Page 25 of 51 PROBE Definition This function displays the version number and serial number of any ROTRONIC HygroClip digital probe connected to the instrument. Procedure Prior to entering the function, select the probe to be displayed (this is the same as the probe that was last displayed).
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 6.9 Instruction Manual 26 of 51 SYS STATUS Definition This function displays the software version of the instrument, the RS232 configuration * (baud rate, parity, bits and stop bit), the network address *, any user defined description for the instrument * and the serial number of the instrument. This information is for display only.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 8 27 of 51 Connectors Internal service connector (HygroLab 1) Open the instrument housing to access the service connector.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 28 of 51 Optional analog output connector (HygroLab 2 and 3) If any analog probe is being used, this connector simply repeats the analog probe input signals.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title 10 Instruction Manual Document Type Page 29 of 51 Maintenance of the ROTRONIC probes Cleaning or replacing the dust filter Most ROTRONIC probes come with one of the following types of dust filter: (a) protective metal base with a removable filter cartridge or (b) plastic slotted cap with built-in (not removable) filter element.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 11 Instruction Manual 30 of 51 Specifications Power supply AC Adapter 12 VDC regulated, 100 or 300 mA 1) DC(+) Tip 0…99 %RH (non condensing) -10...55°C (14...131°F) Operating limits at electronics Measured parameters 2) relative humidity temperature barometric pressure 3) indication range indication range indication range 0.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 31 of 51 7) Accuracy, repeatability and operating limits are specified separately for each model of probe 8) ROTRONIC analog probes: because the HygroLab cannot read a negative input voltage, temperature measurement with probes having a standard output is generally limited to values above freezing.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 32 of 51 a) Both the probe and a reference thermometer should be ventilated with the same stream of air. Any dust filter used to protect the sensors should be carefully removed from the probe. If the probe has a protective slotted cap, this may be left on the probe.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 33 of 51 Instructions for using the standards • Install the calibration device on the probe so that the receptacle (or solution holder) is under the probe. Check for a tight fit and remove the receptacle from the calibration device.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 34 of 51 Water activity is the same as relative humidity except for the fact that it is expressed in p.u. instead of %RH: 100.0 %RH = 1.000 Aw.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 35 of 51 Specific humidity The specific humidity (also known as mass concentration or moisture content of moist air) is the ratio of the mass Mv of water vapor to the mass (Mv + Ma) of moist air in which the mass of water vapor Mv is contained. Q = Mv / (Mv + Ma) Q = p mw / (p mw + (Pb – p) ma) mw ma p pa Pb = 18.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 36 of 51 Enthalpy The enthalpy (or energy content) of moist air at pressure Pb, temperature t (°C) and mixing ratio r (g/kg) is defined by: h [kJ / kg moist] = 1.00464 t + 0.001846 r x t + 2.5 r Note: by convention, the enthalpy of dry air (r = 0) at 0°C is equal to zero.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 37 of 51 The accuracy of the dew point conversion is less than the accuracy that is normally achieved with a chilled mirror instrument. This is especially true at low dew point values (dew point depression of more than -40…-50°C) and at low temperatures.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 38 of 51 Data integrity 7-bit characters are sent with a parity bit. 8-bit characters are sent without parity bit. Strings (made of 7-bit characters) can be sent with or without checksum at the end of the string.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title b HygroLab 2 {b00RDD}+CR – assuming the address is 0 B HygroLab 3 {B00RDD}+CR – assuming the address is 0 R M33 series {R00RDD}+CR – assuming the address is 0 39 of 51 CR=Chr(13) Special situations (single instrument) Using address 99 in a data request causes any instrument with any address to answer.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title data request |{M00RDD} +CR Instruction Manual 40 of 51 note the | character (ASCII 124) preceding the command answer: {M00RDD 0020.41;0019.87;----.--;----.--;#E note: 0020.41: humidity probe 1 (%RH) 0019.87: temperature probe 1 (°C as per instrument configuration), etc. 17 Water activity measurement 17.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 41 of 51 Water activity is usually defined under static conditions of equilibrium. Under such conditions, the partial pressure of water vapor (p) at the surface of the product is equal to the partial pressure of water vapor in the immediate environment of the product.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 42 of 51 in the pharmaceutical industry where it provides useful information regarding the cohesion of tablets and pills, or the adherence of coatings.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title 17.3 Instruction Manual Document Type Page 43 of 51 Principle of measurement The standard (or conventional) method of measuring water activity consists in placing a sample of the product to be measured in a sealed container. The product sample slowly exchanges moisture with the air inside the sealed container until equilibrium is reached.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title 17.4 Instruction Manual Document Type Page 44 of 51 General recommendations 1. Preparation of the product samples Both the AW-DIO and the AwVC-DIO probes can be used with two different sample holders (WP14: shallow / WP-40: deep), corresponding to two sizes of disposable sample cups (PS-14: shallow / PS-40: deep).
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 45 of 51 c) Avoid warming up the probe, the sample holder or the product sample by touching or holding for too long in your hand. d) When using either the AW-DIO or the AwVC-DIO probe, check that the probe is on (this is the default when powering up the HygroLab).
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 46 of 51 When ready to measure: 1. Press on the ENTER key on the keypad of the HygroLab 3. The display shows: Note: the numerical values are for illustrative purposes only. 0. 2 9 5 A w 2 4.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document title Instruction Manual Document Type Page 47 of 51 The AwE mode runs simultaneously for all probes connected to the HygroLab. When a probe is done, the HygroLab beeps once and freezes the display for that probe. The display can be manually switched to another probe from the keypad with the ▼ or ▲ arrow.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 48 of 51 When ready to measure: 1. Press on the ENTER key on the keypad of the HygroLab 3. The display shows: Note: numerical values are for illustration purposes only. 0. 2 9 5 A w 2 4.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Instruction Manual Document Type Page Document title 49 of 51 At the end of the dwell time, the display changes as follows: 0. 9 1 5 A w 2 4. 8 1 ° C Q1 0 0 : 0 4 : 0 0R Current Aw projection average temperature AwQuick mode no longer in the dwell phase When the projected value of Aw is stable, the HygroLab beeps once and freezes the display.
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 18 Instruction Manual 50 of 51 Accessories for the HygroLab Order Code Description HW4-E HW4 software, Standard Edition ( single user) HW4-P HW4 Professional Edition, complies with ERES regulations (FDA / GAMP), multi user AC1623 Cable service connector (MTA) HygroLab 1 to PC (COM port) MOK-01-B5 Adapter cable for HygroClip
IN-E-HyLab-V4_11 Rotronic AG Bassersdorf, Switzerland Document code Unit HygroLab bench top indicator version 4: instruction manual Document Type Page Document title 19 Instruction Manual 51 of 51 Document releases Doc. Release _10 Date Dec. 6, 2006 Notes See Hardware and firmware changes release _10 _11 May 3, 2007 Made minor editorial changes Hardware and firmware changes covered in document release _10: Firmware Version 4.0 4.1a 4.1b Date Dec. 5, 2005 Sep 14, 2006 Dec 6, 2006 PCB-No.
