4411-0070 Version 2.
Copyright 2001 Roper Scientific, Inc. 3660 Quakerbridge Rd Trenton, NJ 08619 TEL: 609-587-9797 FAX: 609-587-1970 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Roper Scientific, Inc. Printed in the United States of America. SpectroPro is a registered trademark of Acton Research Corporation. TAXI is a registered trademark of AMD Corporation The information in this publication is believed to be accurate as of the publication release date.
Table of Contents Introduction.......................................................................................................... 7 Manual Overview................................................................................................................7 Safety Related Symbols Used In This Manual ...................................................................7 Chapter 1 General Information ........................................................................... 9 Description ...........
iv NTE/CCD Detector Manual Version 2.A Chapter 5 Microscopy Applications................................................................. 27 Introduction.......................................................................................................................27 Mounting the Detector on the Microscope .......................................................................28 C-Mount ................................................................................................................
Table of Contents v Figures Figure 1. NTE/CCD Detectors and Cameras ....................................................................9 Figure 2. NTE Detector Rear Panel .................................................................................11 Figure 3. Annotated Spectrograph Drawing ....................................................................12 Figure 4. Adapter for a Deep Focal Plane Spectrograph......................................................13 Figure 5.
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Introduction Manual Overview This manual provides the user with all the information needed to install a NTE/CCD Detector and place it in operation. Topics covered include a detailed description of the NTE Detector, installation, microscopy applications, cleaning, specifications and more. Chapter 1, General Information provides an overview of the NTE/CCD Detector.
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Chapter 1 General Information Figure 1. NTE/CCD Detectors and Cameras Description The NTE/CCD air-cooled detector or camera is ideally suited for medium light level applications. State-of-the-art CCD arrays are available for the NTE/CCD that enable outstanding performance in a wide range of applications for spectroscopy, biological imaging, and physical science investigations. Design NTE/CCD detectors have three distinct sections. The front vacuum enclosure contains the CCD array seated on a cold finger.
NTE/CCD Detector Manual Version 2.A The NTE/CCD detector is available in both C-mount and F-mount configurations. See Chapter 5 for detailed information on microscopy.
Chapter 2 Detector Setup This chapter covers the setup procedures for both imaging and spectroscopic applications. General Instructions The following items are applicable to both imaging and spectroscopic systems. Connecting the Detector Each detector is supplied with a cable to connect to the controller. Make sure that the controller is off, and then connect the larger end of the cable to the port marked Detector on the controller. The detector end of the cable is secured by a slide-lock latch.
NTE/CCD Detector Manual Version 2.A suitable Gain setting. In situations where the A/D range exceeds that of the array, it will generally be better to set the Gain to HI so that the signal can be spread over as much of the A/D range as possible. This is a particularly important consideration in absorbance measurements. Users who consistently measure low-level signals may wish to select HI, which reduces some sources of noise.
Chapter 2 Detector Setup 13 Square-format CCDs can also be used for spectroscopy, although they are more often used in imaging applications. For square format CCDs (e.g. 512 × 512 or 1300 × 1340), the user may orient the CCD to achieve binning along either direction of the CCD. Binning along the rows (perpendicular mode) minimizes cross-talk and is therefore better for multi-spectral applications. The drawback to this method is that scanning is slower and noise may increase somewhat.
NTE/CCD Detector Manual Version 2.A Mounting Directions 1. Mount the flange to the detector using the two half-rings and the screws provided. Note that the tapered side of each half-ring faces the adapter (Figure 5). Flange 2. Next, thread the 10-32 hex screws halfway into three of the six tapped holes in the spectrograph’s exit plane. 3. Position the detector so the three hex head screws line up with the openings in the adapter flange. 4.
Chapter 2 Detector Setup Second Shutter Type Mounting Directions The second shutter mount, used with all Acton Research spectrographs, requires no disassembly. Mount it to the spectrograph as shown in Figure 7. 15 Spectrometer Adapter Figure 7. Entrance Slit Shutter for Acton Spectrographs The shutter cable should be connected to the Shutter Power connector on the rear panel of the NTE/CCD Detector or to the Shutter Power connector on the ST-133 Controller.
