User's Guide
Table Of Contents
- Digital LightCycler® System
- Safety
- User Assistance
- System description
- Overview of the system
- Overview of the instruments
- Overview of the software components
- Overview of the partitioning engine software
- Overview of the web application
- Overview of the analyzer software
- Overview of the development software
- Common software functions
- Overview of the supplies
- Overview of orders, run prerequisites, and results
- Maintenance overview
- Specifications
- Operation
- Overview of routine tasks
- Overview of diagnostic workflow
- Overview of development workflow without unassigned sample setup
- Overview of development workflow with unassigned sample setup
- Diagnostic workflow (quick reference)
- Development workflow without unassigned sample setup (quick reference)
- Development workflow with unassigned sample setup (quick reference)
- Frequently performed tasks
- Before routine operation
- Routine operation of diagnostic workflow
- Routine operation of development workflow
- Performing a development workflow without unassigned sample setup
- Performing a development workflow with unassigned sample setup
- Projects in the development software
- Run profiles in the analyzer software
- Entering run information for a development run in the analyzer software
- Runs in the development software
- About raw data files and runs
- Managing raw data files of development runs in the analyzer software
- Exporting raw data files of diagnostic runs from the web application
- Working with raw data files and runs in the development software
- Assigning partially matching unassigned sample setups to development runs in the development software
- List of columns on the Sample setup table view card
- Adding and editing lane settings of a run in the development software
- Working with sample groups in the development software
- Editing lane settings via copy and paste in the development software
- Clustering in the development software
- Analyses in the development software
- Unassigned sample setups in the development software
- About unassigned sample setups in the development software
- Working with unassigned sample setups in the development software
- Adding run settings and lane settings to unassigned sample setups in the development software
- Creating a sample setup report of an unassigned sample setup in the development software
- Analysis packages in the development software
- After routine operation
- Non-routine operation
- Overview of routine tasks
- Maintenance
- Maintenance
- Maintenance schedule
- Replacing the partitioning fluid bottle and/or the liquid waste bottle on the partitioning engine
- Data maintenance on the analyzer
- Cleaning and decontamination
- About preventive maintenance
- Maintenance
- Troubleshooting
- Flags
- Troubleshooting the partitioning engine
- Troubleshooting the analyzer
- Troubleshooting in the development software
- Troubleshooting in the web application
- Configuration
- User management in the analyzer software
- Administration of the analyzer software
- Settings in the development software
- Analytical principles
- Glossary
- Index
635
Roche Diagnostics
D
igitalLightCycler
®
System · Software version 1.0 · User Assistance · Publication version 1.1
– performing indel analyses, 398
–
plate view, 138
– projects, 294
– raw data files, 309
– run tabs, 130
– runs, 309
– Sample setup table view card, 323
– sample setup tabs, 136
– scatter plots, 132
– searching tables, 143
– sorting tables, 143
– specifications, 187
– starting, 240
– tab commands, 130
– tab navigation buttons, 129
– table view, 138
– tabs, 129
– unassigned sample setups, 402
– work area, 127
– working with analyses, 358
– working with analysis packages, 429
– working with raw data files, 317
– working with sample groups, 328
– working with unassigned sample setups, 405
Development software, columns
– Clustering method, 366, 395
– CN 68%CI max., 382
– CN 68%CI min., 382
– CN 95% CI max., 381
– CN 95% CI min., 381
– Conc. 68% max., 366
– Conc. 68% min., 366
– Conc. 95% CI max., 364
– Conc. 95% CI min., 364
– Concentration, 364
– Concentration mean, 370
– Concentration SD, 370
– Copies per nanowell, 366
– Copy number, 381
– Copy number mean, 385
– Copy number relative confidence, 383
– Copy number SD, 385
– Dilution factor, 323, 366, 395
– Dyes, 324
– Final result, 364, 381, 393
– Flags, 324, 341, 364, 381, 393
– for combinations of target of interest and master
mix reagent, 384
– for combinations of variant target and reference
target, 396
– for target combinations, 369
– for targets, 367
– Group, 370, 397
– Initial result, 365, 382, 394
– Lane, 340, 364, 367, 369, 381, 384, 393, 396
– Lane ID, 323
– Merged lanes, 365, 381, 394
– Merged lanes name, 325
– Merging, 325
– MMx, 324, 364, 370, 393, 397
– MMx (TOI), 380, 385
– MMx expiry date, 325
– MMx lot number, 325
– Negative nanowells, 366, 395
– No. of positive nanowells, 365, 394
– Notes, 324
– Plate type, 365, 394
– Pos. NWs, 340
– Quality indicator, 340
– Ratio, 382
– Ratio 68%CI max., 383
– Ratio 68%CI min., 383
– Ratio 95% CI max., 382
– Ratio 95% CI min., 382
– Raw concentration, 366
– Raw copy number, 383
– Raw ratio, 383
– Raw variant, 395
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