4411-0039-CE Version 5.
Copyright 2003 Roper Scientific, Inc. 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 609-587-9797 FAX: 609-587-1970 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Roper Scientific, Inc. Printed in the United States of America. IPLab is a trademark of Scanalytics, Inc. Macintosh is a registered trademark of Apple Computer, Inc. Microsoft, Windows, and Windows NT are registered trademarks of Microsoft Corporation.
Table of Contents Chapter 1 Introduction .......................................................................................11 Introduction....................................................................................................................... 11 MicroMAX System Components ..................................................................................... 11 Overview ....................................................................................................................
iv MicroMAX System User Manual Version 5.A Cooling ............................................................................................................................. 33 Setting the Temperature ............................................................................................. 34 Temperature Stabilization .......................................................................................... 34 Baseline Signal ........................................................................
Table of Contents v Timing Modes................................................................................................................... 76 Free Run ..................................................................................................................... 76 IEC (Internal Exposure Control) ................................................................................ 78 EEC (External Exposure Control)..............................................................................
vi MicroMAX System User Manual Version 5.A Inputs .............................................................................................................................. 109 Outputs............................................................................................................................ 109 Programmable Interface.................................................................................................. 109 A/D Converter .....................................................
Table of Contents vii Limited One (1) Year Warranty on Refurbished or Discontinued Products ............ 147 Normal Wear Item Disclaimer.................................................................................. 147 VersArray (XP) Vacuum Chamber Limited Lifetime Warranty.............................. 148 Sealed Chamber Integrity Limited 24 Month Warranty........................................... 148 Vacuum Integrity Limited 24 Month Warranty .......................................................
viii MicroMAX System User Manual Version 5.A Figure 36. Setup using to Trigger an Event....................................................... 77 Figure 37. Timing for Experiment Setup shown in Figure 36......................................... 77 Figure 38. Timing Diagram for Typical IEC Measurement ............................................ 79 Figure 39. Setup for IEC Experiment with Two Lasers .................................................. 79 Figure 40.
Table of Contents ix Table 10. Bit Values with Decimal Equivalents: 1 = High, 0 = Low............................. 86 Table 11. TTL In/Out Connector Pinout ......................................................................... 87 Table 12. ST-133A Shutter Drive Selection.................................................................... 95 Table 13. I/O Address & Interrupt Assignments before Installing Serial Card............. 103 Table 14.
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Chapter 1 Introduction Introduction The Princeton Instruments MicroMAX system is a high-speed, low-noise CCD camera system designed for demanding imaging applications and is an optimal system for use in fluorescence microscopy applications such as high-resolution immunofluorescence, FISH or GFP imaging. The MicroMAX system incorporates a compact camera head, cooled CCD, advanced exposure-control timing, video output, and sophisticated readout capabilities.
MicroMAX System User Manual Version 5.A Camera Introduction: The function of the camera is to collect very low intensity light and convert the energy into a quantitative, electronic signal (photo-electrons) over a two dimensional space. To do this, light from the subject is focused onto a CCD array, which accumulates photoelectrons for the exposure time. At the end of the exposure time, the image thus formed is read out.
Chapter 1 Introduction 13 Controller Data Conversion: The controller accepts the analog data and converts it to digital data using specially designed, low-noise electronics supporting scientific grade 12- or 16-bit Analog to Digital (A/D) converters. The standard MicroMAX Controller enables both high-speed and high-precision readout capabilities.
MicroMAX System User Manual Version 5.A Operating System: Windows® 95/ME/2000/XP or Windows NT® Interface: PCI High-Speed Serial I/O card. Computers purchased from Roper Scientific as part of the MicroMAX system are shipped with the card installed. Computer Monitor: Super VGA monitor with 256 color graphics card and at least 512 kbytes of memory. Mouse: Two-button Microsoft®-compatible serial mouse or Logitech three-button serial/bus mouse.
Chapter 1 Introduction 15 Appendix B, Outline Drawings includes outline drawings of the MicroMAX cameras and the ST-133A Controller. Appendix C, Kinetics Mode describes how to set up and acquire data with the Kinetics option, which allows frame transfer CCDs to take time-resolved images/spectra. Appendix D, Virtual Chip Mode describes how to set up and use the Virtual Chip option, a special fast-acquisition technique.
MicroMAX System User Manual Version 5.A Grounding and Safety The apparatus described in this manual is of the Class I category as defined in IEC Publication 348 (Safety Requirements for Electronic Measuring Apparatus). It is designed for indoor operation only. Before turning on the controller, the ground prong of the power cord plug must be properly connected to the ground connector of the wall outlet.
Chapter 1 Introduction 17 Cleaning WARNING! Turn off all power to the equipment and secure all covers before cleaning the units. Otherwise, damage to the equipment or personal injury could occur. Camera and Controller Although there is no periodic maintenance that must be performed on the camera or the ST-133A Controller, you may clean these components from time to time by wiping them down with a clean damp cloth. This operation should only be done on the external surfaces and with all covers secured.
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Chapter 2 Installation Overview The list and diagrams below briefly describe the sequence of actions required to hookup your system and prepare to gather data. Refer to the indicated references for more detailed information. This list assumes that the application software is Princeton Instruments WinView/32. Action Reference 1. If the system components have not already been unpacked, unpack them and inspect their carton(s) and the system components for intransit damage. Store the packing materials.
MicroMAX System User Manual Version 5.A Action Reference 11. If using a microscope Xenon or an Hg arc lamp, turn it on before turning on the controller and host computer. Chapter 4 Operation, page 33 12. Turn the Controller ON. 13. Turn on the computer and begin running WinView/32. WinView/32 manual 14. Enter the hardware setup information or load the defaults from the controller. Chapter 4 Operation, page 39 15. Set the target array temperature. Chapter 4 Operation, page 33 16.
Chapter 3 System Setup Unpacking the System During the unpacking, check the system components for possible signs of shipping damage. If there are any, notify Roper Scientific and file a claim with the carrier. If damage is not apparent but camera or controller specifications cannot be achieved, internal damage may have occurred in shipment. Please save the original packing materials so you can safely ship the camera system to another location or return it to Roper Scientific for repairs if necessary.
MicroMAX System User Manual Version 5.A Power Requirements The MicroMAX system can operate from any one of four different nominal line voltages, 100, 120, 220, or 240 V AC. The power consumption is nominally 200 watts and the line frequency can range from 47 to 63 Hz. The MicroMAX camera receives its power from the controller, which in turn plugs into a source of AC power.
Chapter 3 System Setup 23 Mounting the Camera General The MicroMAX camera can be mounted either horizontally or vertically (nose up or nose down). The camera can rest on any secure surface. For mounting flexibility, the round head camera is equipped with four standard ¼″ x 20 UNC threaded 3/8″ deep holes spaced at 90° intervals around the body; in some situations it may prove convenient to secure the camera with a suitable mounting bracket.
MicroMAX System User Manual Version 5.A press the locking lever on the mount while rotating the lens clockwise until it comes free and can be pulled straight out. Both types of lenses typically have provision for focusing and aperture adjustment, with the details varying according the make and model of the lens. In addition, in the case of the F-mount, there is provision for adjusting the focus of the lens mount itself, if necessary, to bring the focus within range of the lens focus.
Chapter 3 System Setup 25 C-Mount For a camera equipped with a C-mount thread, use the standard C-mount adapter supplied by the microscope manufacturer to attach the camera to the microscope. The adapter can be screwed into the camera and then the assembly can be secured to the microscope using the standard setscrews on the microscope. The camera can be mounted on the trinocular output port, the side port, or the bottom port of the inverted microscope.
MicroMAX System User Manual Version 5.A Diagnostic Instruments Bottom Clamp Type Microscope Type Leica DMR L-clamp Leitz All types NLW-clamp Nikon Optiphot, Diaphot, Eclipse O-clamp Olympus BH-2, B-MAX, IMT-2 V-clamp Zeiss Axioscope, Axioplan, Axioplan 2, Axiophot Z-clamp Zeiss Axiovert ZN-clamp Table 1. Bottom Clamps for Different Microscopes The F-mount is appropriate for any trinocular output port or any side port.
