User guide

General Operation
2-21
functions, while experienced users can specify a longer delay once they are
familiar with the icons.
Tool Help Persistence determines how long the Tool Help will linger on the
screen after you move the cursor off a toolbar icon.
2.5.c Application
Click on the Application tab to access the following preferences.
Relative Quantity Calculation
The Relative Quantity Calculation option allows you to define how the
quantities of defined bands in lanes will be determined for all reports,
histograms, and band information functions: either as a percentage of the
signal intensity of an entire lane or as a percentage of the signal intensity of
the defined bands in a lane.
Selecting % of Lane means that the total intensity in the lane (including bands
and the intensity between bands) will equal 100 percent and the band that
you select will be reported as a fraction thereof.
Selecting % of Bands in Lane means that the sum of the intensity of the
defined bands in a lane will equal 100 percent, and your band will be reported
as a fraction of that sum.
If you create, adjust, or remove bands with Relative Quantity defined as % of
Bands in Lane, the relative quantities of all the other bands in the lane will
update to reflect changes in the total intensity of defined bands.
Relative Front Calculation
The Relative Front Calculation option lets you select the method for
calculating the relative positions of bands in lanes . This affects the calculation
of both Relative Front and Normalized Rf values.
Relative front is calculated by either: