User guide

Quantity One User Guide
17-6
17.4 Making and Erasing Individual Colonies
If automatic colony detection has missed or erroneously detected some
colonies, you can manually mark or unmark them directly on the image using
the buttons under Tools/Options.
Fig. 17-4. Colony counting tools.
To mark a colony, click on the Make Colony button, then click on the spot on
the image that you want to identify as a colony.
To unmark a colony, click on the Erase Colony button, then click on the colony
on the image that your want to unmark.
Your colony count will change accordingly.
17.5 Using the Histogram to Distinguish
Colonies
The histogram in the Colony Counting dialog box is a graphical
representation of the signal data in the image. You can use the histogram and
associated sliders to reduce the number of incorrectly identified colonies
and/or distinguish between white and blue colonies in the image.