User manual

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XXPRESS
USER MANUAL - 1.1
• Looking at our example 3-Step PCR, we chose a SYBR Green
based test and Kapa SYBR 2G Fast Hot Start chemistry. In Figure
5 the thermal profile reflects this with an initial hot start at 95°C for
20 seconds followed by a 3-Step cycle which is repeated 40 times.
Finally there is a cooling step to bring the plate down close to
ambient enabling safe removal from the unit.
• Touching each part of the profile will pop-up a screen allowing the
parameters to be modified, see Figure 5a.
Figure 5a
• Select the parameter to be changed, here we have selected the
dwell time on the second step of the cycle which is now
highlighted. Then type in the desired value or select the option
required using the keypad on the right of the display.
Once the user is happy with their changes they can save them by
touching the tick symbol or cancel and lose their changes
by touching the 

 cross.
• In the example in Figure 5a there are 3 steps to the PCR. Initially
the temperature is raised to 95°C at a ramp rate of 10°C per
second. Then the temperature is held at that value for 1 second.
• In the second step the temperature is reduced to 60°C, the ramp
rate is again 10°C per second. Once the temperature is at 60°C
then this value is held for 5 seconds.