User guide

Quantity One User Guide
4-4
3. Select the output.
4.2 Step I. Position Gel
The Chemi Doc window will open in “live” mode, giving you a live video
display of your sample. In this mode, the Live/Focus button will appear
selected, and frames will be captured and displayed at about 10 frames per
second, depending on the speed of your computer.
You can use live mode to zoom, focus, and adjust the aperture on the camera,
while positioning the sample within the area.
Note: Newer versions of the Chemi Doc feature a motorized zoom lens that can
be controlled directly from the acquisition window using the Iris, Zoom,
and Focus arrow buttons. Click on the Up/Down buttons while viewing
your sample in the window to adjust the lens. These buttons will not be
visible if you are connected to older versions of the Chemi Doc without
the motorized zoom lens.
Fig. 4-3. Live/Focus button and camera control buttons.
You can also select the Show Alignment Grid checkbox to facilitate
positioning.
Note: After positioning your sample, you should check the Imaging Area
dimensions under Options (see below) to make sure that they conform to
the size of the area you are focusing on. To determine the size of the area
you are focusing on, you can place a ruler in the Chemi Doc box so that it
is visible by the camera.