User's Guide
Table Of Contents
- Digital LightCycler® System
- Safety
- User Assistance
- System description
- Overview of the system
- Overview of the instruments
- Overview of the software components
- Overview of the partitioning engine software
- Overview of the web application
- Overview of the analyzer software
- Overview of the development software
- Common software functions
- Overview of the supplies
- Overview of orders, run prerequisites, and results
- Maintenance overview
- Specifications
- Operation
- Overview of routine tasks
- Overview of diagnostic workflow
- Overview of development workflow without unassigned sample setup
- Overview of development workflow with unassigned sample setup
- Diagnostic workflow (quick reference)
- Development workflow without unassigned sample setup (quick reference)
- Development workflow with unassigned sample setup (quick reference)
- Frequently performed tasks
- Before routine operation
- Routine operation of diagnostic workflow
- Routine operation of development workflow
- Performing a development workflow without unassigned sample setup
- Performing a development workflow with unassigned sample setup
- Projects in the development software
- Run profiles in the analyzer software
- Entering run information for a development run in the analyzer software
- Runs in the development software
- About raw data files and runs
- Managing raw data files of development runs in the analyzer software
- Exporting raw data files of diagnostic runs from the web application
- Working with raw data files and runs in the development software
- Assigning partially matching unassigned sample setups to development runs in the development software
- List of columns on the Sample setup table view card
- Adding and editing lane settings of a run in the development software
- Working with sample groups in the development software
- Editing lane settings via copy and paste in the development software
- Clustering in the development software
- Analyses in the development software
- Unassigned sample setups in the development software
- About unassigned sample setups in the development software
- Working with unassigned sample setups in the development software
- Adding run settings and lane settings to unassigned sample setups in the development software
- Creating a sample setup report of an unassigned sample setup in the development software
- Analysis packages in the development software
- After routine operation
- Non-routine operation
- Overview of routine tasks
- Maintenance
- Maintenance
- Maintenance schedule
- Replacing the partitioning fluid bottle and/or the liquid waste bottle on the partitioning engine
- Data maintenance on the analyzer
- Cleaning and decontamination
- About preventive maintenance
- Maintenance
- Troubleshooting
- Flags
- Troubleshooting the partitioning engine
- Troubleshooting the analyzer
- Troubleshooting in the development software
- Troubleshooting in the web application
- Configuration
- User management in the analyzer software
- Administration of the analyzer software
- Settings in the development software
- Analytical principles
- Glossary
- Index
490 List of flags
Roche Diagnostics
D
igitalLightCycler
®
System · Software version 1.0 · User Assistance · Publication version 1.1
Flag code Severity Description Actions
IP003
Informational No nanowell columns detected within the
expected overlap area during stitching
Restart the analyzer. If the flag occurs
repeatedly, contact your Roche Service
representative.
u Restarting the analyzer (451)
IP004
Critical Data integrity check failed
Restart the analyzer. If the flag occurs
repeatedly, contact your Roche Service
representative.
u Restarting the analyzer (451)
PE001
Critical Partitioning engine: Lane partitioning
failed
Restart the partitioning engine. If the flag
occurs repeatedly, contact your Roche Service
representative.
u Restarting the analyzer (451)
PE002
Informational Partitioning engine: Lane partitioning
data missing
Restart the partitioning engine. If the flag
occurs repeatedly, contact your Roche Service
representative.
u Restarting the analyzer (451)
PE003
Critical Partitioning engine: Empty lane
RA000
Critical Unhandled exception in results
interpretation.
Restart the analyzer. If the flag occurs
repeatedly, contact your Roche Service
representative.
u Restarting the analyzer (451)
RA001
Informational Upper limit of quantification not met.
RA002
Informational Lower limit of quantification not met.
RA003
Critical Positive control concentration is too high.
RA004
Critical Positive control concentration is too low.
RA005
Critical Negative control concentration is too
high.
RA006
Critical Internal control concentration is too high.
RA007
Critical Internal control concentration is too low.
RA008
Critical Too many invalid nanowells
If the flag occurs repeatedly, contact your
Roche Service representative.
RA009
Critical Integrity check failed.
If the flag occurs repeatedly, contact your
Roche Service representative.
RA011
Critical Too many artifact nanowells
Ensure there are no scratches, finger prints,
dust, or other artifacts on the nanowell plate
lanes that compromise the image integrity.
If the flag occurs repeatedly, contact your
Roche Service representative.
RA012
Critical Merging verification failed.
If the flag occurs repeatedly, contact your
Roche Service representative.
RA013
Critical Copy number control value is too high.
RA014
Critical Copy number control value is too low.
RA015
Critical TOI concentration is above upper limit of
validity.
RA016
Critical TOI concentration is below upper limit of
validity.
y Table of flags
18 Flags
Draft - Do Not Distribute