User guide

Quantity One User Guide
18-2
Normalization
To assign a normalized band type, select Normalize from the Match menu
and then click on a matched band that appears in every lane of your gel. The
quantity of that band in each lane will be set to 100, and the quantities of the
other bands in the lane will be normalized to that band. (If the band is not
present in a lane, the normalized quantity for that lane is zero.)
See section 15.2.d for more information.
18.1.a Differential Display Searches
After you have a assigned a normalized band type, select Differential Display
from the Analysis menu. A box will open in which you can specify your
search parameters.
Fig. 18-2. The Differential Display dialog box.
You can perform two types of searches on bands across a differential display
gel: an Outlier search or a Trend search. Both are determined by the
Percentage and Quantity values that you specify in the Differs By field.
Select the Outlier button to search across each band type for bands with a
normalized quantity that differs from the mean normalized quantity for that
band type. An outlier is defined as a band with normalized quantity (n
q
) that
satisfies one of the following criteria: