User's Guide

390 Analyses in the development software
Roche Diagnostics
D
igitalLightCycler
®
System · Software version 1.0 · User Assistance · Publication version 1.1
Analysis setup tab
On the Analysis setup tab, create the analysis setups:
• Choose the combinations of master mix reagent,
variant target, and reference target.
• Enter the minimum percentage of the variant
sequence.
• Optionally, enter the limits of validity.
MMx drop-down list
Lists the master mix reagents for which a sample was
included in the analysis on the Sample selection tab.
Variant drop-down list and Reference target
drop-down list
List the targets for which a sample was included in the
analysis on the Sample selection tab.
Variant target and reference target must be different:
If you choose a target from one of the drop-down lists, it
is not available anymore from the other drop-down list.
Min. variant [%] field
Defines the minimum percentage of the variant
sequence that must be present in the sample for it to be
variant positive.
Default value:
• Minimum percentage of the variant = 1.0000%
Limit of validity (for the total concentration of
the variant and the wild type) check box,
Lower limit of validity field, and Upper limit of
validity field
If the limit of validity is enabled, the sum of the measured
concentrations of the variant sequence and the wild-type
sequence must fall within the configured range.
If the concentration is outside the configured range, the
corresponding final (qualitative) result is invalid.
The lower limit of validity must be lower than the upper
limit of validity.
Default values:
• Limit of validity = enabled
• Lower limit of validity = 200.0000cp/µl
• Upper limit of validity = 500000.0000cp/µl
14 Routine operation of development workflow
Draft - Do Not Distribute