NTE/CCD Detector Manual Version 2.A To Mount the Lens on the Detector: Set screws to lock front part of adapter in place 1. Locate the large indicator dot on the side of the lens. 2. Note the corresponding dot on the front side of the adapter. 3. Line up the dots and slide the lens into the adapter. 4. Turn the lens counterclockwise until a click is audible. The lens is now locked in place. 5. In addition to the focusing ring of the lens, there is provision for focusing the adapter itself.
Chapter 3 Cooling the Detector Introduction The NTE/CCD detector is designed for cooled operation. A four-stage Peltier effect thermoelectric cooler, driven by closed-loop proportional-control circuitry, cools the CCD. A thermal sensing diode attached to the cooling block of the detector monitors its temperature. An internal fan draws air through the heat-exchanger to remove the waste heat and the warm air is exhausted through openings in the side of the housing.
NTE/CCD Detector Manual Version 2.A 4. On the top side of the Temp knob is a rectangular window that denotes hundreds of degrees C. Each complete turn of the knob is -100°C. Around the moveable part of the knob are numbers from 0 to 99, in increments of 2. Turn the knob until the correct value (0) appears in the hundreds’ box. 5. Then turn the knob until the desired value between 0 and 70 appears below the box. 6. Turn the locking tab clockwise to lock the Temp knob in place.
Chapter 3 Cooling the Detector 19 Detector loses Temperature Lock The internal temperature of the detector is too high. This might occur if the operating environment is particularly warm or if you are attempting to operate at a temperature colder than the specified limit. If this happens, an internal thermal overload switch will disable the cooler circuits to protect them. Typically, detector operation is restored in about ten minutes.
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Chapter 4 Focusing Introduction Detectors for either imaging or spectroscopic applications must be focused for maximum resolution. Imaging applications require adjustment of both the lens and the lens adapter. Spectroscopic applications demand both focusing and alignment of the detector to the spectrograph. One of the limitations of scientific non-video rate detectors has been their difficulty in focusing and locating fields of view.
NTE/CCD Detector Manual Version 2.A fluorescent overhead lamps have good calibration lines as well. If there are no “line” sources available, it is possible to use a broad band source, such as tungsten, for the alignment. If this is the case, use a wavelength setting of 0.0nm for alignment purposes. 2. With the Model SP300i properly connected to the controller, turn the power on and wait for the spectrograph to initialize. Then set it to 435.8 nm if using a mercury lamp or to 0.
Chapter 4 Focusing 23 Throughput: The throughput of a lens is determined by its aperture, which can ordinarily be set to a number of different values, or f/ stops. The higher the number the smaller the aperture and the lower the throughput. Depth of field considerations make it imperative that focusing be done at maximum aperture (smallest f/). Resolution: This is a measure of the sharpness of the lens, that is, of its ability to resolve two closely spaced lines.
NTE/CCD Detector Manual Version 2.A Imaging Field of View When used for two-dimensional imaging applications, PI CCD detectors closely imitate a standard 35 mm detector. Since the CCD is not the same size as the film plane of a 35 mm detector, the field of view at a given distance is somewhat different. CCD Object Lens Princeton Instruments, Inc S O B D Figure 10. Imaging Field of View D = distance between the object and the CCD (the CCD is 46.
Chapter 4 Focusing 25 Remount the lens to the adapter and set the lens focus adjustment to the target distance. 4. Power up the system and, using the application software, select FreeRun, and Safe mode (Asynchronous). 5. Choose a fast exposure (0.1 s) and begin data collection by selecting Focus (WinView/32). 6. Note the image on the computer monitor. If it is washed out because the CCD is saturated, reduce the exposure time. If it is too dark, increase the exposure time. 7.
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Chapter 5 Microscopy Applications Introduction This chapter discusses the setup and optimization of your digital imaging system as applied to microscopy. Since scientific grade cooled CCD imaging systems are usually employed for low light level microscopy, the major goal is to maximize the light throughput to the detector. In order to do this, the highest Numerical Aperture (NA) objectives of the desired magnification should be used.
NTE/CCD Detector Manual Version 2.A Mounting the Detector on the Microscope The detector is connected to the microscope via a standard type mount coupled to a microscope specific adapter piece. There are two basic detector-mounting designs, the C-mount (standard) and the F-mount (optional). The C-mount employs a standard size thread to connect to the detector to the adapter while the F-mount uses a tongue and groove type mechanism to make the connection.
