User guide

Quantity One User Guide
16-2
Fig. 16-1. Volume tools.
Note: When using any of the following tools, be careful to completely surround
the data you want to quantitate. You should also adjust for background
intensity (see section 16.4 below). You may want to experiment with
several different volumes drawn around the same object before selecting
the one that gives you the best quantitation data.
Volume Contour Tool
The Volume Contour tool will quickly create a volume boundary that follows
the outer edge of the object you want to quantify. To use this tool, first
magnify the object using the Zoom tools, then click on the Volume Contour
button.
• If you click on a pixel at the edge of the object, you will create a contour
that encloses pixels whose intensity is equal to or greater than that of the
pixel at the cursor.
• If you click and drag the mouse when creating a contour, the shape of the
contour will change as you move over pixels of different intensity. Drag
from inside the object outward until the contour follows the outer edge of
the object. When you release the mouse button, the volume is created.