Chapter 3 System Setup 27 1X HRP 100-NIK "L" bottom clamp Figure 6. Bottom Clamp secured to Relay Lens Caution Microscope optics have very high transmission efficiencies in the infrared region of the spectrum. Since typical microscope light sources are very good emitters in the infrared, some microscopes are equipped with IR blockers or heat filters to prevent heating of optical elements or the sample.
MicroMAX System User Manual Version 5.A Installing the Application Software Installation is performed via the WinView/32 installation process, which should be done before the interface card is installed in the host computer. On the Select Components dialog box (see Figure 7), click on the button appropriate for the interface card. For a PCI card, select the AUTO PCI component to install the required PCI card driver and the most commonly installed program files.
Chapter 3 System Setup 29 To Install an ISA Serial Card: Support for ISA Serial boards has been discontinued as of the release of WinView/32 version 2.6.0. Earlier versions of the software still support this board. If you are using an earlier version of the WinView software and want to install an ISA card, contact the factory for instructions. Note: An ISA serial interface card operated in an ISA slot can support data transfer rates as high as 1 MHz (WinView software ver. 1.4.3 - 2.4.8).
MicroMAX System User Manual Version 5.A Selecting the Shutter Setting Caution The Shutter Setting push switch on the rear of the Controller sets the shutter hold voltage. Each shutter type, internal or external, requires a different setting. Consult the table below for the proper setting for your shutter. The Shutter Setting is correctly set at the factory for the camera’s internal shutter if one is present.
Chapter 3 System Setup 31 Connecting the Detector-Controller Cable or the Camera Power/Camera Signal Cables Caution Turn the Controller power OFF (OFF = 0, ON = |) before connecting or disconnecting the Detector-Controller cable or the Camera Power/Camera Signal Cables. To Connect the Detector-Controller Cable: 1. Verify that the Controller power is OFF. 2. Connect male end of the Detector-Controller cable to the “Detector” port on the back of the Controller. 3.
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Chapter 4 Operation Introduction This chapter begins with sections regarding a number of operating considerations such as EMF, vacuum, cooling, baseline signal, and imaging field of view. The final section provides a step-by-step procedure for placing the system in operation the first time. At this point a lens should be mounted on the camera (or, if necessary, the camera mounted on a microscope) and you should be ready to operate the system and proceed to viewing your first MicroMAX images.
MicroMAX System User Manual Version 5.A enclosure of the camera. Fins on the round head camera shell radiate the heat outward to the surrounding atmosphere. The fan inside the rectangular head camera draws air through the vents in the camera shell, blows it through the internal fins, and exhausts it back into the atmosphere through the vents.
Chapter 4 Operation 35 Imaging Field of View When used for two-dimensional imaging applications, the MicroMAX camera closely imitates a standard 35 mm camera. Since the CCD is not the same size as the film plane of a 35 mm camera, the field of view at a given distance is somewhat different. The imaging field of view is indicated in Figure 9. CCD Object Lens S O B D Figure 9. Imaging Field of View D = distance between the object and the CCD B = 46.5 mm for F-mount; 17.
MicroMAX System User Manual Version 5.A Note: If more than one device is connected to the video output, the last device is the one that should to be terminated in 75Ω. For example, to connect the video output to a VCR as well as to a monitor, the cable from the controller video output should be connected to the video input connector of the VCR, and another 75 Ω cable should extend from the video output connector of the VCR to the 75Ω input of the monitor.
Chapter 4 Operation 37 Note: With a 16-bit A/D converter (not a standard option), the composite video output is disabled during data acquisition. In post-acquisition processing the WinView/32 ROI (Region of Interest) capability allows any portion of an acquired image to be displayed on the computer monitor. Again, note that the described video output behavior applies specifically for the WinView/32 software only. Other application software may provide different video output capabilities.
MicroMAX System User Manual Camera-Controller Cable Assy. Version 5.A TAXI cable (Serial Com) 110/220 Camera Camera Camera Serial Pwr Signal 110/220 Controller Microscope Computer EXPERIMENT Figure 12. 5 MHz System Diagram Assumptions The following procedure assumes that 1. You have already set up your system in accordance with the instructions in Chapter 3. 2. You have read the previous sections of this chapter. 3. You are familiar with the application software. 4. The system is air-cooled. 5.
Chapter 4 Operation 39 described in Chapter 3. Be sure to secure both ends of the cable with the cableconnector screws. • Connect a 75 Ω BNC cable from the VIDEO connector on the back of the camera to the video monitor’s 75 Ω input. This cable must be terminated in 75 Ω. Many monitors have a switch for selecting the 75 Ω termination. • Connect the line cord from the Power Input assembly on the back of the controller to a suitable source of AC power. Getting Started 1.
MicroMAX System User Manual Version 5.A Detector Temperature (Setup|Detector Temperature…): -15°C for round camera heads or -45°C for rectangular camera heads. The temperature should drop steadily, reaching the set temperature in about ten minutes (typical). At that point the green Temp Lock LED on the rear of the ST-133A will light and there will be a locked indication at the computer monitor, indicating that temperature lock has been established. Note that some overshoot may occur.
Chapter 4 Operation 41 Note: If you are using WinView/32 and a video monitor for focusing, select the Video Focus… mode from the Acquisition menu. Then select a short exposure time (0.1 s), an Intensity Scaling setting of 4096, and 2x Zoom. With an MicroMAX:1300Y camera (1030×1300 pixels), set the Pan selector as required for the 756×486 subset of the array image you wish to use for focusing purposes.
MicroMAX System User Manual Version 5.A setscrews as shown in Figure 13. To change the focus setting, proceed as follows. o Loosen the setscrews with a 0.050" Allen wrench. Do not remove the screws; loosen them just enough to allow the lens mount to be adjusted. o Rotate the lens mount as required to bring the focus within range of the lens focus adjustment. o Tighten the setscrews loosened above.
Chapter 4 Operation 43 it is possible to use a broadband source such as tungsten for the alignment. If this is the case, use a wavelength setting of 0.0 nm for alignment purposes. Assumptions The following procedure assumes that 1. You have already set up your system in accordance with the instructions in Chapter 3. 2. You have read the previous sections of this chapter. 3. You are familiar with the application software. 4. The system is air-cooled. 5. The system is being operated in spectroscopy mode.
MicroMAX System User Manual • Controller type: ST-133 • Controller version: 3 or higher • • Camera type: Select the array installed in your detector. MicroMAX:512BFT = EEV 512×512 FT CCD57 MicroMAX:782Y = PID 582×782 MicroMAX:782YHS = PID 582×782 MicroMAX:1024B = EEV 1024×1024 CCD47_10 MicroMAX:1300Y = PID 1030×1300 MicroMAX:1300YHS = PID 1030×1300 MicroMAX:1300YHS-DIF = PID 1030×1300 Shutter type: None or Remote. • Readout mode: Full frame. Version 5.
Chapter 4 Operation 45 of focus and adjust for optimum while watching a live display on the monitor, followed by rotating the detector and again adjusting for optimum. The following procedure, which describes the focusing operation with an Acton 300I spectrograph, can be easily adapted to other spectrographs. 1. Mount a light source such as a mercury pen-ray type in front of the entrance slit of the spectrograph. Any light source with line output can be used.
MicroMAX System User Manual Version 5.A Acquiring Data Once optimum focus and aperture have been achieved, you can switch from Focus (or Video Focus) mode to standard data-acquisition operation as determined via the Experiment Setup dialog box. (In WinSpec/32, you might want to begin with Free Run Asynchronous (Safe Mode) operation while gaining basic system familiarity.) This completes First Light for spectroscopy applications.
