User's Guide

425Routine operation of development workflow
Roche Diagnostics
D
igitalLightCycler
®
System · Software version 1.0 · User Assistance · Publication version 1.1
Test details card
Card that lists the name of the master mix reagent, the
name of the variant target, and the name of the reference
target.
The Test details card is read-only.
Test settings card
Card to enter the minimum percentage of the variant
sequence, the limits of validity, and to configure the
display of final quantitative results.
Min. variant [%] field
Defines the minimum percentage of the variant
sequence that must be present in the sample for it to be
variant positive.
Precision = 4 decimal places
Limit of validity (for the total concentration of
the variant and the wild type) check box,
Lower limit of validity field, and Upper limit of
validity field
If the limit of validity is enabled, the sum of the measured
concentrations of the variant sequence and the wild-type
sequence must fall within the configured range.
If the concentration is outside the configured range, the
corresponding final (qualitative) result is invalid.
The lower limit of validity must be lower than the upper
limit of validity.
Precision = 4 decimal places
Show quantitative result check box
Enable or disable the calculation and display of the
percentage of the variant sequence (final quantitative
results).
If the check box is cleared, all final results are only
displayed qualitatively.
Default value = enabled
14 Routine operation of development workflow
Draft - Do Not Distribute