User's Guide

350 Clustering in the development software
Roche Diagnostics
D
igitalLightCycler
®
System · Software version 1.0 · User Assistance · Publication version 1.1
Adjusting the clustering settings and thresholds for manual clustering
For the manual clustering method, you can adjust the
pl
ot type, the enabled channels, and the axis ranges in
the clustering settings. In the scatter plots, you must
adjust the thresholds manually.
With the Manual clustering method, you can adjust the
t
hresholds manually in 1D scatter plots and 2D scatter
plots only.
A manual clustering via the Lasso button or the Spider
button cannot be used in the clustering settings of an
a
nalysis package.
r To adjust the clustering settings for
the Manual clustering method
1 I
n the development software, in the Navigation area,
choose the clustering from the Clustering menu.
f T
he clustering tab is displayed.
2 In the top right corner, choose the
button.
f The Settings combo box is displayed.
3 From the Clustering drop-down list, choose the
Manual option.
4 Fr
om the Graph drop-down list, choose an option:
•
1D scatter plot
• 2D scatter plot
I
The 3D scatter plot is for visualization purposes
o
nly. You cannot adjust the thresholds in a 3D
scatter plot.
5 In the Select channels table, enable the channels
t
hat you want to plot.
6 Optionally, in the Axis ranges fields, enter the
minimum and maximum values for the intensity axes
of the scatter plots. To reset the axis ranges, choose
the Reset button.
7 C
lick outside the Settings combo box. Alternatively,
c
hoose the
button.
f The values on the Clustering data card are
de
leted.
f The scatter plots are displayed.
8 Save the project.
9 Depending on the selected plot type, continue with
adjusting the thresholds in the scatter plots as
described in:
• To adjust the threshold in a 1D scatter plot
(
manual clustering) (351)
14 Routine operation of development workflow
Draft - Do Not Distribute