User guide

Gel Doc
3-5
the size of the area you are focusing on. To determine the size of the area
you are focusing on, you can place a ruler in the Gel Doc box so that it is
visible by the camera.
3.3 Step II. Acquire Image
For many white light applications, you can skip this step and save and print
images directly from Live/Focus mode.
For UV light, chemiluminescent applications, or faint samples, you can take
an automatic exposure based on the number of saturated pixels in the image
or you can enter a specific exposure time.
Note: “Exposure” refers to the integration of the image on the camera CCD over
a period of time. The effect is analogous to exposing photographic film to
light over a period of time.
Auto Expose
Auto Expose will take an exposure whose time length is determined by the
number of saturated pixels in the image. This is useful if you are uncertain of
the optimal exposure length.
Note: If you know the approximate exposure time you want (± 3 seconds), you
can skip this step and go directly to Manual Expose.
Click on the Auto Expose button to cancel Live/Focus mode and begin an
automatic exposure. The Auto Expose button will appear selected throughout
the exposure.
During the auto exposure, the image is continuously integrated on the camera
CCD until it reaches a certain percentage of saturated pixels. This percentage
is set in the Options dialog box. (Default = 0.75 percent. See Options below.)