Chapter 5 Timing Modes The Princeton Instruments MicroMAX system has been designed to allow the greatest possible flexibility when synchronizing data collection with an experiment. The chart below lists the timing mode combinations. Use this chart in combination with the detailed descriptions in this chapter to determine the optimal timing configuration. Mode Shutter Free Run Normal External Sync Normal External Sync PreOpen Continuous Cleans Normal Continuous Cleans PreOpen Table 4.
MicroMAX System User Manual Version 5.A sends the Stop Acquisition command to the camera, instructing it to stop acquisition. Once that frame is completely processed and displayed, another Start Acquisition command is sent from the computer to the camera, allowing it to take the next frame. Display is therefore, at most, only one frame behind the actual data collection.
Chapter 5 Timing Modes 49 Safe Mode (Asynchronous) Full Speed Mode (Synchronous) Start Start Computer programs camera with exposure and binning parameters Computer programs camera with exposure and binning parameters STARTACQ issued from computer to camera STARTACQ issued from computer to camera Cleans performed Cleans performed 1 frame collected as per timing mode 1 frame collected as per timing mode STOPACQ issued from computer to camera Background or flatfield on? No Yes Background or f
MicroMAX System User Manual Version 5.A Shutter opens Shutter remains open for preprogrammed exposure time System waits while shutter closes Figure 15. Free Run Timing Chart (part of the chart in Figure 14) Other experimental equipment can be synchronized to the MicroMAX system by using the (NOTSCAN) signal. This TTL output for synchronous operation is shown in Figure 16.
Chapter 5 Timing Modes 51 In the PreOpen Shutter mode, on the other hand, shutter operation is only partially synchronized to the experiment. As soon as the controller is ready to collect data the shutter opens. Upon arrival of the first External Sync pulse at the MicroMAX, the shutter remains open for the specified exposure period, closes, and the CCD is read out. As soon as readout is complete the shutter reopens and waits for the next frame.
MicroMAX System User Manual Version 5.A Also note that, in addition to signal from ambient light, dark charge accumulates during the “wait” time (tw). Any variation in the external sync frequency also affects the amount of dark charge, even if light is not falling on the CCD during this time. Note: If EXT SYNC is still active at the end of the readout, the hardware will interpret this as a second sync pulse, and so on.
Chapter 5 Timing Modes 53 Note: If EXT SYNC is still active at the end of the readout, the hardware will interpret this as a second sync pulse, and so on. Open Shutter (Normal) Shutter (Preopen) Open Scan Close Close Read Open Open Close Close Read Open Open Close Close Read External Sync Figure 20. Continuous Cleans Timing Diagram Frame Transfer Operation In frame transfer operation, half the CCD is used for sensing light and the other half for storage and readout.
MicroMAX System User Manual Version 5.A low). More specifically, if the readout time, tR, is greater than the sum of tw1, the time the controller waits for the first External Sync pulse, plus texp, the programmed exposure time, plus tc, the shutter compensation time, then the actual exposure time will equal tR. If an External Sync pulse is detected during each read, frames will follow one another as rapidly as possible as shown in Figure 21.
Chapter 5 Timing Modes 55 texp Shutter Monitor actual exposure time tR tR Scan tR tR External Sync (negative polarity shown) tw1 tR tc cleans acquisition Figure 22. Frame Transfer where tw1 + texp + tc > tR texp Shutter Monitor actual exposure time tR tR Scan tR tR External Sync (negative polarity shown) cleans acquisition tw1 tc Figure 23.
MicroMAX System User Manual • Version 5.A Non-overlapped: This operation mode is automatically selected by the controlling software when the exposure time is less than the readout time. In non-overlapped operation, the image is transferred to the storage cells at the end of the exposure time and no further accumulation occurs (the imaging cells are switched off).
Chapter 5 Timing Modes 57 counting of the programmed Exposure Time begins when the sync pulse arrives at the Ext Sync connector. The exposure ends on completion of the programmed Exposure Time. Then the data acquired during the first exposure is read out while the next frame of data is being acquired. This pattern continues for the duration of the experiment so that, during each frame, the data acquired during the previous frame is read out. texp Shutter Monitor actual exposure time 50ns min.
MicroMAX System User Manual Version 5.A texp Shutter Monitor actual exposure time Scan tR tR tR External Sync (negative polarity shown) cleans acquisition tw1 tc Figure 26.
Chapter 6 Exposure and Readout Before each image from the CCD array appears on the computer screen, it must first be read, digitized, and transferred to the computer. Figure 27 is a block diagram of the image-signal path. Incoming photons Controller Camera Up/down integrator CCD Slow A/D Fast A/D Preamp Digital processor Video display Cable driver HS serial interface HS serial buffer board Display Storage Computer Figure 27.
MicroMAX System User Manual Version 5.A CCD arrays perform three essential functions: photons are transduced to electrons, integrated and stored, and finally read out. CCDs are very compact and rugged. Unintensified, uncoated CCDs can withstand direct exposure to relatively high light levels, magnetic fields and RF radiation. They are easily cooled and can be precisely temperature controlled to within a few tens of millidegrees.
Chapter 6 Exposure and Readout Mechanical Shutter Scan 61 Open Closed Acquire texp Exposure time Readout tc Shutter compensation Figure 28. CCD Exposure with Shutter Compensation is low during readout, high during exposure, and high during shutter compensation time. Since most shutters behave like an iris, the opening and closing of the shutter will cause the center of the CCD to be exposed slightly longer than the edges.
MicroMAX System User Manual Version 5.A Interline For interline CCDs, image smearing may occur due to a small amount of light leaking through to the storage cells during the readout time. In the case of lens-coupled intensified cameras (ICCDs), this effect can be eliminated by using a fast phosphor and gating the intensifier at the same frame rate as the CCD.
Chapter 6 Exposure and Readout 63 Array Readout In this section, a simple 6 × 4 pixel CCD is used to demonstrate how charge is shifted and digitized. As described below, two different types of readout are available. Full frame readout, for full frame CCDs, reads out the entire CCD surface at the same time. Frame transfer operation assumes half of the CCD is for data collection and half of the array is a temporary storage area.
MicroMAX System User Manual Version 5.A digitized is the second column moved into the shift register. The order of shifting in our example is therefore D6, C6, B6, A6, D5, C5, B5, A5, D4.... After charge is shifted out of each pixel the remaining charge is zero, meaning that the array is immediately ready for the next exposure. Below are the equations that determine the rate at which the CCD is read out. Tables of values for CCDs supported at the time of the printing of this manual also appear below.
Chapter 6 Exposure and Readout 65 Frame Transfer The MicroMAX fully supports frame transfer readout. Operation in this mode is very similar to the operation of video rate cameras. Half of the CCD is exposed continuously, raising the exposure duty cycle to nearly 100%. The other half of the CCD is masked to prevent exposure, and it is here that the image is “stored” until it can be read out. Figure 30 shows the readout of a masked version of our sample 4 × 6 CCD.
MicroMAX System User Manual Version 5.A Interline In this section, a simple 6 × 3 pixel interline CCD is used to demonstrate how charge is shifted and digitized. As described below, two different types of readout, overlapped and non-overlapped can occur. In overlapped operation, each exposure begins while the readout of the previous one is still in progress.
Chapter 6 Exposure and Readout 1 Empty Readout Register. Exposure has ended and image is being transferred to storage cells. 2 Image has been shifted to storage cells, first line has been shifted to Readout Register, and second exposure begins.
MicroMAX System User Manual Version 5.A Part 4 of Figure 32 illustrates the situation at the end of the readout. Both the imaging and storage cells are empty. In Free Run operation, the imaging cells will be switched back on immediately, allowing charge accumulation to begin. In Ext Sync operation with no PreOpen, they are not switched back on until after the External Sync pulse is detected. 1 Empty Readout Register. Exposure has ended and image is being transferred to storage cells.
Chapter 6 Exposure and Readout 69 where Nx is the smaller dimension of the CCD Ny is the larger dimension of the CCD. tsr is the time needed to shift one pixel out of the shift register tv is the time needed to digitize a pixel ti is the time needed to shift one line into the shift register CCD Array 1 MHz Readout 5 MHz Readout MicroMAX:782Y Sony ICX075 782 x 582 0.5 sec. for full frame N/A MicroMAX:782YHS Sony ICX075 782 x 582 N/A 0.11 sec.
MicroMAX System User Manual Version 5.A render the camera photon shot noise limited, the S/N ratio improvement is roughly proportional to the square-root of the number of pixels binned. Figure 33 shows an example of 2 × 2 binning for a full frame CCD array. Each pixel of the image displayed by the software represents 4 pixels of the array. Rectangular bins of any size are possible. 1 Empty Readout Register. Exposure has ended and image is about to be shifted into the Readout Register.
Chapter 6 Exposure and Readout 71 On-Chip Binning for Interline Binning is the process of adding the data from adjacent cells together), and it can be accomplished in either hardware or software. Rectangular groups of cells of any size may be binned together, subject to some hardware and software limitations. Hardware binning is performed before the signal is read out by the preamplifier.
MicroMAX System User Manual Version 5.A Software Binning One limitation of hardware binning is that the shift register pixels and the output node are typically only 2-3 times the size of imaging pixels as shown in Table 9. Consequently, if the total charge binned together exceeds the capacity of the shift register or output node, the data will be corrupted.
Chapter 6 Exposure and Readout always be nosier than one designed for optimum noise performance. The MicroMAX camera can be provided with two analog converters, one optimized for high speed, the other for high precision, as a solution to this problem. For the most common system configurations, there will be a 1 MHz converter for the fastest possible data collection, and a 100 kHz converter for use where noise performance is the paramount concern.
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Chapter 7 MicroMAX DIF Camera (Double Image Feature) Introduction This Appendix describes operation of the MicroMAX DIF system. Both the Controller and a MicroMAX Interline camera must have factory modifications installed for DIF operation. In addition to the internal changes, a camera modified for DIF operation would ordinarily include a mechanical shutter. Execution of the DIF functions is done via the WinView/32 software (v2.
MicroMAX System User Manual Version 5.A ESABI: Allows two fast images of equal duration to be acquired. Unlike the IEC and EEC modes, in the ESABI mode, two pulses are applied to the Ext. Sync. connector. Each initiates a separate acquisition, allowing the user to set the time between acquisitions by externally adjusting the time between the two applied pulses. When the data is saved, both images are saved in a single *.spe file.
Chapter 7 MicroMAX DIF Camera 77 image. As soon as the first exposure actually begins, Figure 35. Thus the positive going edge of the exposure. In Free Run operation, the time that the range of 400 to 600 ns. returns high, as shown in output marks the start of the first remains low will typically be in READY 400 ns EXPOSURE Figure 35.
MicroMAX System User Manual Version 5.A Summary of Free Run Timing mode • Allows user to capture single images. • Requires that the switch, if present on the back of the camera, be set to INACTIVE. • Uses Exposure Time set via software Experiment Setup. • Exposure time range is 1 µs < Exp. Time < 14.3 minutes • • • Exposure does not occur until the mechanical shutter is completely open and readout does not begin until the mechanical shutter is completely closed.
Chapter 7 MicroMAX DIF Camera 79 200 ns EXT. SYNC. READY 1st Image 5 µs 2nd Image Mechanical Shutter 5 µs 8 ms 8 ms Figure 38. Timing Diagram for Typical IEC Measurement Figure 39 illustrates the interconnections that might be used for such an experiment with two lasers. Figure 40 shows the timing for the two-laser experiment. Computer Controller READY DG-535 A B C EXT SYNC DG-535 can run at a fairly slow rep rate or use READY signal as a trigger.
MicroMAX System User Manual Version 5.A Example 2: As shown in Figure 41, the signal from the controller can be used to trigger the controller by connecting it back into the EXT SYNC connector. At the same time, it can be used to trigger a DG-535. Computer EXT SYNC READY Controller DG-535 Ext. A B Laser 1 Camera Head Laser 2 Figure 41. Another Hardware Setup for an IEC Measurement Note: This setup will not work in the EEC mode or the ESABI mode.
Chapter 7 MicroMAX DIF Camera 81 supplied by the user is required to initiate the imaging process and control the first image exposure time. The controller signal goes low when the camera is ready to begin imaging. Figure 42 illustrates an EEC timing example. READY EXT. SYNC. (A) 1st Image 2nd Image t exp Figure 42. EEC Timing Example with Exposure Time in Software Set to texp Summary of EEC Timing mode • Enables double image capture under external control.
MicroMAX System User Manual Version 5.A READY EXT. SYNC. (A) 1st Image t trig t exp No Signal Integration 2nd Image t exp Figure 43. ESABI Timing Example: Image Exposure time = texp set in software Note: The input trigger pulse, ttrig, must be shorter than the exposure time texp. Otherwise the second image will occur immediately after the first. Summary of ESABI Timing mode • • • The exposure time selected in Experiment Setup sets the exposure time of both the first and second image.
Chapter 7 MicroMAX DIF Camera 83 controller as well as time-dependent (but constant for a fixed integration time) buildup of dark charge. The background subtract equation is: (Raw image data – Background) = Corrected image data. When background and flatfield operations are both performed, background subtraction is always performed first. Flatfield Correction Flatfield correction allows the user to divide out small nonuniformities in gain from pixel to pixel.
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Chapter 8 TTL Control Introduction This connector provides 8 TTL lines in, 8 TTL lines out and an input control line. Figure 44 illustrates the connector and Table 11 lists the signal/pin assignments. Princeton Instruments WinView/32 software packages incorporate WinX32 Automation, a programming language that can be used to automate performing a variety of data acquisition and data processing functions, including use of the TTL IN/OUT functions.
MicroMAX System User Manual Version 5.A Table 10 illustrates this coding for decimal values 0 through 7. Obviously this table could easily be extended to show the coding for values all the way to 255. Decimal Equiv.
Chapter 8 TTL Control Pin # Assignment Pin # 87 Assignment 1 IN 1 14 IN 2 2 IN 3 15 IN 4 3 IN 5 16 IN 6 4 IN 7 17 IN 8 5 GND 18 GND 6 EN/CLK 19 Reserved 7 (future use) 20 GND 8 GND 21 OUT 2 9 OUT 1 22 OUT 4 10 OUT 3 23 OUT 6 11 OUT 5 24 OUT 8 12 OUT 7 25 GND 13 Reserved Table 11. TTL In/Out Connector Pinout Figure 44.
MicroMAX System User Manual Version 5.A • RG/58U coaxial cable. • Shielded Metalized hood (Radio Shack part no 276-1536A). • BNC connector(s) type UG-88 Male BNC connector (Radio Shack part no 278-103). Example Suppose you needed to build a cable to monitor the line TTL OUT 1. One approach would be to build a cable assembly as described in the following paragraphs. This procedure could easily be adapted to other situations. 1.
Chapter 9 System Component Descriptions MicroMAX Camera CCD Array: MicroMAX offers a choice of CCD technologies to improve quantum efficiency (QE) and blue/green sensitivity. Arrays are available in full-frame, interline, and frame-transfer formats. Thinned, back- illuminated devices have a higher QE across the entire visible spectrum and far superior sensitivity in the blue/ green region than front-illuminated CCDs.
MicroMAX System User Manual Version 5.A forced air. CCD temperature is controlled and monitored by via the host computer and the ST-133A Controller. Shutter: Rectangular head cameras are available with an internal 25 mm shutter. A shutter drive signal is available at the Remote shutter connector on the rear of the ST-133A Controller or on the rear of the camera. Electronics: The camera electronics enclosure contains the preamplifier and array driver board.
Chapter 9 System Component Descriptions 91 drawn into the camera by the internal fan and exhausted through the back panel. The fan is always in operation and air cooling of both the Peltier and the internal electronics takes place continuously. The fan is designed for low-vibration and does not adversely affect the image. For the fan to function properly, free circulation must be maintained between the rear of the camera and the laboratory atmosphere.