Chapter 5 Microscopy Applications 29 Diagnostic Instruments Bottom Clamp Type Microscope Type Leica DMR L-clamp Leitz All types NLW-clamp Nikon Optiphot, Diaphot, Eclipse O-clamp Olympus BH-2, B-MAX, IMT-2 V-clamp Zeiss Axioscope, Axioplan, Axioplan 2, Axiophot Z-clamp Zeiss Axiovert ZN-clamp Table 1. Bottom Clamps for Different Microscopes To assemble the pieces, first pick up the detector and look for the black dot on the front surface.
NTE/CCD Detector Manual Version 2.A 1X HRP 100-NIK L ZN O NLW Z V Figure 11. Diagnostic Instruments Bottom Clamps for Different Microscopes 1X HRP 100-NIK "L" bottom clamp Figure 12.
Chapter 5 Microscopy Applications 31 Note: The detector lens mount itself also has a focus adjustment. Although it is unlikely that you would ever need to use this adjustment in operation with a microscope (it is preferable that you use the relay-lens focus adjustment), it could be used if necessary. The procedure for using the adjustment is provided in Chapter 4. CAUTION Microscope optics have very high transmission efficiencies in the infrared region of the spectrum.
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Chapter 6 Operation Introduction Once the NTE/CCD Detector has been installed and its optics adjusted as explained in the preceding chapters, operation of the detector is basically straightforward. In most applications you simply establish optimum performance using the Focus mode (WinView/32 or WinSpec/32) and then do actual data acquisition in the Acquire mode. There are many of possible software considerations that are addressed in the software manual.
CAUTION NTE/CCD Detector Manual Version 2.A If you observe a sudden change in the baseline signal you may have excessive humidity in the vacuum enclosure of the detector. Turn off the system and have the detector repumped before resuming normal operation. Contact the factory Technical Support Dept. for information on how to refresh the vacuum. See page 50 for contact information.
Chapter 6 Operation Note that a camera having the 35 mm shutter can only be used with an ST-133 equipped with the 70 V shutter option. An ST-133 that has the 70 V shutter drive option can be identified by the 70 V OPT label on its rear panel as shown in Figure 14. Note that there is a Shutter Setting push switch that sets the shutter drive voltage. Each shutter type, whether internal or external, requires a different setting. Consult the table below to determine the proper setting for your shutter.
NTE/CCD Detector Manual Version 2.A Shutter Overheating The 25 mm remote-mounted shutter for spectroscopy has a built-in thermal interlock to prevent overloading of its coil. If run at a high repetition rate, the shutter may heat enough to trigger the interlock, disabling the shutter. If your shutter suddenly stops running, stop the experiment and wait. The shutter should resume functioning when it has cooled down sufficiently, typically within an hour.
Chapter 7 Cleaning WARNING Turn off all power to the equipment and secure all covers before cleaning the units. Otherwise, damage to the equipment or injury to you could occur. Controller and Camera Although there is no periodic maintenance that must be performed on the NTE/CDD Detector, users are advised to wipe it down with a clean damp cloth from time to time. This operation should only be done on the external surfaces and with all covers secured. In dampening the cloth, use clean water only.
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Appendix A Specifications Controller Requirement Requires ST-133 or ST-138 with high power modification. Standard ST-133 or ST-138 Controllers cannot meet the NTE’s power requirements. CCD Arrays Note: The following list may not be current. Contact the factory for up-to-date information on available chips and chip performance specifications.
NTE/CCD Detector Manual Version 2.A Power Maximum internal heat dissipation in watts: 90 Cooling The internal fan circulates cooling air over the heat exchanger. Openings in the side of the housing provide the necessary circulation access. Fan capacity at full power is 30 cfm. Attainable lock temperature will vary depending on array size. Cooling performance is improved with fresh vacuum. Contact the factory Technical Support Dept. for information on refreshing the vacuum.
