User guide
Colony Counting
17-9
Click on the Ignore Region button, then position your cursor on one edge of
the region you want to ignore. Drag your cursor on the image, defining the
full region you want to ignore.
Fig. 17-7. Marking a region to ignore.
As you drag, you will create a “pie slice” marked with red cross-hatching.
Any colonies in this region will not be considered in the final count.
When you have defined the region, release the mouse button. If you want to
change the size of the ignored region, position your cursor on the edge of the
pie slice near the rim of the blue circle. Your cursor will change to a
bidirectional arrow, and you can drag the edge of the pie slice.
Colony Count and Adjusted Count
After you have defined a region to ignore, two different counts will appear in
the dialog box: the colony count and the adjusted count.
The colony count is the number of colonies that appear in the defined circle
minus those in the ignored region.