User guide

Contents
vii
14. Bands ................................................................................ 14-1
14.1. How Bands Are Identified and Quantified .......................................... 14-2
14.2. Automatically Identifying All Bands .................................................... 14-3
14.3. Identifying and Editing Individual Bands ......................................... 14-10
14.4. Plotting Traces of Bands ....................................................................... 14-14
14.5. Band Attributes ...................................................................................... 14-15
14.6. Displaying Band Information .............................................................. 14-18
14.7. Gauss-Modeling Bands ......................................................................... 14-21
14.8. Irregularly Shaped Bands in Lanes ..................................................... 14-25
15. Standards and Band Matching ....................................... 15-1
15.1. Defining and Applying Standards ........................................................ 15-2
15.2. Band Matching ....................................................................................... 15-13
15.3. Quantity Standards ................................................................................ 15-23
16. Volume Tools ................................................................... 16-1
16.1. Creating a Volume Object ....................................................................... 16-1
16.2. Moving, Copying, and Deleting Volumes ........................................... 16-6
16.3. Volume Standards ................................................................................... 16-7
16.4. Volume Background Subtraction .......................................................... 16-9
16.5. Volume Arrays ....................................................................................... 16-11
17. Colony Counting .............................................................. 17-1
17.1. Defining the Counting Region ............................................................... 17-2
17.2. Counting the Colonies ............................................................................. 17-4
17.3. Displaying the Results ............................................................................ 17-5
17.4. Making and Erasing Individual Colonies ............................................ 17-6
17.5. Using the Histogram to Distinguish Colonies ..................................... 17-6
17.6. Ignoring a Region of the Dish ................................................................ 17-8
17.7. Saving/Resetting Your Count ............................................................. 17-10
17.8. Saving to a Spreadsheet ........................................................................ 17-10