Appendix B Outline Drawings 6.92 .593 CCD .000 FLANGE Note: Dimensions are in inches. 2.598±.001 CENTERING BOSS .279 WINDOW COOLING AIR INLET TYPICAL BOTH SIDES COOLING AIR OUTLET TYPICAL BOTH SIDES Figure 15. Spectrometer Mount: Side View Æ.358 HOLE TO Æ.203 SLOTS FOR #10-32 BOLT MOUNTING CIRCLE (3 PLACES) Æ3.600 4.63 GAIN SWITCH ACCESS (NOT ACTIVE) DB-25 MALE TO CONTROLLER 4.63 3.16 .94 1.14 1.54 Figure 16.
NTE/CCD Detector Manual Figure 17. F-Mount: Side View F-MOUNT (2 3/8 - 20 THREAD NIKON ADAPTER SHOWN 4.63 4.63 Figure 18. F-Mount: Front and Back View Version 2.
7.05 6.16 2.11 0.720 CCD Outline Drawings 0.000 MOUNTING SURFACE Appendix B 2.41 2.25 0.500 COOLING AIR INLET TYPICAL BOTH SIDES OPTIONAL TRIPOD MOUNT KIT (2550-0312) COOLING AIR OUTLET TYPICAL BOTH SIDES ALLOW 1.5” FOR ELECTRICAL CONNECTION Figure 19. C- Mount: Side View C-MOUNT (1.00-32 THREAD) 4.63 4.13 Figure 20.
NTE/CCD Detector Manual Version 2.A 1.52 2.97 2.25 ALLOW 1.5” FOR ELECTRICAL CONNECTION Figure 21. Fiber Optic Coupled: Side and Bottom Views Figure 22.
DECLARATION OF CONFORMITY We, ROPER SCIENTIFIC (PRINCETON INSTRUMENTS) 3660 QUAKERBRIDGE ROAD TRENTON, NJ 08619 Declare under our sole responsibility, that the product ST-133A 1MHz HIGH POWER CONTROLLER w/NTE CAMERA HEAD, To which this declaration relates, is in conformity with general safety requirement for electrical equipment standards: IEC 1010-1:1990, EN 61010-1:1993/A2:1995 EN 55011 for Group 1, Class A, 1991, EN50082-1, 1991 (EN 61000-4-2, EN 61000-4-3, EN 61000-4-4) Which follow the provisions o
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Warranty & Service Limited Warranty: Roper Scientific Analytical Instrumentation Roper Scientific, Inc. (“Roper Scientific,” us,” “we,” “our”) makes the following limited warranties. These limited warranties extend to the original purchaser (“You”, “you”) only and no other purchaser or transferee. We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary.
NTE/CCD Detector Manual Version 2.A VersArray (XP) Vacuum Chamber Limited Lifetime Warranty Roper Scientific warrants that the cooling performance of the system will meet our specifications over the lifetime of the VersArray (XP) detector or Roper Scientific will, at its sole option, repair or replace any vacuum chamber components necessary to restore the cooling performance back to the original specifications at no cost to the original purchaser.
Warranty & Service 49 Software Limited Warranty Roper Scientific warrants all of our manufactured software discs to be free from substantial defects in materials and / or workmanship under normal use for a period of one (1) year from shipment. Roper Scientific does not warrant that the function of the software will meet your requirements or that operation will be uninterrupted or error free.
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Index Accessories alignment of, 22 Aperture, 16 Background DC level, 33 Baseline signal, 33 Binning along columns, 13 along rows, 13 Bottom Clamps, 28 table of, 29 Calibration suitable light sources, 21 Cautions baseline signal shift, 34 IR contamination, 31 scintillator & UV, 33 CCD perpendicular mode operation, 12 square format, 13 Cleaning, 37 Cleaning optical surfaces, 37 C-mount, 28 adapters, 16 assembly, 28 lens installation/removal, 16 support recommendations, 28 Cold finger, 9 Contact information, 5
NTE/CCD Detector Manual NTE/CCD connecting the lens, 15 enclosures, 9 setup, 11 temperature range, 17 Optical path, 12 Outline drawings, 41 Overexposure, 16 Overexposure protection, 15 Parfocality, 29 Peltier effect, 17 Photodamage, 27 Power supply switches, 11 Quantum Efficiency (QE), 27 Readout pattern subtraction of, 33 Relay Lens, 28 Safety related symbols used in manual, 7 Setup cable connections, 11 power supply switches, 11 Shutter 25 mm, 36 drive selector, 35 entrance slit type of, 14 lifetime,
