User guide

Standards and Band Matching
15-31
In the Quantity Standards dialog box, next to the band that came from the
undiluted stock solution, enter the known quantity.
In the Dilution Factor column, type “stock.”
Next to each of the other bands in the dialog box, enter the dilution factor in
the Dilution Factor column.
Fig. 15-23. Entering a dilution series.
The quantity of each band will be automatically calculated.
15.3.e Importing a Calibration Curve
A calibration curve created on one gel image can be applied to bands on other
images.
Make sure that both the image you want to quantitate and the image with the
calibration curve are currently open.
Click on the image you want to quantitate. Select Match > Quantity Standards
and click on “create new.”
In the Quantity Standards dialog box, click on the Import Curve button. From
the list displayed, choose the calibration curve you want to use to quantitate
bands on the image.