User guide

Standards and Band Matching
15-21
Propagate Band Set is a feature that not only simplifies assigning band types,
it allows the software to do some optimizations that will significantly speed
up modeling.
Choose a reference sample lane that you want to apply the band set to. Start
by assigning one or two band types in the lane that are easy to identify, then
click on the Propagate Band Set button and click on the lane to assign the
remaining band types to the lane.
Propagate Band Set assigns the band types one at a time. The command will
assign a type to a band only if it is very sure that it is the correct assignment.
Once your reference sample lanes have been modeled, add any unknown
band types to the gel using the methods outlined in section 15.2.a above.
15.2.d Normalizing for Quantity
You can normalize the quantities of the bands in your image to the quantity of
a particular matched band that appears in all lanes. This is useful if you have
loaded different amounts of sample in each lane.
Note: Quantity normalization is required for calculating Differential Display.
Select Normalize from the Match menu and click on a matched band that
appears in every lane. (If the band is not present in a lane, the normalized
quantity for that lane will be zero.) The quantity of that band will be set to 100
in each lane, and the quantities of the other bands will normalized to that
band.
You can view the normalized quantities using the Band > Band Attributes
command.
15.2.e Graphs of Match Data
You can display graphs of different kinds of data associated with your
matched bands. The commands for displaying these are located on the Match
> Match Graphs submenu.