MicroMAX System User Manual Version 5.A ST-133A Controller Electronics: The Model ST-133A is a compact, high performance CCD Camera Controller for operation with Princeton Instruments cameras. Designed for high speed and high performance image acquisition, the ST-133A offers data transfer at speeds up to 5 megapixel per second, standard video output for focusing and alignment. A variety of A/D converters are available to meet different speed and resolution requirements.
Chapter 9 System Component Descriptions 93 The Analog/Control Module, which should always be located in the left-most slot, provides the following functions. • Pixel A/D conversion • Timing and synchronization of readouts • CCD scan control • Temperature control • Exposure control • Video output control The Interface Control Module, which should always be located in the center slot, provides the following functions.
MicroMAX System User Manual Version 5.A The descriptions of the rear panel connectors that follow are keyed to Figure 46. # Feature 1. Temperature Lock LED: Indicates that the temperature control loop has locked and that the temperature of the CCD array will be stable to within ± 0.05°C. 2. Video Output: Composite video output is provided at this connector. The amplitude is 1 V pk-pk and the source impedance is 75 Ω.
Chapter 9 System Component Descriptions # 95 Feature 12. Camera Pwr: (5 MHz system) Provides the power to operate the camera. The power cable must be connected before the Camera Signal cable is connected. 13. Fan: Cools the controller electronics. Runs continuously when the controller is turned on. Do not block the side vents or the fan exhaust port. • Shutter Setting Selector: Sets the shutter hold voltage. Dial is correctly set at the factory for the camera’s internal shutter if one is present.
WARNING MicroMAX System User Manual Version 5.A Selecting the wrong shutter setting will result in improper functioning of the shutter and may cause premature shutter failure. texp Shutter tR Scan tc texp = Exposure Time tR = Readout Time tc = Shutter Compensation Time Shutter Type Compensation Time NONE 200 nsec Electronic 6.0 msec Remote (Prontor 23) 8.0 msec Small (Vincent 25 mm) 8.0 msec Large (Vincent 35 mm) 28.0 msec Figure 47.
Chapter 9 System Component Descriptions 97 Interface Card PCI Card: The standard interface card plugs-into the host computer's motherboard and provides the serial communication interface between the host computer and the ST-133A. Through WinView/32, the card can be used in either High Speed PCI or PCI(Timer) mode. High Speed PCI allows data transfer to be interruptdriven and can give higher performance in some situations. PCI(Timer) allows data transfer to be controlled by a polling timer.
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Chapter 10 Troubleshooting WARNING! Do not attach or remove any cables while the MicroMAX system is powered on. Introduction The following issues have corresponding troubleshooting sections in this chapter.
MicroMAX System User Manual Version 5.A Baseline Signal Suddenly Changes If you observe a sudden change in the baseline signal, you may have excessive humidity in the vacuum enclosure of the camera. Turn off the controller and have the camera repumped before resuming normal operation. Contact the factory Technical Support Dept. for information on how to refresh the vacuum. See page 150 for contact information.
Chapter 10 Troubleshooting 101 Controller Is Not Responding If this message pops up when you click on OK after selecting the "Interface Type" during Hardware Setup (under the WinView/32 Setup menu), the system has not been able to communicate with the Controller. Check to see if Controller has been turned ON and if the interface card, its driver, and the interface cable have been installed.
MicroMAX System User Manual Version 5.A Detector loses Temperature Lock The internal temperature of the camera is too high. This might occur if the operating environment is particularly warm or if you are trying to operate at a temperature colder than the specified limit. If this happens, an internal thermal overload switch will disable the cooler circuits to protect them. Typically, camera operation is restored in about ten minutes.
Chapter 10 Troubleshooting 103 Error occurs at Computer Powerup If an error occurs at boot up, either the interface card is not installed properly or there is an address or interrupt conflict. Turn off the computer, reinstall the interface card (make sure it is firmly seated), and reboot the system. If an error occurs while you are using the WinView/32 program, check the interface selection on the Hardware Setup|Interface tab page (WinView/32).
MicroMAX System User Manual Version 5.A As shown, there are no conflicts, allowing the three peripheral cards to operate properly. If the PCI Serial card were then installed, the BIOS would interrogate the PCI cards and may reassign them new address and interrupt values as follows.
Chapter 10 Troubleshooting 105 bus arbitration scheme assures that, as long as every PCI card conforms to the PCI guidelines, the on-board memory will never overflow. Unfortunately, there are some PCI peripheral cards that do not fully conform to the PCI guidelines and that take control of the bus for longer periods than the PCI specification allows. Certain video cards (particularly those that use the S3 video chip) are notorious in this respect.
MicroMAX System User Manual Version 5.A To Remove a Module: 1. Verify that the Controller has been turned OFF. 2. Rotate the two locking screws (one at the top of the module and one at the bottom) counterclockwise until they release from the chassis. 3. Then, grasp the module and pull it straight out. To Install a Module: Installing a module is a bit more complex because you first have to be sure the locking screws are aligned correctly. The following procedure is suggested. 1.
Appendix A Specifications CCD Arrays Spectral Range Typically: 370-900 for MicroMAX:512BFT and 1024B 400-1000 for MicroMAX:782Y, 782YHS 300-1080 for MicroMAX:1300Y, 1300YHS, and 1300YHS-DIF Types The following list is not necessarily current. Other chips may also be available. Contact the factory for up-to-date information.
MicroMAX System User Manual Version 5.A Temperature Control Setting Mechanism: Temperature is set by the application software. Display: The actual temperature can be displayed at the computer by the application software. Range: At 25° ambient, the MicroMAX camera will typically lock to: • -15°C with passive cooling and with the camera under vacuum. • -30°C with the accessory fan installed and under vacuum.
Appendix A Specifications 109 Inputs EXT SYNC: TTL input (BNC) to allow data acquisition to be synchronized with external events. Sense can be positive or negative going as set in software. Synchronization and Trigger Modes are discussed in Chapter 5. Outputs VIDEO: 1 V pk-pk from 75 Ω, BNC connector. Either RS-170 (EIA) or CCIR standard video as specified when system was ordered. Requires connection via 75 Ω cable that must be terminated into 75 Ω. : TTL output (BNC) for monitoring camera status.
MicroMAX System User Manual Version 5.A Memory (RAM): Minimum of 32 Mbytes; possibly more depending on experiment design and size of CCD Array. Operating System: Windows 95/ME/2000/XP or Windows NT for WinView/32. Windows 3.1 required for 16- bit versions of WinView. Interface: PCI High-Speed Serial I/O card is standard. Other types may be available. Contact factory for information. Note: Macintosh II support may be available. Contact factory for details. Miscellaneous Dimensions: See Appendix A.
Appendix B Outline Drawings 13.63 8.75 5. 25 Figure 51.
7.05 6.16 4.63 Version 5.A 2.11 C-MOUNT (1.00-32 THREAD) CCD at .690 OPTICAL DEPTH MicroMAX System User Manual 0.000 MOUNTING SURFACE 112 4.63 0.500 COOLING AIR INLET TYPICAL BOTH SIDES OPTIONAL TRIPOD MOUNT KIT (2550-0312) COOLING AIR OUTLET TYPICAL BOTH SIDES GAIN SWITCH ACCESS DB-25 MALE TO CONTROLLER 2.41 2.25 3.16 0.94 ALLOW 1.5” FOR ELECTRICAL CONNECTION 1.14 1.54 Figure 52.
Outline Drawings 8.18 4.63 113 CCD at 1.831 OPTICAL DEPTH F-MOUNT (2 3/8” - 20 THREAD NIKON ADAPTER SHOWN 0.000 Appendix B 4.63 NIKON F-MOUNT 0.50 COOLING AIR INLET TYPICAL BOTH SIDES OPTIONAL TRIPOD MOUNT KIT (2550-0312) COOLING AIR OUTLET TYPICAL BOTH SIDES GAIN SWITCH ACCESS DB-25 MALE TO CONTROLLER 3.16 0.94 1.14 1.54 Figure 53.
4.63 2.25 1.38 4.63 (3X) #10-32 UNC-2B X .17 INCHES DEEP EQUALLY SPACED ON A Ø3.880 BOLT CIRCLE AT 120° APART 2.58 0.500 ALLOW 1.5” FOR ELECTRICAL CONNECTION 1.20 MOUNTING 0.000 SURFACE 2.600 0.063 2.26 0.886 CCD COOLING AIR OUTLET TYPICAL BOTH SIDES 6.50 NOTES: 1. WEIGHT: 7.0 LBS. 2. POWER DISSIPATION: 90 WATTS AT FULL POWER. 3. AIRFLOW: 30 CFM FAN CAPACITY AT FULL POWER. OPTIONAL TRIPOD MOUNT KIT (2550-0312) 7.21 COOLING AIR INLET TYPICAL BOTH SIDES 3.16 0.94 1.54 1.
4.63 2.25 1.38 4.63 (3X) #10-32 UNC-2B X .17 INCHES DEEP EQUALLY SPACED ON A Ø3.880 BOLT CIRCLE AT 120° APART 2.48 0.500 ALLOW 1.5” FOR ELECTRICAL CONNECTION 1.20 MOUNTING 0.000 SURFACE 2.600 0.063 2.16 0.786 CCD COOLING AIR OUTLET TYPICAL BOTH SIDES 6.40 NOTES: 1. WEIGHT: 7.0 LBS. 2. POWER DISSIPATION: 90 WATTS AT FULL POWER. 3. AIRFLOW: 30 CFM FAN CAPACITY AT FULL POWER. OPTIONAL TRIPOD MOUNT KIT (2550-0312) 7.11 COOLING AIR INLET TYPICAL BOTH SIDES 3.16 0.94 1.54 1.
SECTION A A HOUSING 2.49 2.19 “C” MOUNT ADAPTER W/ SHUTTER CCD 0.00 QUARTZ WINDOW INNER NOSE 2.49 A 8 HOLES, TAPPED 4–40 X 0.25 DP, EQ. SP. @ 45° ON 4.100 B.C. A 45 0 .0 1.41 0.00 “C” MOUNT TAPPED 1.00–32 UNEF 2B THRU 0.00 0.00 VACUUM PORT 2.19 0.00 0.75 25 PIN CONNECTOR 0.75 Note: All dimensions are in inches 1.53 0.90 0.30 0.00 1/4–20 X 3/8 MTG. (4 PLACES) 116 MicroMAX System User Manual Version 5.A Figure 56.
SECTION A A HOUSING 2.49 2.19 “C” MOUNT ADAPTER CCD 0.00 QUARTZ WINDOW INNER NOSE 2.49 A 8 HOLES, TAPPED 4–40 X 0.25 DP, EQ. SP. @ 45° ON 4.100 B.C. A 45 0 .0 Figure 57. 5 MHz Round Head Camera: C-Mount Adapter 1.41 0.00 “C” MOUNT TAPPED 1.00–32 UNEF 2B THRU 0.00 0.00 0.00 0.75 40 PIN CONNECTOR 0.75 15 PIN CONNECTOR Note: All dimensions are in inches 1.53 0.66 0.14 0.00 1.58 AIR FLOW 1/4–20 X 3/8 MTG, (4 PLACES) 3.12 3.30 VACUUM PORT 2.19 Appendix B Outline Drawings 117 1.
0.90 1.83 (FOCAL PLANE) SECTION A A HOUSING 2.49 CCD 0.00 QUARTZ WINDOW 2.19 INNER NOSE 2.49 A 52 0 .0 45 .50 8 HOLES, TAPPED 4–40 X 0.25 DP, EQ. SP. @ 45° ON 4.100 B.C. A Figure 58. 1 MHz Round Head Camera: F-Mount Adapter 2.18 0.00 “F” MOUNT ADAPTER (2 3/8"-20 THREAD NIKON ADAPTER SHOWN) 0.00 75.00 0.00 VACUUM PORT 2.19 0.75 25 PIN CONNECTOR 0.00 3.89 4.07 Note: All dimensions are in inches 1.53 0.90 0.30 0.00 1/4–20 X 3/8 MTG.
Appendix C Kinetics Mode Introduction Kinetics mode uses the CCD to expose and store a limited number of images in rapid succession. The time it takes to shift each line (or row) on the CCD is as short as a few hundred nanoseconds to few microseconds, depending on the CCD. Therefore the time between images can be as short as a few microseconds. Kinetics mode allows frame transfer CCDs to take time-resolved images/spectra.
MicroMAX System User Manual Version 5.A Kinetic Timing Modes Kinetics mode operates with three timing modes: Free Run, Single Trigger, and Multiple Trigger. Figure 60. Hardware Setup dialog box Figure 61. Experiment Setup dialog box Free Run In the Free Run Kinetics mode, the controller takes a series of images, each with the Exposure time set through the software (in WinView32, the exposure time is set on the Experiment Setup|Main tab page).
Appendix C Kinetics Mode START ACQUIRE 121 START ACQUIRE command from the software issent automatically when ACQUIRE or FOCUS is clicked on in the software. Exposure Shutter Monitor Signal Shift Not SCAN Signal Shutter opening time Shutter closing time Readout Figure 62. Free Run Timing Diagram Single Trigger Single Trigger Kinetics mode takes an entire series of images with each External Trigger Pulse (applied at the Ext. Sync BNC on the control board of ST133).
MicroMAX System User Manual START ACQUIRE Version 5.A START ACQUIRE command from the software issent automatically when ACQUIRE or FOCUS is clicked on in the software. External Triggers Exposure Shutter Monitor Signal Shift Not SCAN Signal Shutter opening time Figure 64.
Appendix D Virtual Chip Mode Introduction Virtual Chip mode (a WinView/32 option) is a special fast-acquisition technique that allows frame rates in excess of 100 fps to be obtained. For the Virtual Chip selection to be present, it is necessary that: • the system be a 1 MHz MicroMAX, • that the camera have a frame transfer chip (MicroMAX:512BFT) and, • that the file Wxvchip.opt be present in the same directory as the executable WinView/32 program.
MicroMAX System User Manual Version 5.A The table below shows the minimum exposure time per frame (msec/Frame) and the number of frames per second (FPS) for several ROIs. Note that these numbers are provided for the 1 MHz and the 100 kHz readout rates. 1 MHz ROI 100 kHz (msec/Frame) (FPS) (msec/Frame) (FPS) 164 x 164 29.8 33.6 287 3.5 96 x 96 10.9 91.7 102 9.8 84 x 84 8.65 115 79.8 12.5 64 x 64 5.26 190 47.6 21.0 56 x 56 4.22 237 37.4 36.7 47 x 47 3.08 324 26.9 37.
Appendix D Virtual Chip Mode 125 Assumptions: • You are familiar with the WinView/32 software and have read the hardware manuals. • Masking is for a 47x47 pixel Virtual Chip with its origin at 1,1. System Connection Diagram: Detector-Controller TAXI cable (Serial Com) 110/220 Camera Detector Serial 110/220 Controller Microscope Computer EXPERIMENT Figure 66. System Diagram Procedure: 1. Verify that the power is OFF for ALL system components (including the host computer). 2.
MicroMAX System User Manual • Shutter Type: None • LOGIC OUT Output: Shutter • Readout Mode: Frame Transfer Version 5.A Interface tab card • Type: the appropriate interface card. For this procedure, the selection is High Speed PCI. Cleans/Skips tab card • Number of Cleans: 1 • Number of Strips per Clean: 512 • Minimum Block Size: 2 • Number of Blocks: 5 9. From the Acquisition menu, select Experiment Setup and enter the following settings: Main tab card • Exposure Time: Enter a value.
Appendix D Virtual Chip Mode 127 11. Click on the Load Default Values button. This enters the default ROI values. These values are: Start pixels of 1,1; End pixels based on the Chip Y and Chip X dimensions; and Groups of 1. • Region of Interest: The settings below assume a 47x47 pixel ROI (i.e., the entire virtual chip). An ROI that is a subset of the virtual chip can be defined.
MicroMAX System User Manual Version 5.A Experimental Timing Triggering can be achieved through the software via the Software Trigger timing mode (selectable on the Experiment Setup dialog box, Timing Mode tab page) or it can be achieved via the Ext Sync input on the rear of the camera. Triggering from the Ext Sync input allows you to acquire a single image per TTL pulse.
Appendix D Virtual Chip Mode 129 Load Default Values: Fills in the region of interest X and Y End values based on the Chip X and Y Dimension entries. By default, the ROI origin is at 1,1 and the Group values are both 1. Download Virtual Chip Definition: Sends the virtual chip parameter values to the controller’s non-volatile memory. If a virtual chip definition is already stored there, you will be given an overwrite warning.
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Appendix E Repumping the Vacuum Introduction Round head cameras are normally shipped with a vacuum level of ~10 mTorr or better to assure proper cooling performance and to prevent condensation from collecting on the CCD. This condensation obscures or interferes with optical signals, and can leave behind harmful contaminants. In time, the vacuum level could deteriorate to where achieving temperature lock will no longer be possible.
MicroMAX System User Manual Version 5.A Figure 68. Vacuum Connector Required for Pumping • Phillips screwdriver and a 3/16" nut driver, required to remove the back plate from the camera. Vacuum Pumpdown Procedure The instructions that follow are for a 1 MHz or 100kHz/1 MHz round head camera only. Contact the factory if you have a 5 MHz round head camera that you think may need to be repumped 1. Remove the back cover of the camera (see Figure 69).
Appendix E Repumping the Vacuum 133 7. Remove the vacuum system from the Vacuum Connector. While turning the top knob counterclockwise, remove the Vacuum Connector from the camera. Replace the back cover. Figure 70. Attaching the Vacuum Connector Figure 71.
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Appendix F Spectrometer Adapters Roper Scientific offers a variety of spectrometer adapters for rectangular head (NTE) MicroMAX systems. The mounting instructions for these adapters are organized by spectrometer model, camera type, and adapter kit number. The table below crossreferences these items with the page number for the appropriate instruction set. Spectrometer Camera Type Adapter Kit No.
MicroMAX System User Manual Version 5.A Chromex 250 IS (NTE with or without shutter) 4 2 3 1 5 Qty P/N Description 1. 1 2517-0901 Plate, Adapter-Female 2. 4 2826-0283 Screw, 10-32 × 3/4, Socket head, Stainless Steel, Hex, Black 3. 1 2518-0107 Adapter-Male, HR320 4. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel 5. 1 2826-0082 Set Screw, 10-32 × 1/4, Stainless Steel, Allen Hex, Nylon Tip Assembly Instructions 1.
Appendix F Spectrometer Adapters 137 ISA HR 320 (NTE with or without shutter) Remove spectrometer cover for these screws. 1 4 3 2 5 5 Qty P/N Description 1. 1 2518-0106 Adapter-Female, HR320 2. 3 2826-0087 Screw, M5-10, Flat Head, Socket, Stainless Steel 3. 1 2518-0107 Adapter-Male, HR320 4. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel 5. 2 2826-0082 Set Screw, 10-32 × 1/4, Stainless Steel, Allen Hex, Nylon Tip Assembly Instructions 1.
MicroMAX System User Manual Version 5.A ISA HR 640 (NTE with or without shutter) 5 4 1 3 2 Qty P/N Description 1. 1 2518-0203 Adapter-Female, HR640 2. 4 2826-0144 Screw, M4-.7 × 14, Socket Head Cap, Stainless Steel 3. 1 2518-0107 Adapter-Male, HR320 4. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel 5. 2 2826-0082 Set Screw, 10-32 × 1/4, Stainless Steel, Allen Hex, Nylon Tip Assembly Instructions 1.
Appendix F Spectrometer Adapters 139 JY TRIAX family (NTE without shutter) Flanged Spectrometer Mount Remove 4 screws 1 2 Qty P/N Description 1. 1 2518-1000 Adapter, TRIAX, NTE, 7377, 7376, 7413 2. 4 2826-0191 Screw, 10-32 × 5/8, Socket Head, Stainless Steel, Hex, Black Typically, the adapter is shipped already mounted to the camera. The following procedure is provided in case you have ordered a JY TRIAX adapter for a shutterless MicroMAX rectangular head (NTE) camera that you already own.
MicroMAX System User Manual Version 5.A SPEX 270M (NTE with or without shutter) 4 1 3 2 5 Qty P/N Description 1. 1 2518-0691 Female Adapter Plate, 2.400 ID 2. 6 2826-0068 Screw, 6-32 × 3/8, Socket Head, Cap, Stainless Steel 3. 1 2518-0690 Adapter, Focusing, Male, Spec 270 4. 3 2826-0127 Screw, 10-32 × 1/4, Button Head Allen Hex, Stainless Steel 5. 2 2826-0073 Screw 6-32 × 1/8, Set, Allen Hex, Brass Tip Assembly Instructions 1. Remove the cover of the spectrometer. 2.
Appendix F Spectrometer Adapters 141 SPEX 500M (NTE with or without shutter) 1 2 4 3 5 Qty P/N Description 1. 1 2517-0214 Adapter-Female, Spex 500m 2. 8 2826-0170 Screw, 1/4-20 × 0.51, Low Socket Head Cap, Black 3. 1 2518-0223 Adapter-Male, Spex 500m 4. 3 2826-0134 Screw, 10-32 × 1/4, Flat Head Slot, Stainless Steel 5. 2 2826-0055 Screw, 8-32 × 14, Set Allen Hex, Nylon Assembly Instructions 1.
MicroMAX System User Manual Version 5.A SPEX TripleMate (NTE with or without shutter) 3 1 2 7 6 5 4 Qty P/N Description 1. 1 2518-0184 Adapter-Male, LN/TE, CCD/For Spex TripleMate 2. 4 2826-0128 Screw, 10-32 × 5/8, Socket Head Cap, Stainless Steel, 3. 1 2517-0163 Slit Mount, Spex 4. 4 2826-0129 Screw, 1/4-20 × 3/4, Socket Head Cap, Stainless Steel 5. 3 2826-0127 Screw, 10-32 × 1/4, Button Head, Hex, Stainless Steel 6. 1 2518-0185 Adapter-Female, Flange Spex 7.
Declarations of Conformity This section of the MicroMAX manual contains the declarations of conformity for MicroMAX systems. MicroMAX systems encompass RTE (round thermoelectrically-cooled) and NTE (rectangular thermoelectrically-cooled) camera heads and their associated controllers.
DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibility, that the product MicroMAX SYSTEM With RTE/CCD CAMERA, To which this declaration relates, is in conformity with general safety requirement for electrical equipment standards: IEC 1010-1:1990, EN 61010-1:1993/A2:1995 EN 50082-1:1992, (EN 61000-4-2, EN 61000-4-3, EN 61000-4-4, 1995) EN 55011 for GROUP 1, CLASS A, 1991, EN 61000-3-2, 1994 Which follow the pro
DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibility, that the product ST-133A 5MHz CONTROLLER With RTE CAMERA HEAD, To which this declaration relates, is in conformity with general safety requirement for electrical equipment standards: IEC 1010-1:1990, EN 61010-1:1993/A2:1995 EN 55011 for Group 1, Class A, 1991, EN50082-1, 1991 (EN 61000-4-2, EN 61000-4-3, EN 61000-4-4) Which follow the provisions of the C
DECLARATION OF CONFORMITY We, , ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619, Declare under our sole responsibility that the product ST-133A 1 MHz HIGH POWER CONTROLLER w/NTE CAMERA HEAD, To which this declaration relates, is in conformity with general safety requirement for electrical equipment standards: IEC 1010-1:1990, EN 61010-1:1993/A2:1995 EN 55011 for Group 1, Class A, 1991, EN 50082-1, 1991 (EN 61000-4-2, EN 61000-4-3, EN 61000-4-4), Which follow the provisi
Warranty & Service Limited Warranty: Roper Scientific Analytical Instrumentation Roper Scientific, Inc. (“Roper Scientific,” us,” “we,” “our”) makes the following limited warranties. These limited warranties extend to the original purchaser (“You”, “you”) only and no other purchaser or transferee. We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary.
