Specifications

OPERATION Photomicrography with
Carl Zeiss Color balance 35 mm SLR cameras
3-12 B 40-046 e 03/99
Assessing the Color Balance
• Make test exposures of a specimen area with as much empty background as possible in transmitted-
light brightfield.
• View slides on a standard light box, the light source of which has the correct illuminance and the
spectral energy distribution of 5500 K.
• The empty background in an exposure series should range from dark gray to middle gray, light gray
and white
Determine the Correction Filter
• Place filter of complementary color of the color distortion on the slide to be corrected.
Color distortion Color of the CC filter
Blue Yellow Y
Green Magenta M
Red Bluish green (Cyan) C
Yellow Blue B
Magenta Green G
Bluish green Red R
If, for example a filter of density 10 does not display the required color balance on viewing, you should
use a CC filter of half the density for the following exposure (i.e. 05 in this example). Filters of density
CC - 05 to CC 10 are usually sufficient for correction.
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Perfectly corrected color exposures require the microscope setting, the development process
and the film batches to remain unchanged.