User manual

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XXPRESS
USER MANUAL - 1.1
In the third step the temperature is increased to 72°C, the ramp
rate is again 10°C per second. Once the temperature is at 60°C
then this value is held for 5 seconds. At the end of this dwell there
is a fluorescence measurement made using the channel 1 filter
settings.
The temperatures for each step can be specified anywhere
between 40°C and 99°C in 0.1°C steps.
The thermal ramp rate can be specified for each step
independently or set as a
GLOBAL RAMP
rate using the tile on the
right of the pop-up screen. Value can be set from zero to 10°C per
second in 0.1°C steps.
The number of times the cycle should be run in is set in the
REPEAT
tile, in the example in Figure 5a the 3 step cycle is
repeated forty times.
Touch the
tick to accept these conditions or the cross to
reject.
For experiments where multiple cycles are required these can be
added by touching the “plus” symbol and editing them in the
same manner. Unwanted steps can be deleted using the
“minus” symbol
The final segment sees the temperature being reduced to 45°C
and held for 10 seconds to complete the experiment.
Based on the chemistry choice xxpress will select a
recommended sensitivity for the camera that measures the
fluorescence, this is a combination of gain and exposure time.
Touching the blue
CAMERA
tile will pop-up a window that will allow
the sensitivity of the measurements in each of the five
fluorescence channels to be individually adjusted, see Figure 5b.
To adjust touch the circle and then drag it to the position along the
line that gives the desired value. The value range is from zero to
100. (To drag the circle maintain finger contact with the touch
screen.)