MicroMAX System User Manual Version 5.A Sealed Chamber Integrity Limited 24 Month Warranty Roper Scientific warrants the sealed chamber integrity of all our products for a period of twenty-four (24) months after shipment. If, at anytime within twenty-four (24) months from the date of delivery, the detector should experience a sealed chamber failure, all parts and labor needed to restore the chamber seal will be covered by us.
Warranty & Service 149 Owner's Manual and Troubleshooting You should read the owner’s manual thoroughly before operating this product. In the unlikely event that you should encounter difficulty operating this product, the owner’s manual should be consulted before contacting the Roper Scientific technical support staff or authorized service representative for assistance.
MicroMAX System User Manual Version 5.A any warranties implied by state law or non-U.S. laws, as hereby limited, the forgoing warranty is exclusive and in lieu of all other warranties, guarantees, agreements, and similar obligations of manufacturer or seller with respect to the repair or replacement of any parts. In no event shall Roper Scientific’s liability exceed the cost of the repair or replacement of the defective product or part. 11.
Index #-B 64-pin DIN connector 92, 106 A/D converters dual 72 specifications 109 AC power requirements 22 Accessories, alignment of 45 Actual exposure time 53, 56 Adapter instructions Chromex 250 IS 136 ISA HR 320 137 ISA HR 640 138 JY TRIAX 139 SPEX 270M 140 SPEX 500M 141 SPEX TripleMate 142 Air-circulation requirement 91 Analog channels 72 Analog/Control module 92, 93 Applications 13 Back-filled 131 Background DC level 62 Background subtraction 50 Back-plane 92 Baseline signal 34, 62 ST-133A zero adjustme
MicroMAX System User Manual Connectors (cont.) ST-133A, Serial COM ST-133A, TTL In/Out ST-133A, Video Output Contact information Continuous Cleans.
Index 153 Free Run (cont.
MicroMAX System User Manual Version 5.
Index 155 ST-133A Controller (cont.
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USB 2.0 Interface Addendum Introduction Either a TAXI® or a USB 2.0 protocol can be used to interface a host computer with an ST-133A Controller that supports digitization rates up to and including 1 MHz. The Interface card installed in the ST-133A determines the protocol used. The advantages to the USB 2.0 interface are that it uses a much higher data transfer rate than many common serial data formats (such as the TAXI protocol) and it simplifies the connection to external devices.
USB 2.0 Interface Addendum USB 2.0 Limitations • Maximum cable length is 5 meters (16.4 feet) • 1 MHz is currently the upper digitization rate limit for the ST-133A Controller. • Large data sets and/or long acquisition times may be subject to data overrun because of host computer interrupts during data acquisition. • The Roper Scientific PC Interface Library (EasyDLLS) does not support USB 2.0. • Some WinX (WinView and WinSpec) 2.5.X features are not fully supported with USB 2.0.
USB 2.0 Interface Addendum Installing the Application Software Installation is performed via the WinView/32 or WinSpec/32 installation process. If you are installing WinView or WinSpec for the first time and have a TAXI interface, you should run the installation before the PCI interface card is installed in the host computer. On the Select Components dialog box (see Figure 1), click on the AUTO PCI button to install the interface card drivers (PCI and the Roper Scientific USB drivers) and the Figure 1.
USB 2.0 Interface Addendum To Update the OrangeUSB USB 2.0 Driver: This procedure is highly recommended when a laptop computer will be used to communicate with the ST-133A. As stated before, we recommend the SIIG, Inc. USB 2.0 PC Card, Model US2246 if USB 2.0 is not native to the laptop's motherboard. To reduce the instances of data overruns and serial violations, the OrangeUSB USB 2.
USB 2.0 Interface Addendum To Install the Roper Scientific USB2 Interface: The following information assumes that: • You have verified that the host computer meets the required specifications for USB 2.0 communication with the camera system (see page 1). • A USB 2.0 board and its driver are installed in the host computer. • The ST-133A has an installed USB 2.0 Interface Control module. • You have already installed the WinView/32 or WinSpec/32 software (versions 2.5.15 and higher). Versions 2.5.
USB 2.0 Interface Addendum Figure 2. RSConfig dialog box 4. Open WinView or WinSpec and, from Setup|Hardware…, run the Hardware Wizard. 5. When the PVCAM dialog box (Figure 6) is displayed, click in the Yes radio button, click on Next and continue through the Wizard. After the Wizard is finished, the Controller/Camera tab card will be displayed with the Use PVCAM checkbox selected. You should now be able to set up experiments and acquire data. Figure 3.
USB 2.0 Interface Addendum USB 2.0 and System On/Off Sequences The following on/off sequences are specific to the USB 2.0 interface: 1. The ST-133A must be turned on before WinView/32 or WinSpec/32 is opened to ensure communication between the controller and the computer. If WinView or WinSpec is opened and the ST-133A is off, many of the functions will be disabled and you will only be able to retrieve and examine previously acquired and stored data.
USB 2.0 Interface Addendum TAXI interface module and Roper Scientific PCI card. If this is not the case and data overruns continue to occur, contact Technical Support (see page 13 for contact information). Demo is only Choice on Hardware Wizard:Interface dialog If RSConfig.exe has not been run and there is not an installed Roper Scientific high speed PCI card, the Hardware Wizard will only present the choice "Demo" in the Interface dialog box (Figure 4).
USB 2.0 Interface Addendum 5. You should now be able to open WinView or WinSpec and, from Setup|Hardware…, run the Hardware Wizard. 6. When the PVCAM dialog box (Figure 6) is displayed, click in the Yes radio button, click on Next and continue through the Wizard. After the Wizard is finished, the Controller/Camera tab card will be displayed with the Use PVCAM checkbox selected. You should now be able to set up experiments and acquire data. Figure 6.
USB 2.0 Interface Addendum 4. When the RSConfig dialog box (Figure 5) appears, you can change the camera name to one that is more specific or you can keep the default name "Camera2". When you have finished, click on the Done button. Figure 8. RSConfig dialog box: Two Camera Styles 5. Using Notepad or a similar text editor, open PVCAM.INI, which is located in the Windows directory (C:\WINNT, for example).
USB 2.0 Interface Addendum Figure 9. Hardware Wizard: PVCAM dialog box Detector Temperature, Acquire, and Focus are Grayed Out These functions and others will be deactivated if you have installed a camera being run under USB 2.0 and have opened WinView/32 or WinSpec/32 without having first turned on the ST-133A. They will also be deactivated if you have installed a camera being run under USB 2.0 and a Princeton Instruments high speed PCI card was also detected when RSConfig.exe was run. 1.
USB 2.0 Interface Addendum 5. Using Notepad or a similar text editor, open PVCAM.INI, which is located in the Windows directory (C:\WINNT, for example). If the contents of the file look like: Change the headings so the contents now look like: [Camera_1] Type=1 Name=Camera1 Driver=rspipci.sys Port=0 [Camera_2] Type=1 Name=Camera1 Driver=rspipci.sys Port=0 [Camera_2] Type=1 Name=Camera2 Driver=apausb.sys Port=0 [Camera_1] Type=1 Name=Camera2 Driver=apausb.
USB 2.0 Interface Addendum No CCD Named in the Hardware Wizard:CCD dialog Figure 12. Hardware Wizard: Detector/Camera/CCD dialog box If you have installed a USB 2.0 Interface Module in your ST-133A, a blank field may be displayed in the Detector/Camera/CCD dialog box (Figure 12) if the ST-133 controller was made before January 2001. Earlier versions of the ST-133 did not contain nonvolatile RAM (NVRAM), which is programmed with information about the controller and the camera.
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