User guide

Quantity One User Guide
15-30
15.3.c Applying the Calibration Curve
Once you have selected known bands and entered values for them, you are
ready to choose the bands whose quantities you want to calculate with the
calibration curve. This is done using the buttons found next to the Calibrate
prompt.
Fig. 15-22. Buttons for calibrating bands of unknown quantity.
• To quantitate individual bands, click on Band, then click on the band of
interest.
• To quantitate all the bands in a matched group, click on Match, then click
on any of the bands in the matched group.
• To quantitate all the bands in a lane, click on Lane, then click on a lane of
interest.
• To quantitate all the bands in the image, click on Gel.
Calibrated bands are highlighted after they are selected.
Unapplying the Calibration Curve
To undo the calibration of a band, match, lane, or gel, use the corresponding
command next to the Un-Calibrate prompt.
15.3.d Generating Standard Bands via a Dilution Series
One way to get a set of bands of known quantity is by starting with a stock
solution and making several dilutions from it. A different dilution can be
loaded onto each lane of a gel, resulting in a group of bands whose quantities
can be calculated from the known quantity used to make up the stock
solution and the dilution factor.
Quantity One will perform these calculations for you if you provide it with
the known quantity and the dilution factor (e.g., for a solution that has been
diluted to 10 times the volume, type 1/10 or 0.1).