User guide

Quantity One User Guide
15-26
Selecting Bands of Known Quantity
The dialog box includes three buttons that are used to select bands on the gel
image with which to generate the calibration curve. These buttons are located
next to the Select prompt.
Fig. 15-19. Buttons for selecting bands of known quantity.
• To select bands one at a time, click on the Band button, then click on each
band of known quantity. Each band will be highlighted.
• If all of the bands are the same band type, click on the Match button, then
click on one of the bands in the matched group. The group will be
highlighted.
• If all of the bands are in one lane, click on the Lane button, then click on
the lane with the known quantities. The entire lane will be highlighted.
In the lower part of the Quantity Standards dialog box, the lane and band
numbers of the selected bands will appear in the Band column. The values
derived from their signal intensities are also displayed.
Entering the Quantity Standards
After selecting the bands to be used as quantity standards, enter their
quantities in the appropriate fields in the Quantity column.
After you have entered each quantity, the band status in the Status column
changes from Unknown to Known.