User guide

16-1
16. Volume Tools
You can use the Volume tools to quantitate bands, spots, arrays, and other
image data.
What is a Volume?
A volume is the intensity data inside a defined boundary drawn on your
image.
Volume = Sum of the intensities of the pixels within the volume
boundary x pixel area
Volume units = intensity units x mm
2
Volumes are similar to band contours (see section 14.8.a), except that they are
not dependent on lanes and bands.
To measure the amount of a particular object in an image, you draw a volume
rectangle, contour, freehand, or circle around the object and compare the
intensity data inside the boundary with the data of other objects or a standard
using the Volume Analysis Report and Volume Regression Curve.
Note: The Volume Analysis Report and Volume Regression Curve are described
in detail in sections 19.5 and 19.6, respectively.
16.1 Creating a Volume Object
To create a volume, open the Volume Tools by clicking on the Volume Overlay
Tools button on the main toolbar, or selecting Volume Overlay Tools from the
Edit menu. (These commands are also located on the Volume menu.)